Research Article
Volume 8 Issue 3 - 2020
Pomegranate and Folate Ameliorate Isolation-Induced Autistic Like Behavior in Experimental Rat Model: Impact on Oxidative, Inflammatory, and Apoptotic Machineries
Azza A Ali1*, Wedad A Hassan2 and Amina K Elansary3
1Professor of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Cairo and Vice Dean of Faculty of Pharmacy, Sinai University, Kantara Branch, Egypt
2Assistant Professor of Physiology, Pharmacology Department, National Organization for Drug Control and Research (NODCAR), Giza, Egypt
3Q.C. Specialist, Pharmacology Department, National Organization for Drug Control and Research, Egypt
*Corresponding Author: Azza A Ali, Professor of Pharmacology and Toxicology, Faculty of Pharmacy, Al-Azhar University, Cairo and Vice Dean of Faculty of Pharmacy, Sinai University, Kantara Branch, Egypt.
Received: January 13, 2020; Published: February 14, 2020


Recently more infants are prone to early separation from their mothers. Meanwhile, early life experience affects neurodevelopment and stress susceptibility. Maternal separation negatively impacts development causing different psychiatric and neurodevelopmental disorders like autism. In this study, maternal separation was done through separation of pups from their dams for [1.5 hr/day - 11 days]. Seeking protection, pomegranate (500 mg/Kg) and/or folic acid (50 mg/Kg) were ingested by dams during the lactation period (20 days). Maternal separation delayed pups’ eye opening, impaired motor coordination decreased body weight and social activity in an open field arena as well as increased repetitive behavior in neonatal t-maze. Brain monoamine and reduced glutathione were decreased while nitric oxide, interleukin-1beta and caspase-3 were increased. Meanwhile intake of pomegranate and/or folic acid reversed maternal separation induced behavioral and biochemical changes. The combination protected against development of autistic-like symptoms in rats through modification of cells redox state and inflammation.

Keywords: Autism; Maternal Separation; Pomegranate; Folic Acid; Apoptosis


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Citation: Azza A Ali., et al. “Pomegranate and Folate Ameliorate Isolation-Induced Autistic Like Behavior in Experimental Rat Model: Impact on Oxidative, Inflammatory, and Apoptotic Machineries”. EC Pharmacology and Toxicology 8.3 (2020): 01-13.

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