Abstract

Fluoride in excess is a toxic chemical to the kidney function. Studies implicated that aggravating oxidative stress and inhibiting body antioxidative systems is the main route of damage. Moringa stenopetala is rich in antioxidants and has a potent anti-oxidative effect. The study aimed to determine the effect of sub-chronic exposure to fluoride and the effect of Moringa stenopetala crude extract on body weight and kidney function. Randomized control study was conducted using 2 months old, 24 adult Swiss Albino mice. All animals were allowed free access to standard pellet diet ad libitum and distilled water. The mice were randomly divided into six groups of four animals, taking different doses of fluoride and the crude extract. After 3 months of exposure, the mice were anesthetized and sacrificed under anesthesia for sample collection. There was a statistically significant difference in renal function test (plasma urea) between the treated and the control groups in high dose fluoride group. Independent t-test yields no significant difference body mass and organ mass between fluorotic groups without Moringa stenopetala and fluorotic with Moringa stenopetala groups (P = 0.318). Though, there was some evidence indicating Moringa stenopetala might provide some protection, further studies are necessary to explore the role of Moringa stenopetala, as no significant difference was observed between groups with and without the plant extract in this study.

Keywords: Fluorosis; Sodium Fluoride; Renal Function; Moringa stenopetala; Weight; Mice

