Research Article
Volume 9 Issue 3 - 2021
Amelioration of Neurobehavioral and Biochemical Changes by Withania somnifera Root Extract During Type 2 Diabetes Mellitus Induced Cognitive Deficit in Rats
Anshul Tanwar1, Kavita Gulati1* and Arunabha Ray2*
1Department of Pharmacology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India
2Department of Pharmacology, Hamdard Institute of Medical Sciences and Research, Jamia Hamdard, Delhi, India
*Corresponding Author: Kavita Gulati, Professor, Department of Pharmacology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India.
Received: January 31, 2021; Published: February 27, 2021


Objective: To investigate the effects of Withania somnifera root extract (WS) on type 2 diabetes mellitus induced cognitive deficit.

Methods: Type 2 diabetes mellitus (T2DM) was induced in rats by feeding them with high fat diet (HFD) for 8 weeks followed by intraperitoneal administration of Streptozotocin (STZ; 35 mg/kg). After four weeks, the fasting blood glucose, serum triglycerides, serum cholesterol and serum insulin levels were analyzed. After the confirmation of diabetes, WS was administered (two doses) for 8 weeks to the diabetic rats. After completion of the treatment animals were tested for memory deficits by Morris water maze (MWM) test and blood sample were collected for biochemical analysis. Glucose tolerance was determined by oral glucose tolerance test (OGTT) and insulin resistance by HOMA-IR models and all the results were compared with standard drug Pioglitazone (PZ).

Results: Significant elevation in fasting blood glucose, triglyceride, cholesterol and insulin levels were observed in HFD-STZ induced diabetic rats as compared to control animals and treatment with WS for 8 weeks significantly lowered these levels. In MWM test, diabetic rats spent significantly less time in target quadrant (TSTQ), an indicator of cognitive deficit for spatial learning, as compared to control animals. The animals treated with WS spent significantly more time in TSTQ when compared with diabetic group.

Conclusion: The data suggested that 8 weeks of treatment with Withania somnifera lowered the metabolic parameters of type 2 diabetes when compared with diabetic group. The reversal of biochemical markers was accompanied with significant improvement in memory retention and reduction in insulin resistance in rats with T2DM induced cognitive deficit. Thus, it can be concluded that Withania somnifera has therapeutic potential and can be explored in the management of T2DM induced Alzheimer’s disease.

Keywords: Metabolic Syndrome; Type 2 Diabetes; Insulin Resistance; Cognition; Neurobehavioral Studies; High-fat Diet; Withania somnifera


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Citation: Kavita Gulati., et al. “Amelioration of Neurobehavioral and Biochemical Changes by Withania somnifera Root Extract During Type 2 Diabetes Mellitus Induced Cognitive Deficit in Rats”. EC Pharmacology and Toxicology 9.3 (2021): 45-58.

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