Research Article
Volume 7 Issue 6 - 2022
Toxicological Effects of Neem Seed Oil and Neem Seed Kernel Water Extract (Azadirachta Indicia) on Broiler Chickens in Khartoum State, Sudan
Mona Abdelrahman Mohamed Khaier1* Sara Elgaili Ali Ibrahim2, Amal Mustafa3 and Elamin Elrayah4
1Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan
2Department of Pathology, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan
3Poultry Department, Central Veterinary Research Laboratories, Khartoum, Sudan
4Zoology Department, Faculty of Science, University of Khartoum, Khartoum, Sudan
*Corresponding Author: Mona Abdelrahman Mohamed Khaier, Department of Molecular Biology and Bioinformatics, College of Veterinary Medicine, University of Bahri, Khartoum, Sudan.
Received: March 16, 2022; Published: May 25, 2022


Aim and Background: Neem tree is a medicinal plant having anti-coccidian effect in broilers and it is used as a pesticide and acaricide. Based on these facts this study aims to detect the toxicological effect of the Neem seed oil and Neem water extract on broiler chickens.

Material and Methods: Forty Bovan chicks were housed within the premises of the Veterinary Laboratories at Soba. At the age of 30 days they were grouped into 8 groups. The groups were used for feeding (Neem seed powder) and dipping (Neem seed oil and Neem water extract) tests with two concentrations of Neem seed powder, Neem seed oil and Neem water extract (0.0, 0.5% and 15%) for one-week period. The average weight of the chicks before and after feeding and dipping was recorded. Then chicks were slaughtered a week after feeding and dipping. Specimens from the liver, kidney and brain were fixed in 10% formalin for Histopathological examination.

Results: The gain in weight for the control group was 0.241 grams for a week’s time while the gain in weight for chicks fed on 0.5% Neem seed powder was 0.417 grams and 0.945 grams for that fed on 15% Neem seed powder. The groups fed or dipped in Neem seed powder, Neem oil and Neem seed kernel water extract perform no clinical signs or post-mortem changes in chick’s internal organs (liver, kidney and brain); however, some Histopathological changes seen may be reversible. The severity of these Histopathological changes were less when compared with those induced by chemical pesticides. Conclusion: The Neem seed kernel powder fed to chicks increase the body weight as the concentration increase. Neem oil and Neem seed kernel water extracts induce no clinical signs or post-mortem changes in chick’s internal organs (liver, kidney and brain) when fed or dipped with different concentrations.

Keywords: Bovan Chicks; Dipping; Feeding; Histopathology; Neem Oil and Neem Water Extract


  1. Al‐Rajhy DH., et al. “Acaricidal effects of cardiac glycosides, azadirachtin and neem oil against the camel tick, Hyalomma dromedarii (Acari: Ixodidae)”. Pest Management Science: formerly Pesticide Science11 (2003): 1250-1254.
  2. Esonu O., et al. “Physiological response of laying birds to Neem (Azadirachta indica) leaf meal-based diets: body weight organ characteristics and haematology”. Online Journal of Health and Allied Sciences2 (2006).
  3. Seddiek SA., et al. “The acaricidal efficacy of aqueous neem extract and ivermectin against Sarcoptes scabiei var. cuniculi in experimentally infested rabbits”. Parasitology Research6 (2013): 2319-2330.
  4. Khaier AM., et al. “Effects of Neem (Azadirachta indica) Seed Kernel Powder on Tribolium castaneum, the Intermediate Host of Some Poultry Cestodes”. Human 14.3 (2019): 93-100.
  5. Dogo GA., et al. “Therapeutic Evaluation of Neemazal® Against Experimental Eimeria Tenella Infection in Broiler Chickens, Jos-Nigeria”. Journal of Parasite Research2 (2020): 15-25.
  6. Vinus RD., et al. “Potential benefits of herbal supplements in poultry feed: A review”. The Pharma Innovation Journal6 (2018): 651-656.
  7. Ezzat HN., et al. “A review on the effects of neem (Azadirachta indica) as feed additive in poultry production”. Journal of Entomology and Zoology Studies1 (2018): 1331-1333.
  8. Luna LG. Manual of histologic staining methods of the Armed Forces Institute of Pathology.
  9. Olabode AD. Performance, egg quality characteristics and serum biochemistry of laying birds fed diet containing Neem leaf Meal (Doctoral dissertation) (2017).
  10. Murphy DJ. “The biogenesis and functions of lipid bodies in animals, plants and microorganisms”. Progress in Lipid Research5 (2001): 325-438.
  11. Gowda SK and Sastry VRB. “Neem (Azadirachta indica) seed cake in animal Feeding-Scope and Limitations-Review”. Asian-Australasian Journal of Animal Sciences5 (2000): 720-728.
  12. Jacobson M and Schmutterer H. “Toxicity of Neem to Vertebrates and Side Effects on Beneficial and Other Ecologically Important Non‐Target Organisms”. The Neem Tree: Azadirachta indica A. Juss. and Other Meliaceous Plants (1995): 484-517.
  13. Seddiek SA., et al. “The antitrichomonal efficacy of garlic and metronidazole against Trichomonas gallinae infecting domestic pigeons”. Parasitology Research4 (2014): 1319-1329.
  14. Seddiek, SA., et al. “Anthelmintic activity of the white wormwood, Artemisia herba-alba against Heterakis gallinarum infecting turkey poults”. Journal of Medicinal Plants Research16 (2011): 3946-3957.
  15. Khater HF. “Introductory chapter: Back to the future-solutions for parasitic problems as old as the pyramids”. Natural Remedies in the Fight Against Parasites (2017): 3-19.
  16. Khater HF., et al. “Commercial mosquito repellents and their safety concerns, In: Malaria”. Intech Open (2019).
  17. Baz M., et al. “Plant oils in the fight against the West Nile Vector, Culex pipiens”. The International Journal of Tropical Insect Science (2022).
  18. Khater HF and Geden CJ. “Efficacy and repellency of some essential oils and their blends against larval and adult house flies, Musca domestica L.(Diptera: Muscidae)”. Journal of Vector Ecology2 (2019): 256-263.
  19. Seddiek SA., et al. “The acaricidal efficacy of aqueous neem extract and ivermectin against Sarcoptes scabiei var. cuniculi in experimentally infested rabbits”. Parasitology Research6 (2013): 2319-2330.
  20. Ali A., et al. “Effect of butyrate, clopidol and their combination on the performance of broilers infected with Eimeria maxima”. British Poultry Science4 (2014): 474-482.
  21. Ali M., et al. “Comparative efficacy of synbiotic and diclazuril on broilers experimentally infected with Eimeria acervuline”. Assiut Veterinary Medical Journal146 (2015): 24-33.
  22. Osman OA. Seed Toxicity to Rats and Chicks (Doctoral dissertation, University of Khartoum) (2001).
  23. Elham M Osman. “Studies on some Acaricides, M.V.Sc”. Thesis, University of Khartoum, Sudan (1993).
Citation: Mona Abdelrahman Mohamed Khaier., et al. “Toxicological Effects of Neem Seed Oil and Neem Seed Kernel Water Extract (Azadirachta Indicia) on Broiler Chickens in Khartoum State, Sudan”. EC Veterinary Science 7.6 (2022): 11-19.

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