Review Article
Volume 8 Issue 6 - 2020
Electron Scavenging Not Free Radical Scavenging by Fullerene Materials Protects against Mitochondrial Oxidative Stress in Complex Organisms
Daniel J Bourassa1*, Orien L Tulp1,2,3 and George P Einstein1,2,3
1Einstein Medical Institute, North Palm Beach, FL, USA
2Faculty of Medicine, University of Science Arts and Technology, Olveston, Montserrat, British West Indies
3Faculty of Medicine, University of Health and Humanities, Tortola, British Virgin Islands
*Corresponding Author: Daniel J Bourassa, College of Medicine, University of Science, Arts and Technology, Arvada, CO, USA.
Received: March 04, 2020; Published: May 14, 2020


Fullerene materials are often reported to be excellent antioxidants in cells. Usually, direct free radical scavenging mechanisms, as seen in polymer and food industry research, is the proposed antioxidant mechanism. However, free radical scavenging by antioxidants in vivo is kinetically unlikely and conflicts with the molecular properties of fullerene materials. Pristine fullerene materials, and many of their derivatives, have pro-oxidant pro-oxidant properties and form adducts, which are both potentially toxic mechanisms in vivo. Although they are electrophilic with an affinity for electrons, they appear to function similarly to other non-radical electrophilic antioxidants that stimulate enzymatic oxidative stress management and protection through Nrf-2 pathways. We approach the antioxidant role of fullerene materials from the perspective that they do not act as direct free radical scavengers. Other mechanisms have been previously proposed that are more consistent with fullerene properties and antioxidant benefits in vivo. Here we discuss fullerene materials as electron scavenging antioxidants rather than free radical scavengers. We propose that fullerene materials scavenge excess electrons in the electron transport chain, preventing the formation of superoxide and hydroxyl radicals in the mitochondria. The scavenging of electrons would assist in preventing damage to mitochondrial structures and DNA, maintaining optimum oxidized to reduced nicotinamide adenine dinucleotide (NAD+/NADH) ratios, as well as in maintaining an oxidized respiratory enzyme system in mitochondrial conditions resulting from excess caloric intake in the absence of cellular energy requirements and progressive mitochondrial dysfunction related to oxidative stress. Further research is needed to evaluate the utilization of fullerene materials for use in metabolic syndromes and neurodegenerative conditions that may be complicated by progressive mitochondrial oxidative damage.

Keywords: C60; Native Aggregation; Dielectric Property; Fullerene Material; Oxidative Stress; Mitochondria; NAD+/NADH Ratio; Antioxidant; Electrophilic; Free Radical Scavenger; Electron Scavenger


