Research Article
Volume 5 Issue 9 - 2020
A Review on Various Approaches to the Identification of Listeriae
Mouloua A1*, Seklaoui N1, Mezai G2, Kerrachi I2, Bouiba L2, Edaikra N2, Benikhlef R2 and Harrat Z2
1Assistant Professor, Determent of Veterinary Public Health and Epidemiology, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK HP Agricultural University, Palampur, HP, India
2Phd Scholar, School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India
3Assistant Professor, School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India
*Corresponding Author: Asima Zehra, Phd Scholar, School of Public Health and Zoonoses, Guru Angad Dev Veterinary and Animal Sciences University, Punjab, India.
Received: July 14, 2020; Published: August 29, 2020




Abstract

Historically, for a considerable period, Listeria spp. remained unnoticed as a major food pathogen which can be credited to its challenging isolation from food or other samples. But now L. monocytogenes is an established food-borne pathogen of public health significance and is widely present in food, environmental and clinical samples. The very first step for the isolation of the organisms are culture methods that characterise the organisms based on the morphology of the colony, fermentation of sugar and their haemolytic effects. These are the gold standards; but they are time consuming and may not be suitable for testing of perishable foods. As a result, more rapid and sensitive serological (ELISA) and molecular (real time PCR or DNA hybridization) tests were developed. These tests not only provide a measure of cell viability but they can also be used for quantitative analysis. In addition, a variety of tests can be used for sub-species characterizations, which are particularly useful in epidemiological investigations. Early typing methods differentiated isolates based on phenotypic markers, such as MLEE, phage typing and serotyping. These phenotypic typing methods are being replaced by molecular tests, which reflect genetic relationships between isolates and are more accurate. These new methods are currently mainly used in research but their considerable potential for routine testing in the future cannot be overlooked.

Keywords: L. monocytogenes; Serological Assays; Molecular Assays

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Citation: Asima Zehra., et al. “A Review on Various Approaches to the Identification of Listeriae”. EC Veterinary Science 5.9 (2020): 162.

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