Research Article
Volume 8 Issue 1 - 2020
Anti-Trypanosomal Activity of Aqueous Leaf Extract of Annona senegalensis against Trypanosoma congolense
Oke Philip Oladele1, Nicholas Nyoyong Nongo1 and Saganuwan Alhaji Saganuwan2*
1Department of Veterinary Parasitology and Entomology, College of Veterinary Medicine, Federal University of Agriculture, Makurdi, Benue State, Nigeria
2Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Federal University of Agriculture, Makurdi, Benue State, Nigeria
*Corresponding Author: Saganuwan Alhaji Saganuwan, Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Federal University of Agriculture, Makurdi, Benue State, Nigeria.
Received: November 15, 2019; Published: December 31, 2019




Abstract

This study investigated in vitro and in vivo anti-trypanosomal activity of cold (25°C) and hot (80°C) aqueous extracts of Annona senegalensis leaf against Trypanosoma congolense. The phytochemical screening showed presence of flavonoids, phlebotannins, resins, and saponins. Hot aqueous extract revealed presence of tannins and glycosides which were absent in cold aqueous extract. But alkaloids were absent in the two extracts. The median lethal doses (LD50) for both cold and hot aqueous leaf extracts were estimated at dose level of > 16,095 mg/kg. The in vitro study revealed inhibition of parasites’ motility at 100 mg/ml concentration of hot aqueous extract 30 minutes post application of the extract while 200 mg/ml concentration of the same extract inhibited parasites’ motility at 50 minutes post application of the extract. Cold aqueous extract inhibited parasites’ motility 50 and 20 minutes post application of 100 mg/ml and 200 mg/ml concentration respectively. In vivo screening of anti-trypanosomal activity showed that both cold and hot aqueous extracts were not effective at the dose level of 161 mg/kg but showed anti-trypanosomal activity at the dose levels of 322 mg/kg and 483 mg/kg body weight respectively. Hence Annona senegalensis may serve as a resource plant for discovery of drug(s) that can be used for the treatment of trypanosomosis caused by Trypanosoma congolense.

Keywords: Anti-trypanosomal Activity; Annona senegalensis; Cold Extract; Hot Extract; Trypanosomosis; Trypanosoma congolense

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Citation: Oke Philip Oladele., et al. “Anti-Trypanosomal Activity of Aqueous Leaf Extract of Annona senegalensis against Trypanosoma congolense”. EC Pharmacology and Toxicology 8.1 (2020): 01-08.

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