Research Article
Volume 5 Issue 9 - 2020
Total Protein Concentrations in Brain Regions and Testicular/Tubal Fluids of Rabbits Fed Cassava Peel Meal as Replacement for Maize
Evans Emem Iboro1, Ozung Pascal Ogar2* and Kennedy Oluwatosin Ohotuowo Oluwamoroti2
1Department of Animal Science, Akwa Ibom State University, Obio Akpa Campus, Uyo, Akwa Ibom, Nigeria
2Department of Animal Science, Faculty of Agriculture, Forestry and WRM, University of Calabar, Calabar, Nigeria
*Corresponding Author: Ozung Pascal Ogar, Department of Animal Science, Faculty of Agriculture, Forestry and WRM, University of Calabar, Calabar, Nigeria.
Received: July 20, 2020; Published: August 08, 2020


Cheaper unconventional feed materials whose metabolizable energy is similar or closed to maize could be used in animal diets. This study investigated the effect of replacing maize with cassava peel meal (CPM) on the total protein concentrations in the brain regions and testicular/tubal fluids of rabbits. Sixty crossed bred weaned rabbits with an average initial bodyweight of 559.58 ± 0.42g/rabbit were used. The animals were assigned to experimental diets using a completely randomised design. Five experimental diets were formulated with ratios of 100:0, 75:25, 50:50, 25:75 and 0:100 maize: sun dried CPM for T1, T2, T3, T4 and T5, respectively. At the end of the feeding trial, six rabbits per treatment were sacrificed by stunning and decapitation. The heads were severed and immediately dissected for brain harvest. The various brain regions were separately homogenized. The testes and epididymis of the bucks as well as segments of the female reproductive tract were also homogenised and total protein concentrations determined by the biuret method. Results showed that the total protein concentrations in all the brain regions were significantly (P < 0.05) affected by dietary treatments except the medulla oblongata. The protein concentration decreased as the level of CPM replacement increases with 100% level recording the least values. The protein concentration in the testicular/tubal fluids recorded a fluctuating trend that could not be ascribed to diets. The study concluded that cassava peel meal could serve as a better substitute for maize up to 75% without any serious adverse effects on the brain and reproductive organs of rabbits.

Keywords:Brain; Cassava Peel; Protein; Rabbits; Reproduction


  1. Attah S., et al. “Effect of replacement of rice offal with graded levels of melon (Citrullus vulgaris) seed offal on performance of growing rabbits”. The Nigerian Society for Animal Production 38.2 (2011): 67-73.
  2. FAO. “Food and Agriculture Organization of the United Nations. The state of food and Agriculture”. Rome, Italy (2013).
  3. Taiwo AA., et al. “Performance and nutrient digestibility of weaned rabbits fed forages supplemented with concentrate”. The Nigerian Society for Animal Production 32.1 (2005): 74-78.
  4. Oyedeji OJ., et al. “Performance and carcass characteristics of weaned rabbits fed varying levels of unfermented cocoa pod husk meal (CPHM). Proceeding of the 36th Conference of Nigerian Society for Animal Production, 13th -16th March, University of Abuja Nigeria (2011): 758-761.
  5. Aduku AO and Olukosi JO. “Rabbit Management in the Tropics: production, processing, utilization, marketing, economics, practical training, research and prospects Living Books GU publication, Abuja (1990).
  6. Ojebiyi OO., et al. “Reproductive potentials of rabbit does fed diets containing processed cassava peel/ blood meal mixture”. International Journal of Livestock Production 4.4 (2013): 42-48.
  7. FAO. “Food and Agriculture Organization of the United Nations. Good practices for industry- implementation, the codex Alimentarius Code of practice on good animal feeding”. FAO Animal Production and Health Manual, Rome (2010): 9.
  8. Agunbiade JA., et al. “Performance characteristics of weaner rabbits on cassava peels based balanced diets”. The Nigerian Society for Animal Production 29 (2002): 171-175.
  9. McCalla AF. “World food prices: causes and consequences”. Canada Journal of Agricultural Economics 57 (2009): 23-34.
  10. Nwokoro SO., et al. “Effect of replacement of maize with Cassava peel on cockerels’ diet on performance and carcass characteristics”. Tropical Animal Health and Production 37.6 (2005): 495-501.
  11. Omole AJ., et al. “Performance characteristics of weaned rabbits fed graded levels of dried cassava peel fortified with soy corn residue basal diet”. Journal of Agriculture and Environment 3.1 (2005): 27-32.
  12. Anderson MD., et al. “Cytochromes P-450 from cassava (Manihot esculanta crantz) catalysing the first steps in the biosynthesis of the cyanogenic glucosides, linamarin and lotaustralin”. Journal of Biological Chemistry 275 (2000): 1966-1975.
  13. Google Earth. GeoNames geographical database, courtesy of Google Earth. Traveling luck for Calabar, Cross River State, Nigeria. Time Zone, Latitude, Longitude, elevation and map satellite image (2019).
  14. AOAC. Association of Official Analytical Chemists. Official methods of analysis, 16th edition, Washington, DC 1 (2000): 69-90.
  15. Bitto II., et al. “Changes in protein concentration of testicular spermatozoa and fluid during sperm maturation in male rabbits fed kapok (Ceiba pentandra) seed meal. Proceeding of the 33rd Annual conference of the Nigerian Society for Animal Production, held at the University of Nigeria, Nsukka, Nigeria (2008).
  16. Egbunike GN. “Regional distribution of acetyl cholinesterase activity in the brain and hypophyses of crossbred European boars reared in the humid tropics”. Acta Anaesthesiologica 110 (1981): 248-252.
  17. Gbore FA and Egbunike GN. “Total protein concentrations in brain regions and hypophyses of pubertal boars fed dietary fumonism B1. Proceeding of the 37th Annual Conference of Nigerian Society of Animal Production (NSAP, held at the College of Animal Science, University of Agriculture, Makurdi (2012): 144-147.
  18. Egbunike GN and Adegunle SA. “Regional variation in protein and alkaline phosphate content in the genital tract fluid of porcine females”. American Journal of Veterinary Medicine 27 (1980): 319-329.
  19. Steel RGD and Torrie JH. “Principles and Procedures of Statistics. A Biometric Approach”. 2nd edition, McGraw Hill Book Co. Inc. New York, USA (1980): 481.
  20. Bitto II. “Total protein and cholesterol concentrations in brain regions of male rabbits fed pawpaw peel meal based diets”. African Journal of Biomedical Research 11 (2008): 73-78.
  21. Bitto II and Egbunike GN. “Seasonal variations in the morphometric characteristics of the pubertal West African dwarf buck in its native tropical environment”. International Journal of Morphology 24 (2006): 637-642.
  22. Ozung PO., et al. “The potentials of small ruminant production in Cross River Rain Forest Zone of Nigeria: A review”. Continental Journal of Animal and Veterinary Research 3.1 (2011): 33-37.
Citation: Ozung Pascal Ogar., et al.“Total Protein Concentrations in Brain Regions and Testicular/Tubal Fluids of Rabbits Fed Cassava Peel Meal as Replacement for Maize”. EC Veterinary Science 5.9 (2020): 40-46.

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