References

1. Kloos H and Haimanot RT. “Distribution of fluoride and fluorosis in Ethiopia and prospects for control”. Tropical Medicine and International Health 4 (1999): 355-364.
2. Tamer MN. “Fluorosis☆”. In Reference Module in Earth Systems and Environmental Sciences (2018).
3. Ajiboye AS., et al. “Supporting community water fluoridation”. Dental Abstracts 64 (2019): 157-158.
4. Viswanathan G., et al. “Determining the optimal fluoride concentration in drinking water for fluoride endemic regions in South India”. Science of the Total Environment 407 (2009): 5298-5307.
5. Everett ET. “Fluoride’s Effects on the Formation of Teeth and Bones, and the Influence of Genetics”. Journal of Dental Research 90 (2011): 552-560.
6. Dhar V and Bhatnagar M. “Physiology and toxicity of fluoride”. Indian Journal of Dental Research 20 (2009): 350.
7. Zhavoronkov AA. “[Non-skeletal forms of fluorosis]”. Arkhiv Patologii 39 (1977): 83-91.
8. Cittanova ML., et al. “Fluoride Ion Toxicity in Human Kidney Collecting Duct Cells”. Anesthesiology | American Society of Anesthesiologists 84 (1996): 428-435.
9. Santoyo-Sanchez MP., et al. “Effects of Acute Sodium Fluoride Exposure on Kidney Function, Water Homeostasis, and Renal Handling of Calcium and Inorganic Phosphate”. Biological Trace Element Research 152 (2013): 367-372.
10. Yang K., et al. “Fluoride in Drinking Water: Effect on Liver and Kidney Function☆”. In Reference Module in Earth Systems and Environmental Sciences (2018).
11. Barbier O., et al. “Molecular mechanisms of fluoride toxicity”. Chemico-Biological Interactions 188 (2010): 319-333.
12. Sun Z., et al. “Fluoride-induced apoptosis and gene expression profiling in mice sperm in vivo”. Archives of Toxicology 85 (2011): 1441-1452.
13. Yang S., et al. “Sodium fluoride induces apoptosis and alters bcl-2 family protein expression in MC3T3-E1 osteoblastic cells”. Biochemical and Biophysical Research Communications 410 (2011): 910-915.
14. K Yisehak., et al. “Contribution of Moringa (Moringa stenopetala, Bac.), a Highly Nutritious Vegetable Tree, for Food Security in South Ethiopia: A Review”. Asian Journal of Applied Sciences 4 (2011): 477-488.
15. Seid M. “Medicinal and Dietary Role of Moringa stenopetala (Bak. f.) Cuf. in South Ethiopia: A Review”. African Journal of Agricultural Science and Technology 1 (2013): 1-16.
16. Habtemariam S and Varghese GK. “Extractability of Rutin in Herbal Tea Preparations of Moringa stenopetala Leaves”. Beverages (2015): 169-182.
17. Libsu TT. “Assessment of antioxidant potential of Moringa stenopetala leaf extract”. The Ethiopian Journal of Science and Technology 2 (2014): 93-104.
18. Khalafalla M and Abdellatef E. “Active principle from Moringa oleifera Lam leaves effective against two leukemias and a hepatocarcinoma”. African Journal of Biotechnology 9 (2010): 8467-8471.
19. Cruz-Vega D., et al. “Review of pharmacological effects of Glycyrrhiza radix and its bioactive compounds”. Zhongguo Zhong Yao Za Zhi Zhongguo Zhongyao Zazhi China 22 (2009): 557-559.
20. Charan J and Kantharia ND. “How to calculate sample size in animal studies?” Clinical Journal of Pharmacology 4 (2013): 303-306.
21. Samal UN. “Effect of fluoride pollutants on growth of certain freshwater fishes”. Journal of Ecology of Environment 12 (1994): 218-220.
22. Buttner W and Karle E. “Chronic toxicity and retention of fluoride in the unilaterally nephrectomized rat”. Caries Research 8 (1984): 357-367.
23. Zhan X., et al. “Effects of fluorosis on lipid peroxidation and antioxidant systems in young pigs”. Fluoride 38 (2005) 157-161.
24. Tsunoda M., et al. “Changes in fluoride levels in the liver, kidney, and brain and in neurotransmitters of mice after subacute administration of fluoride”. Fluoride 38 (2005): 284-292.
25. Vandana V Patil and Varsha T Dhurvey. “Exposure To Sodium Fluoride Affects Thyroid Follicular Cells In Albino Rats”. International Journal of Plant, Animal and Environmental Sciences 5 (2015): 56-61.
26. Selim AO., et al. “Effect of sodium fluoride on the thyroid gland of growing male albino rats : histological and biochemical study”. Egyptian Journal of Histology 35 (2012): 470-482.
27. Kessabi M., et al. “Experimental acute sodium fluoride poisoning in the sheep: Renal, hepatic and metabolic effects”. Fundamental and Applied Toxicology 5 (1985): 1025-1033.
28. Momin R. “Fluoride and aluminium induced toxicity in mice epididymis and its mitigation by vitamin c”. fluoride 38 (2005): 115-121.
29. Sharma JD. “Prevalence of neurological manifestations in a human population exposed to fluoride in drinking water”. Fluoride 142 (2009): 127-132.
30. Rango T., et al. “Groundwater quality and its health impact: An assessment of dental fluorosis in rural inhabitants of the Main Ethiopian Rift”. Environment International 43 (2012): 37-47.
31. Nagarajappa R., et al. “Comparative Assessment of Intelligence Quotient among Child- ren Living in High and Low Fluoride Areas of Kutch, India- a Pilot Study”. Iranian Journal of Public Health 42 (2013): 813-818.
32. Grandjean B. “Rolling Revision of the WHO Guidelines for Drinking-Water Quality Draft for review and comments (Not for citation) Water Requirements, Impinging Factors, and Recommended Intakes”. Calderon (2004): 1-9.
33. Ravula S., et al. “Effect of fluoride on reactive oxygen species and bone metabolism in postmenopausal women”. Fluoride 45 (2012): 108-115.
34. Suska M. “Energy Metabolism of Erythrocytes in Lambs Chronically Exposed to Fluorine Compounds Fluorine is known for its exceptional biological activity (Guminska 1994). Chronic environmental exposure of animals to fluorine compounds results in bone fluorosis and”. Acta Veterinaria Brno 71 (2002): 313-317.
35. Kuang P., et al. “Suppressive effects of sodium fluoride on cultured splenic lymphocyte proliferation in mice”. Oncotarget 7 (2016): 1-11.
36. Khan I and Ranga A. “Biochemical changes in sodium fluoride induced kidney of Swiss albino mice and its amelioration by ascorbic acid”. International Journal of Advance Pharmaceutical and Biological ... Pharmacology and Toxicology, Medicinal Chemistry 3 (2014): 948-956.
37. El-Demerdash M., et al. “Possible protective role of grape seed proanthocyanidin on fluoride induced testicular toxicity in adult and young albino rats”. Mansoura Journal of Forensic Medicine and Clinical Toxicology 16 (2008): 89-107.
38. Buzalaf MAR and Whitford GM. “Fluoride Metabolism”. Fluoride and the Oral Environment 22 (2011): 20-36.
39. Whitford GM. “Fluoride Metabolism and Excretion in Children”. Journal of Public Health Dentistry 59 (1999): 224-228.
40. Mittal M and Flora SJS. “Effects of individual and combined exposure to sodium arsenite and sodium fluoride on tissue oxidative stress, arsenic and fluoride levels in male mice”. Chemico-Biological Interactions 162 (2006): 128-139.
41. Shashi A., et al. “Toxic effects of fluoride on rabbit kidney”. Fluoride 35 (2002): 38-50.
42. Birkner E., et al. “Disturbance of protein metabolism in rats after acute poisoning with sodium fluoride”. Fluoride 33 (2000): 182-186.
43. Dote T., et al. “Toxicokinetics of intravenous fluoride in rats with renal damage caused by high-dose fluoride exposure”. International Archives of Occupational and Environmental Health 73 (2000): S90-S92.
44. Liu JL., et al. “[The dose-effect relationship of water fluoride levels and renal damage in children]”. Wei Sheng Yan Jiu 34 (2005): 287-288.
45. Tebeka T and Libsu S. “Assessment of antioxidant potential of Moringa stenopetala leaf extract”. The Ethiopian Journal of Science and Technology 7 (2014): 93-104.