Research Article
Volume 8 Issue 2 - 2020
HPLC Standardization of Herbal Drugs and Evaluation of their Antimicrobial Properties: Studies of Leaves and Flowers of Senna italica Mill. Grown in Benin (Western Africa)
Alidehou Jerrold Agbankpe1*, Aurel C Allabi2,3, Joachim Hode2,3, Yolande Sissinto Savi de Tove4, Adrien N Noudjiegbe2,3, Tamègnon Victorien Dougnon1, Boris Brice Legba1 and Honore Sourou Bankole1
1Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin
2Faculty of Health Sciences, Laboratory of Pharmacology and Toxicology of Faculty of Health Sciences, University of Abomey-Calavi, Benin
3Beninese Center of Scientific Research and Innovation, National Laboratory of Narcotic and Toxicology, Benin
4Teaching and Research Unit in Parasitology-Mycology, Faculty of Health Sciences, University of Abomey-Calavi, Benin
*Corresponding Author: Alidehou Jerrold Agbankpe, Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Benin.
Received: December 09, 2019; Published: January 18, 2020




Abstract

This study aims to evaluate the phytochemistry and antimicrobial activities of the leaves and flowers of Senna italica Mill. 

Qualitative phytochemical screening was done with method based on differential coloration and precipitation. Thin Layer Chromatography was used for characterization of sennosides in aqueous and hydro-ethanolic extract of leaves and flowers of S. italica. Then HPLC was used for quantification of sennosides. The antifungal activity of the extracts was evaluated on Candida albicans and Trichophyton rubrum by incorporation method. Well diffusion technique, coupled with the microdilution determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (CMB) was used for antibacterial testing. 

The results of study showed the presence of large groups of secondary metabolites in the leaves and flowers of S. italica including tannins, galic tannins, flavonoids, steroids and some glycosides. TLC test showed that leaves and flowers of S. italica contain sennosides A, B, C and D. HPLC test showed that the content of the sennosides of flowers is higher than that of the dried leaves in the sun. According to daily dose of sennosides recommended, 20 mg to 30 mg as laxative, this dose can be attained with 0.718 to 0.862g of leaf powder or 1.390 to 1.668g of S. italica flower powder. The extracts have no antifungal activity but the hydroethanolic extracts of both leaves and flowers of S. italica have bactericidal activity on strains of S. aureus ATCC 25923, E. coli ATCC 25922 and E. coli clinical strain. 

This quantification of sennosides is a standardization of the herbal tea of S. italica and will limit these possible side effects. This plant is a good candidate for the development of improved traditional medicine.

Keywords: Senna italica; Sennosides; HPLC; Antifungal and Antibacterial Activity; Benin

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Citation: Alidehou Jerrold Agbankpe., et al. “HPLC Standardization of Herbal Drugs and Evaluation of their Antimicrobial Properties: Studies of Leaves and Flowers of Senna italica Mill. Grown in Benin (Western Africa)”. EC Psychology and Psychiatry 8.2 (2020): 01-17.

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