Research Article
Volume 7 Issue 5 - 2022
Effects of NSPase Supplementation on Growth Performances, Histological and Macroscopic Lesions and Coccidiosis Load of Broiler Chickens

Sophia DERQAOUI1*, Yassmina BIDOUDAN1, Ibrahim EL IDRISSI3, Abderrahmane JANNOUNE4, Mariam NACIRI5, Mohammed OUKESSOU2 and Saadia NASSIK1

1Unit of Avian Pathology, Department of Veterinary Pathology and Public Health, Agronomy and Veterinary Medicine Institute Hassan II, Rabat-Instituts, Rabat, Morocco

2Unit of Physiology and Therapeutics, Department of Veterinary Biological and Pharmaceutical Sciences, Agronomy and Veterinary Medicine Institute Hassan II, Rabat-Instituts, Rabat, Morocco

3Vétérinaire Conseil Azemmour, Morocco

4Animal Genetic Expert, App.B, Imm, Avenue Hassan II, Agdal, Rabat, Morocco

5Research Center (BIOBIO), Laboratory of Biodiversity, Ecology and Genome, Faculty of Sciences, Mohamed V University, Rabat, Morocco

*Corresponding Author: Sophia DERQAOUI, Unit of Avian Pathology, Department of Veterinary Pathology and Public Health, Agronomy and Veterinary Medicine Institute Hassan II, Rabat-Instituts, Rabat, Morocco.
Received: March 21, 2022; Published: April 25, 2022




Abstract

The development and use of enzymes has gained momentum in poultry nutrition as an alternative to growth-promoter antibiotics use. Non-starch polysaccharide degrading enzymes (NSPase) prevent the accentuation of viscosity in the gastrointestinal tract and its associated negative effects, which will allow better digestion and absorption of nutrients, reduced microbial proliferation and healthier gut. The objective of this study is to evaluate the effects of several NSPases on growth performances, intestinal histomorphology, macroscopic lesions and coccidiosis loads of broiler chickens. A total of 2400 one-day-old male chicks (Ross 308 strain) were weighted and randomly assigned to 80 floor pens at the rate of 30 birds/pen. A total of 10 treatments have been tested with 8 replicates each. Eight groups received basal diets supplemented with β-xylanase, β-glucanase, α-amylase, xylanase, cellulase, mannanase and α-galactosidase at different rates. Two control diets were formulated to meet the nutrient requirements: 1) positive control (PC) (diet with Avilamycine) and negative control (NC) (diet, without antibiotic and NSPases). The main results showed that supplementation of 50 g of β-xylanase and β-glucanase mixture (T3) induced significant (p < 0.05) body weight (BW) and body weight gain (BWG) increases and a very highly (p < 0.001) significant decrease in feed conversion ratio (FCR) during the period of 14 to 21 d of age. Moreover, T3 had very highly (p < 0.0001) significant effects on coccidiosis evolution from D14 to D26, as chicks get coccidiosis in cæcum only at day 26 with a load score of “1”. In addition, the lowest (p < 0.01) macroscopic intestinal lesion score was observed in T3 group at day 14 (S = 0,75). For histological analysis, T2 (50g of β-xylanase and β-glucanase mix/ton) and T6 (100 g of a mixture of xylanase, cellulase, β–glucanase, mannanase, α-galactosidase/ton) presented the lowest (p < 0.001) total ISI score. Therefore, we conclude that supplementation of 50 g of a mixture of β-xylanase and β-glucanase per ton of feed improves growth performances, reduces coccidiosis loads and decreases macroscopic and microscopic lesions in broiler chickens.

 

Keywords: NSPase; GPA; Broiler; Growth Performance; Lesion; Coccidiosis

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Citation: Sophia DERQAOUI., et al. “Effects of NSPase Supplementation on Growth Performances, Histological and Macroscopic Lesions and Coccidiosis Load of Broiler Chickens”. EC Veterinary Science 7.5 (2022): 08-19.

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