Research Article
Volume 7 Issue 1 - 2022
A New Frog Species of the Genus Glandirana from Southeastern Kyushu, Japan (Anura Ranidae)
Masahisa Nakamura1,3*, Yoriko Nakamura2, Akira Oike3, Koji Tojo4, Tomoya Suzuki4 and Etsuro Ito3
1Waseda Research Institute for Science and Engineering, Waseda University, Nishi-Waseda, Tokyo, Japan
2Department of Science Education, Faculty of Education, Ehime University, Bunkyo-cho, Matsuyama, Japan
3Department of Biology, Faculty of Education and Integrated Arts and Sciences, Waseda University, Tokyo, Japan
4Department of Biology, Faculty of Science, Shinshu University, Matsumoto, Japan
*Corresponding Author: Masahisa Nakamura, Waseda Research Institute for Science and Engineering, Waseda University, Nishi-Waseda, Tokyo, Japan.
Received: November 29, 2021; Published: December 29, 2021


Glandirana rugosa, long thought to be a single species living in Japan, is separated phylogenetically into four local groups, designated G. rugosa-West, -North, -East, and -Central. Based on genetic identity values of the complete mitogenome sequences of the four groups, we propose that G. rugosa-West represents G. rugosa sensu stricto (s.str.) and G. rugosa-East and -Central should have a different species name. G. rugosa-North may be separated from G. rugosa-West as a new species as well. Recently we have identified a phylogenetically distinct new group of G. rugosa in southeastern Kyushu of Japan, designated se-Kyushu. On a phylogenetic tree, se-Kyushu is clustered differently from G. rugosa-West, although the two populations inhabit parapatrically in Kyushu. We observed significant differences in eye distance (ED) and distance between tympanum and eye (T-ED) and advertisement calls between se-Kyushu and G. rugosa-West. Genetic identity of the mitogenomes between the two populations was much lower than the range of intraspecific genetic identity in the Ranidae frogs. In addition, we observed lower survival rates of embryos and tadpoles of the first reciprocal combination, when the two populations were hybridized. Thus, we concluded that se-Kyushu is a new species in the genus Glandirana.

Keywords: Glandirana nakamurai sp. nov.; Mitochondrial DNA Phylogeny; Morphometry; New Species; Southeastern Kyushu


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