Research Article
Volume 9 Issue 2 - 2021
Phenotypic and Molecular Detection of Invasive Pulmonary Aspergillosis from Inpatients Children with Hematologic Cancers
Sedigheh Rafiei Tabatabaei1, Mehdi Shirdoust1, Samin Alavi2, Behnaz Lajevardi1, Farideh Shiva1, Mohamad Ali Gudarzi1, Seyedeh Mahsan Hoseini-Alfatemi1* and Somayeh Sharifynia3*
1Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2Pediatric Congenital Hematologic Disorders Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran
33Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
*Corresponding Author: Seyedeh Mahsan Hoseini-Alfatemi, Pediatric Infections Research Center, Research Institute for Children’s Health, Shahid Beheshti University of Medical Sciences, Tehran, Iran and Somayeh Sharifynia, Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Received: September 05, 2021; Published:Published: January 30, 2021




Abstract

Objective: Invasive pulmonary aspergillosis (IPA) is a growing concern in immunocompromised children. Data from Middle East regarding burden of children’s Aspergillus infections are scant. This study aimed to update the frequency and antifungal susceptibility profile of Aspergillus isolates obtained from children with malignant underlying diseases.

Materials/Patients/Methods: A total of 75 episodes from 60 patients with clinical presentation suggestive of IPA were recruited between January 2013 to December 2016, and clinical data were retrospectively collected. In vitro susceptibility was determined by E test method using Clinical and Laboratory Standards Institute guidelines.

Result: According to EORTC/MSG criteria, a total of 75 episodes, 16 (21.3%) and 59 (78.7%) cases with probable and possible IPA were identified, respectively. Aspergillus flavus was isolated in 7neutropenic patients showing and showed reduced susceptibility to amphotericin B. However, all isolates demonstrated susceptible profile to caspofungin (≤ 0.25 µg/ml), posaconazole (≤ 0.5 µg/ml) and voriconazole (≤ 2 µg/ml).

Conclusion: The Aspergillus species causing IPA in children with malignancies differed from those in Western countries. Our results provide useful information on local epidemiology of IPA and selection of proper empirical antifungal agent.

 

Keywords: Invasive Pulmonary Aspergillosis; Children, Aspergillus flavus; Neutropenia; Iran

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Citation: Seyedeh Mahsan Hoseini-Alfatemi and Somayeh Sharifynia., et al. “Phenotypic and Molecular Detection of Invasive Pulmonary Aspergillosis from Inpatients Children with Hematologic Cancers”. EC Pharmacology and Toxicology 9.2 (2021): 51-59.

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