Research Article
Volume 9 Issue 3 - 2021
Preliminary Phytochemical Analysis and Peripheral Analgesic Activity Studies of Alpinia malaccensis MeOH Extract
Rashed Reza1, Promita Deb2, Sumona Afrose1 and Mohammed Rahmatullah1*
1Department of Pharmacy, Faculty of Life Sciences, University of Development Alternative, Dhaka, Bangladesh
2Department of Biotechnology and Genetic Engineering, University of Development Alternative, Lalmatia, Dhaka, Bangladesh
*Corresponding Author: Mohammed Rahmatullah, Professor and Dean, Faculty of Life Sciences, Department of Pharmacy, University of Development Alternative, Lalmatia, Dhaka, Bangladesh.
Received: December 28, 2020; Published: February 27, 2021


The analgesic effect of crude methanol extracts of Alpinia malaccensis (Burm.f.) Roscoe (Zingiberaceae) was evaluated in acetic acid-induced pain model experiment in Swiss albino mice. This study was designed to assess the nature of phytochemicals present and determine the peripheral pain relief properties of methanolic extract of aerial parts (MEAMA) and rhizome (MEAMR) of the plant. Both extracts contained alkaloids, flavonoids, steroids, saponins and tannins. In analgesic activity test, the methanol extract at doses of 50, 100, 200 and 400 mg/kg body weight dose-dependently reduced the number of writhing by 31.7, 46.3, 51.2 and 58.5% for MEAMA and 19.5, 41.5, 53.7 and 61.1% for MEAMR, respectively. The standard analgesic drug, aspirin, when administered at doses of 200 and 400 mg/kg body weight reduced writhing by 41.5 and 63.4%, respectively. Compared to control, all the given doses except low dose (50 mg) of rhizome extract of Alpinia malaccensis significantly decreased the pain. Considering present data it can be suggested that the methanol extracts of Alpinia malaccensis have chemical compounds potentially responsible for alleviating pain and might pave the way towards development of a novel analgesic agent in future.


Keywords: Analgesic; Alpinia malaccensis; Phytochemicals; Peripheral Pain; Zingiberaceae


    1. Sahoo S., et al. “Chemical composition, antioxidant and antimicrobial activity of essential oil and extract of Alpinia malaccensis Roscoe (Zingiberaceae)”. International Journal of Pharmacy and Pharmaceutical Sciences7 (2014): 183-188.
    2. Somarathna T., et al. “Antimicrobial activity and phytochemical screening of Alpinia malaccensis (Ran-kiriya) against food-borne bacteria”. Journal of Applied Microbiology5 (2018): 1276-1285.
    3. Lakhan SE., et al. “Zingiberaceae extracts for pain: a systematic review and meta-analysis”. Nutrition Journal 14 (2015): 50.
    4. Hossain AI., et al. “A preliminary evaluation of antihyperglycemic and analgesic activity of Alternanthera sessilis aerial parts”. BMC Complementary and Alternative Medicine 14 (2014): 169-173.
    5. Kumar C., et al. “Phytochemical properties, total antioxidant status of acetone and methanol extract of Terminalia arjuna bark and its hypoglycemic effect on Type-II diabetic albino rats”. Journal of Pharmacognosy and Phytochemistry 2.1 (2013): 199-208.
    6. Koster R and erson M De Beer EJ. “Acetic acid for analgesic screening”. Federation Proceedings 18 (1959): 412-417.
    7. Hussein RA and El-Anssary A. “Plants secondary metabolites: The key drivers of the pharmacological actions of medicinal plants”. In Herbal Medicine. Intech Open: Giza, Egypt (2019): 11-30.
    8. Hasan MM., et al. “Analgesic and antiinflammatory activities of leaf extract of Mallotus repandus (Willd.). Muell. Arg”. BioMed Research International (2014): 1-7.
    9. Shah SMM., et al. “Antioxidant, total phenolic contents and antinociceptive potential of Teucrium stocksianum methanolic extract in different animal models”. BMC Complementary and Alternative Medicine1 (2014): 181.
    10. Duarte ID., et al. “Participation of the sympathetic system in acetic acid-induced writhing in mice”. Brazilian Journal of Medical and Biological Research2 (1988): 341-343.
    11. Chen YF., et al. “Anti-inflammatory and analgesic activities from roots of Angelica pubescens”. Planta Medica1 (1995): 2-8.
    12. Ahmadiani A., et al. “Anti-nociceptive and anti-inflammatory effects of Eleagnus angustifolia fruit extract”. Journal of Ethnopharmacology1-2 (2000): 287-292.
    13. Ramesh M., et al. “Antinociceptive and anti-inflammatory activity of a flavonoid isolated from Caralluma attenuate”. Journal of Ethnopharmacology1 (1998): 63-66.
    14. Adedapo A., et al. “Phytochemistry, anti-inflammatory and analgesic activities of the aqueous leaf extract of Lagenaria breviflora (Cucurbitaceae) in laboratory animals”. Revista de Biología Tropical1 (2013): 281-290.
    15. Hassan HS., et al. “Anti-inflammatory activity of crude saponin extracts from five Nigerian medicinal plants”. African Journal of Traditional, Complementary and Alternative Medicines2 (2012): 250-255.
    16. Das BK., et al. “Phytochemical screening and evaluation of analgesic activity of Oroxylum indicum”. Indian Journal of Pharmaceutical Sciences6 (2014): 571-575.
    17. Mali AA., et al. “Antiinfammatory and analgesic activities of ethyl acetate and petroleum ether fractions of Cassia auriculata Leaves”. Oriental Pharmacy and Experimental Medicine 13.3 (2013): 191-197.
    18. Raj NK., et al. “Bioflavonoids classification, pharmacological, biochemical effects and therapeutic potential”. Indian Journal of Pharmacology 33 (2001): 2-16.
    19. Lopes K., et al. “Chemical composition, toxicity, antinociceptive and anti-inflammatory activity of dry aqueous extract of Varronia multispicata (Cham.) Borhidi (Cordiaceae) leaves”. Frontiers in Pharmacology 10 (2019): 1376.
    20. , et al. “Ethnomedical uses of Zingiberaceous plants of Northeast India”. Journal of Ethnopharmacology 132.1 (2010): 286-296.
Citation: Mohammed Rahmatullah., et al. “Preliminary Phytochemical Analysis and Peripheral Analgesic Activity Studies of Alpinia malaccensis MeOH Extract”. EC Pharmacology and Toxicology 9.3 (2021): 64-69.

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