Research Article
Volume 5 Issue 10 - 2020
Immunomodulatin Effect of Inactivated Parapox Virus (Zylexis) on Sheep Crop Vaccinated with Inactivated RVF Vaccine
Ebtsam EZ Kotb1, AbulMagd DM2* and Darwish MD3
1Animal Reproduction Research Institute, Giza, Egypt
2Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Egypt
3Central Laboratory for Evaluation of Veterinary Biologics (CLEVB), Abbasia, Cairo, Egypt
*Corresponding Author: AbulMagd DM, Veterinary Serum and Vaccine Research Institute (VSVRI), Abbasia, Cairo, Egypt.
Received: August 21, 2020; Published: September 24, 2020




Abstract

Rift Valley Fever (RVF) is a mosquito-borne zoonotic disease that presents a substantial threat to human and public health. It is caused by Rift Valley fever Phlebovirus (RVFV), which belongs to the genus Phlebovirus and the family Bunyaviridaes. Both wide distribution of the vectors in non-endemic areas and the climatic change, cause great threat of RVFV spreading.

So, the present study was used zylexis as immunomodulatory factor to increase the immune response of sheep vaccinated with inactivated RVFV vaccine. Twenty sheep were divided into four groups (five animals/group) where the first group was inoculated with Zylexis (2 ml/sheep) two days before vaccination with the field dose 1 ml, (s/c) of inactivated RVF vaccine; the second group was inoculated with Zylexis simultaneously with the vaccine; the third sheep ‘group was inoculated with the vaccine only while the fourth group was kept as non-vaccinated and non-inoculated control. Blood samples were collected on heparin as anticoagulant for evaluation of cellular immune response, while serum samples had been collected for monitoring the humeral immune response using SNT and ELISA. The obtained results demonstrated that sheep inoculated with zylexis with the inactivated RVF vaccine give more humoral and cellular immune response than those inoculated with zylexis two days before vaccination and those inoculated with the vaccine only. These findings revealed that zylexis acts as an immune stimulator in sheep vaccinated with the inactivated RVF vaccine.

Keywords: RVF; SNT; ELISA; Zylexis

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Citation: AbulMagd DM., et al. ““Immunomodulatin Effect of Inactivated Parapox Virus (Zylexis) on Sheep Crop Vaccinated with Inactivated RVF Vaccine”. EC Veterinary Science 5.10 (2020): 54-64.

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