Dr. Nadeem Ahmad Siddique has contributed significantly in both exploratory and authoritarian of natural drug discovery, pharmacological evaluation and development of mechanism base drug delivery research. Significantly contributed in, in-vitro and in-vivo pharmacological screening of natural products and their formulations for nephroprotection, hepatoprotection, preparative isolation, characterization of bioactive marker compounds from natural sources, expansion of extraction technology and natural product repository. He has mainly contributed in nephroprotection, hepatoprotection, aflatoxins characterization by newer hyphenated techniques with therapeutic indications in the following areas: Bioanalysis and method development: Dr. Nadeem Ahmad Siddique developed and validated a new bioanalytical method for simultaneous determination of aflatoxins by High-Performance Liquid Chromatography–Tandem Mass Spectrometry (recommended by US-FDA/WHO) which is of immense utility for found to be suitable for the rapid and accurate assessment of aflatoxin levels in various plant and their formulations. The developed method could be applied to determine the aflatoxin contamination in a cost and time effective manner. The output obtained from this monitoring can be used as a basis for risk analysis of aflatoxin contamination in health care (Siddique et al., J Pharm Pharm Sci, 2013). Many faster, cheaper, more sensitive and reliable quantitative bioanalytical methods were developed in the smallest possible volume of biological samples with as few sample preparation steps as possible and showing negligible biomatrix effect and validated for employing them in different experimental therapeutic studies (Siddique et al.,J Natural Product Research, 2011). Dr. Nadeem is employing hyphenated techniques for bioanalytical quantitative determination of various synthetic and natural origin, marketed drugs and their metabolites, herbal components/fractions from extracts/multicomponent herbal preparations and herbal nutraceuticals, for studying their fate in biological systems using in vivo, in vitro, ex vivo and in situ assays mainly in nephrpoprotection, and hepatoprotection therapeutic indications (Siddique et al.,WJPPS, 2014). Dr. Nadeem has also been involved in the analytical research by developing the reliable analytical methods for the detection of heavy metal toxins such, arsenic, mercury, lead, cadmium in plant extracts to assess cumulative exposure even at nanomolar concentrations (Siddique et al.,IntJPRes, 2013). To insure, safety and quality value of drugs Dr. Nadeem proposed a cost efficient, flexible and rapid method for estimation of heavy metals, pesticide residues and aflatoxins as they are frequently consumed throughout the community without of their safety awareness. Experimental Therapeutics: Dr. Nadeem also reported that high performance thin layer chromatography (HPTLC) is another choice for analytical chemist and widely used for dealing with herbal preparations. HPTLC with densitometry has been employed for detection of adulteration in THMs (Siddique et al.,DTA, 2013). It offers effective separation of analytes and their detection through relative retention time (RRT) matching with the standard pro- vided. Hyphenated-mass spectrometric techniques have been utilized for separation and detection of target aflatoxin for which THMs claims on labels (Siddique et al., IntJPRes, 2013). He explored the role of various aminoglycosides and anticancerous agent and their mechanistic nephrotoxicity. Hitherto unknown, Dr. Nadeem has proposed that the nephroprotective activities due to presence of phytochemicals like phenolics and flavonoids (Siddique et al.,AJPS, 2010). This finding would help in making informed decision to prevent adulteration and role of bioactive constituents and therapeutic value in nephroprotection and hepatoprotection (Siddique et al.,IJCP, 2011). In-vitro and in-vivo nephroprotective potential were evaluated through an adapted GGT assay method. Inhibitory studies were carried on GGT to find out the effect of mesna–disulfde heteroconjugates on the enzyme’s action. The mesna by pass GGT metabolism pathway and not be the substrate for GGT xenobiotic pathway. In distinguish to this mesna comprise inhibitory effect on GGT. Developed method offered significant positive effect on blood urea, uric acid and serum creatinine levels as well TBARS, SOD, GSH and catalase activity at a dose of 800mg/kg b.w. as it shows more phenolics, high percentage inhibition and low IC50 (Siddique et al., WJPPS, 2014).

PHARMACEUTICAL SCIENCES(PHARMACOGNOSY