Research Article
Volume 5 Issue 1 - 2020
Effect of SN-6 on the Motility in the Circular Smooth Muscles of Mouse Ileum
Yasu-Taka Azuma*, Natsuho Yoshida and Kazuhiro Nishiyama
Laboratory of Veterinary Pharmacology, Division of Veterinary Science, Osaka Prefecture University Graduate School of Life and Environmental Sciences, Izumisano, Osaka, Japan
*Corresponding Author: Yasu-Taka Azuma, Laboratory of Veterinary Pharmacology, Division of Veterinary Science, Osaka Prefecture University Graduate School of Life and Environmental Sciences, Izumisano, Osaka, Japan.
Received: December 18, 2019; Published: December 27, 2019




Abstract

The Na+/Ca2+ exchanger (NCX) is a plasma membrane transporter involved in regulating intracellular Ca2+ concentrations. NCX is critical for Ca2+ regulation in cardiac muscle, vascular smooth muscle, and neurons. We showed that NCX1 and NCX2 play an important role in the gastrointestinal motility using NCX1 heterozygous, NCX2 heterozygous, and NCX1.3 transgenic mice. Researches using genetically modified animals are important, but it is more important to demonstrate the effects of inhibitors for drug therapeutics. To determine the role of SN-6, an NCX inhibitor, in gastrointestinal tissues, we examined electric field stimulation (EFS)-induced responses in the circular smooth muscles of the ileum. We found that EFS-induced contraction that persisted during EFS were smaller in the presence of SN-6 than in control. In the experiments in which L-NNA was added following the EFS, EFS-induced contraction was still smaller in the presence of SN-6 than those in control. Under the treatment of atropine, EFS-induced relaxation in the presence of SN-6 was similar to that of control. On the response of smooth muscles, SN-6 did not affect CCh-induced contraction nor NOR-1, which generates NO, induced relaxation. In this study, we demonstrate that it may be possible to use NCX inhibitor as a gastroprokinetic agent.

Keywords: Acetylcholine; Contraction; Ileum; Myenteric Neurons; Solute Carrier Family 8 Member A

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Citation: Yasu-Taka Azuma., et al. “Effect of SN-6 on the Motility in the Circular Smooth Muscles of Mouse Ileum”. EC Nutrition 5.1 (2020): 01-09.

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