Research Article
Volume 8 Issue 1 - 2020
Studies on Formulation Development, In-vitro and Ex-vivo Characterization of Ion-Activated Ophthalmic In-situ Gel Containing Moxifloxacin Nanoparticles
Nagaraju R1*, Vanaja CH1 and Abdul Wahid Ambekar2
1Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women’s University), Tirupati, Andhra Pradesh, India
2Department of Pharmaceutics, Dr. Vithalrao Vikhe Patil Foundation’s College of Pharmacy, Vadgaon Gupta (Vilad Ghat) PO MIDC, Ahmednagar, Maharashtra, India
*Corresponding Author: Nagaraju R, Institute of Pharmaceutical Technology, Sri Padmavati Mahila Visvavidyalayam (Women’s University), Tirupati, Andhra Pradesh, India.
Received: November 22, 2019; Published: December 06, 2019




Abstract

Development of ion-activated ophthalmic in-situ gel containing moxifloxacin nanoparticles for better control of the release of the drug, minimize the dosing frequency and to improve the efficacy. Moxifloxacin nanoparticles were prepared by anti-solvent evaporation technique by using polyvinyl alcohol (PVA), Poloxomer 407 and Eudragit® RSPO. Prepared nanoparticles were loaded into the ion-activated in-situ gelling system (Sodium alginate) which undergoes phase transition from sol- gel in the presence of mono or divalent ions in the physiological conditions. The prepared in-situ gel formulations were evaluated for clarity, pH, gelling capacity, rheological properties, in-vitro drug release studies, ex-vivo corneal permeation studies, anti-microbial activity and histopathological studies. The PF3 formulation showed good gelling capacity with high % drug content. Rheological properties were acceptable for PF3 formulation when compared with the other formulations and in-vitro, ex-vivo release studies showed that the drug release was sustained and 65%, 62.04% was released in 4 hrs. Anti-microbial activity indicates that moxifloxacin nanoparticle loaded ophthalmic in-situ gel showed greater zone of inhibition when compared with the pure drug. Histopathological study revealed that there was no morphological difference between the formulations treated cornea and the healthy cornea. Hence can be concluded that formulation PF3 developed in the present study can be the successful replacement of conventional ophthalmic solutions.

Keywords: Moxifloxacin; PVA; Eudragit® RSPO; In-situ Gel; Anti-microbial Activity; Histopathological Study

References

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Citation: Nagaraju R., et al. “Studies on Formulation Development, In-vitro and Ex-vivo Characterization of Ion-Activated Ophthalmic In-situ Gel Containing Moxifloxacin Nanoparticles". EC Pulmonology and Respiratory Medicine 8.1 (2020): 01-14.

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