Research Article
Volume 13 Issue 6 - 2022
Effectiveness of Purmorphamine Loaded Biodegradable 3D Polylactic Acid/Polycaprolactone/Hydroxyapatite Scaffold in a Critical-Sized Radial Bone Defect in Rat
Ahmad Oryan1*, Shadi Hassanajili2 and Sonia Sahvieh1
1Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
2Department of Chemical Engineering, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz, Iran
*Corresponding Author: Ahmad Oryan, Department of Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Received: May 09, 2022; Published: May 24, 2022




Abstract

A critical bone defect is an important problem that needs the intervention of regenerative medicine and tissue engineering to increase its healing quality at a minimum time. The polylactic acid (PLA)/polycaprolactone (PCL)/hydroxyapatite (HA) scaffold was loaded for the first time by purmorphamine (PMA), as an osteoinductive drug, and the drug delivery effect of the scaffold was tested on radius bone healing in rat. The scanning ultramicrographs, X-ray diffraction, rheology, and Fourier transform-infrared spectroscopy were used to study the scaffold features. Forty Wistar rats were divided into the following four groups (n = 10 in each group): the defect in the first group was left empty (control), and the defect of the 2nd to the 4th groups was filled by autograft, scaffold, and the PMA-loaded scaffold respectively. The level of OPN, OC, and CD31 expression was higher in the scaffold and PMA-loaded scaffold groups than the control group in the qRT-PCR (P < 0.05). The radiology, computed tomography scan, immunohistochemical, biomechanical, and histomorphometric investigations depicted a significant improvement in the PMA-loaded scaffold in comparison to the empty defect (P < 0.05). Therefore, the scaffold showed a significant influence compared to the control group (P < 0.05), and PMA promoted the potential of the PCL/PLA/HA scaffold on bone regeneration.

Keywords: PLA/PCL/HA Scaffold; Purmorphamine; Bone Regeneration; Radius Bone; Rat

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Citation: : Ahmad Oryan., et al. “Effectiveness of Purmorphamine Loaded Biodegradable 3D Polylactic Acid/Polycaprolactone/ Hydroxyapatite Scaffold in a Critical-Sized Radial Bone Defect in Rat”. EC Orthopaedics 13.6 (2022): 34-49.

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