Research Article
Volume 9 Issue 2 - 2021
The Gasotransmitter Mechanism of the Erythropoietin Protection Against Hepatic Reperfusion Injuries: The Role of Hydrogen Sulfide
Michail Khodosovsky*
Department of Pathophysiology, Grodno State Medical University, Belarus
*Corresponding Author: Michail Khodosovsky, Department of Pathophysiology, Grodno State Medical University, Belarus.
Received: August 31, 2020; Published: January 27, 2021


Introduction: The erythropoietin administration can be a new method for correction of hepatic reperfusion injuries, which are an actual problem of current surgery and transplantation. The purpose of this work was to investigate the role of hydrogen sulfide in the erythropoietin protective effect during hepatic ischemia-reperfusion (HIR) in rats.

Methods: 40 male Wistar rats were divided into 4 groups: 1st (n = 10) - control; 2nd (n = 10) - HIR: hepatic ischemia (30 min, m. Pringle) and reperfusion (120 min); in 3rd group (n = 10) erythropoietin (INTAS, 1000 IU/kg) was admin 30 min before HIR; in 4th group (n = 10) rats were handled like in 3rd group, but inhibitor of cystathionine-γ-lyase (DL-propargylglycine, 50 mg/kg) was added 1hr before HIR. The parameters of pro/antioxidant balance (conjugated diens, malondialdehyde, reduced glutathione, catalase activity and ect.) were detected in blood and liver at the end of experiments.

Results: It was found that erythropoietin significantly decreased the level of lipid peroxidation products, improved parameters of antioxidant system in blood and liver at the end of reperfusion. The inhibition of endogenous hydrogen sulfide synthesis by DL-propargylglycine significantly reduced this protective effect of erythropoietin.

Conclusion: The protective effect of erythropoietin during hepatic ischemia-reperfusion is largely mediated by gasotransmitter properties of hydrogen sulfide.

Keywords: Hydrogen Sulfide; Erythropoietin; Reperfusion; Liver; Prooxidant-Antioxidant Status; Rats


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Citation: Michail Khodosovsky. “The Gasotransmitter Mechanism of the Erythropoietin Protection Against Hepatic Reperfusion Injuries: The Role of Hydrogen Sulfide”. EC Pharmacology and Toxicology 9.2 (2021): 11-16.

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