Research Article
Volume 7 Issue 6 - 2022
Prevalence and Characterization of Staphylococci from Dried Crayfish in Selected Markets in FCT, Abuja, Nigeria
E Godwin1*, Okolocha EC2, I Annette3, Enem SI1 and Okoli CE1
1Department of Veterinary Public Health and Preventive Medicine, University of Abuja, Nigeria
2Department of Veterinary Public Health and Preventive Medicine Ahmadu Bello University Zaria
3National Agricultural extension and research liaison services Ahmadu Bello University Zaria
*Corresponding Author: E Godwin, Department of Veterinary Public Health and Preventive Medicine, University of Abuja, Nigeria.
Received: April 04, 2022; Published: May 25, 2022




Abstract

Staphylococci spp with special reference to Staphylococcus aureus play an important role in foodborne diseases and it is one of the most important pathogens found in sea foods. Staphylococcus aureus is considered as one of the most frequently occurring food-borne pathogen worldwide and cause food poisoning. The risk of zoonotic transmission to humans highlights the need to understand the prevalence and antibiogram of staphylococcus aureus in dried crayfish sold at selected markets in FCT, Abuja Nigeria. A cross sectional epidemiological study method was adopted in the study while a multistage technique was used to select the study sites and sample subjects. A total of 400 dried crayfish samples were collected from six different markets (Wuse, Garki, Gwagwalada, Zuba, Kubwa and Bwari). Samples were collected from June to September 2017 and analyzed using standard procedures on Baird Parker (BP) agar plates with supplements of egg yolk tellurite emulsion. Staphylococcus species were identified using conventional biochemical tests. Isolates were further characterized using Microgen TM STAPH-12S KIT. The antimicrobial sensitivity test of the isolates were carried out using disc diffusion method on Mueller Hinton agar and was interpreted according to clinical and laboratory standard institutes. Polymerase Chain Reaction was carried for the detection of MecA gene. The prevalence of staphylococcus species isolated from samples indicated (9) 34.6% for staphylococcus chromogenes, (8) 30.8% for staphylococcus aureus, (4) 15.4% for staphylococcus hyicus, (4) 15.4% for staphylococcus xylosus and staphylococcus sciuri (1) 3.8%. The antibiogram of staphylococcus species from the isolates revealed high sensitivity to some of the antibiotics used such as vancomycin (100%) imipenem (100%) and gentamycin 92.3% while resistance was exhibited for oxacillin (96.2%) and penicillin (76.9%) and 53.8% for cefoxitin. The presence of MecA gene was detected among the staphylococcus aureus isolates. The findings from this research revealed that dried crayfish samples from different markets harboured staphylococcus aureus amongst other staphylococci which is potentially pathogenic and constitutes an important source of food-borne infection and intoxication. Proper handling, processing, packaging, storage, distribution and transportation methods should be put in place to reduce potential health risks.

 

Keywords: Prevalence; Antibiogram; staphylococcus species; Crayfish

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Citation: E Godwin., et al. “Prevalence and Characterization of Staphylococci from Dried Crayfish in Selected Markets in FCT, Abuja, Nigeria”. EC Veterinary Science 7.6 (2022): 30-41.

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