  1. Wallace DC. "A mitochondrial bioenergetic etiology of disease”. Journal of Clinical Investigation 4 (2013): 1405-1412.
  2. Xiao W., et al. “NAD(H) and NADP(H) Redox Couples and Cellular Energy Metabolism”. Antioxidants & Redox Signaling 3 (2018): 251-272.
  3. Quinlan C., et al. “Sites of reactive oxygen species generation by mitochondria oxidizing different substrates”. Redox Biology 1 (2013): 304-312.
  4. Mailloux R and Harper M. “Uncoupling proteins and the control of mitochondrial reactive oxygen species production”. Free Radical Biology and Medicine 6 (2011): 1106-1115.
  5. Ziegler D., et al. “Mitochondrial effectors of cellular senescence: beyond the free radical theory of aging”. Aging Cell1 (2015): 1-7.
  6. Munro D and Treberg J. "A radical shift in perspective: mitochondria as regulators of reactive oxygen species”. The Journal of Experimental Biology 7 (2017): 1170-1180.
  7. Forman HJ., et al. “How do nutritional antioxidants really work: nucleophilic tone and para-hormesis versus free radical scavenging In vivo”. Free Radical Biology and Medicine 66 (2014): 24-35.
  8. Bourassa D. "An alternate view of fullerene material’s antioxidant activity (NOLF 4 in series: “will nanocarbon onion-like fullerenes (NOLFs) play a decisive role in the future of molecular medicine?)”. International Journal of Nanomedicine 1 (2020): 1-5.
  9. Murphy M. “How mitochondria produce reactive oxygen species”. Biochemical Journal 1 (2009): 1-13.
  10. Andrievsky GV., et al. “Peculiarities of the antioxidant and radioprotective effects of hydrated C60 fullerene nanostuctures In vitro and In vivo”. Free Radical Biology and Medicine6 (2009): 786-793.
  11. Benyamini H., et al. “Interaction of c(60)-fullerene and carboxyfullerene with proteins: docking and binding site alignment”. Bioconjugate Chemistry 2 (2006): 378-386.
  12. Orlova M., et al. “Fullerene nanoparticles operating the apoptosis and cell proliferation processes in normal and malignant cells”. Der Pharmacia Lettre 3 (2013): 99-139.
  13. Giełdoń AWM. “Rapid insight into C60 influence on biological functions of proteins”. Structural Chemistry 28 (2017): 1775-1788.
  14. Hay B and Bryantsev V. “Anion-arene adducts: C-H hydrogen bonding, anion-π interaction, and carbon bonding motifs”. Chemical Communications (2008): 2417.
  15. Di Giosia M., et al. “Proteins as supramolecular hosts for C60: a true solution of C60 in water”. Nanoscale21 (2018): 9908-9916.
  16. Shannahan J. “The biocorona: a challenge for the biomedical application of nanoparticles”. Nanotechnology Reviews 4 (2017): 345-353.
  17. Bogdanović V., et al. “Fullerenol C60(OH)24 effects on antioxidative enzymes activity in irradiated human erythroleukemia cell line”. Journal of Radiation Research.3 (2008): 321-327.
  18. Harper M., et al. “Ageing, oxidative stress, and mitochondrial uncoupling”. Acta Physiologica Scandinavica 182 (2004): 321-331.
  19. Blacker T and Duchen M. “Investigating mitochondrial redox state using NADH and NADPH autofluorescence”. Free Radical Biology and Medicine 100 (2016): 53-65.
  20. Solà M., et al. “Molecular Size and Pyramidalization: Two Keys for Understanding the Reactivity of Fullerenes”. The Journal of Physical Chemistry 99 (1995): 10752-10758.
  21. Jang J., et al. “Biological Responses of Onion-Shaped Carbon Nanoparticles”. Nanomaterials 7 (2019): 1016.
  22. Zeynalov E., et al. “Radical scavenging efficiency of different fullerenes C60-C70 and fullerene soot”. Polymer Degradation and Stability 94 (2009): 1183-1189.
  23. Cheng X., et al. “Evaluation of the structure-activity relationship of carbon nanomaterials as antioxidants”. Nanomedicine (London)7 (2018): 733-747.
  24. Stein L and Imai S. “The dynamic regulation of NAD metabolism in mitochondria”. Trends in Endocrinology and Metabolism9 (2012): 420-428.
  25. Esimbekova E., et al. “Bioluminescent assay for toxicological assessment of nanomaterials”. Doklady Biochemistry and Biophysics 1 (2017): 60-63.
  26. Bourassa D., et al. “A Retrospective Investigation of Novel Catenated Multi-shelled Fullerene-like Material for Biocompatibility after Prolonged Inhalation, Dermal, and Ingestion Exposure in Human Subjects. Part 3 in a series: Will Nanocarbon Onion-Like Fullerenes (NOLFs) Play a Decisive Role in the Future of Molecular Medicine?” EC Pharmacology and Toxicology 7 (2019): 7.
  27. Baati T., et al. “The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene”. Biomaterials26 (2012):6292-6294.
  28. DeSantis M., et al. “Abstract No. 263 - In situ nanocarbon assisted microwave therapy (NAMT) for application of targeting human prostate tumor cells in nude mice causing cytotoxic thermal ablation using extremely short cycle microwave energy as primary therapy”. Journal of Vascular and Interventional Radiology4 (2013): S116-S117.
  29. Imai S and Guarente L. “NAD+ and sirtuins in aging and disease”. Trends in Cell Biology8 (2014): 464-471.
  30. Garrido A and Djouder N. “NAD+ Deficits in Age-Related Diseases and Cancer”. Trends Cancer8 (2017): 593-610.
  31. Clement J., et al. “The Plasma NAD+ Metabolome Is Dysregulated in "Normal" Aging”. Rejuvenation Research 2 (2019): 121-130.
Citation: Daniel J Bourassa., et al. “Electron Scavenging Not Free Radical Scavenging by Fullerene Materials Protects against Mitochondrial Oxidative Stress in Complex Organisms”. EC Pharmacology and Toxicology 8.6 (2020): 61-67.

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