Research Article
Volume 12 Issue 2 - 2021
Sensitivity of Antibiotic-Resistant Clinical Strains of Staphylococci and Enterococci to Biodegradation Products of Ml-10 Magnesium Alloy
Chorny Vadym Mykolayovych1, Polishchuk Natalia Mykolayivna2* and Kyryk Dmytro Leonidovych3
1Candidate of Medical Sciences, Associate Professor of the Department of Traumatology and Orthopedics, Zaporizhia State Medical University, Ukraine
2Candidate of Medical Sciences, Associate Professor of the Department of Microbiology, Virology and Immunology, Zaporizhia State Medical University, Ukraine
3Professor, Head of the Department of Microbiology, Epidemiology and Infection Control of the National Medical Academy of Postgraduate Education named after P.L. Shupyka, Kyiv, Ukraine
*Corresponding Author: Polishchuk Natalia Mykolayivna, Candidate of Medical Sciences, Associate Professor of the Department of Microbiology, Virology and Immunology, Zaporizhia State Medical University, Ukraine.
Received: December 22, 2020; Published: February 26, 2021


Introduction: The problem of development of purulent-inflammatory infections such resulting from the surgical medical care and connected with the using of metal medical alloys is urgent in medical practice. Members of genus Staphylococcus and Enterococcus are the main causative agents of these infections. These microorganisms have numerous pathogenicity factors, including the ability to form biofilms on the surface of implants. Thus, it is important the study the biological properties of magnesium-based metal alloys that have bactericidal effect and prevent to form a biofilm on the surface of the implant.

Aim of the Study: The aim of the study was to identification the antibacterial properties of medical magnesium alloy ML-10 against clinical antibiotic resistant strains of Staphylococci and Enterococci, with further opportunity to use in surgical practice as implants that contribute to the prevention of nosocomial infections.

Methods: Studies have used the ML-10 magnesium alloy extract and antibiotic-resistant clinical strains of Staphylococci and Enterococci that was isolated from the wounds of patients with infectious postoperative complications. We have incubated different concentrations of these bacteria in the extract during 120 hours and studied the antimicrobial activity of ML-10 alloy’s biodegradation products. Licensed computer software “Microsoft Excel 2010” and “Statistica for Windows 13” were used for statistical analysis.

Results: Research results have shown that antibiotic-resistant clinical strains of Staphylococci and Enterococci were sensitive to biodegradation products of ML-10 magnesium alloy. During process of the alloy biodegradation the corrosion products were formed and caused the increase pH of the medium from 7.2 to 9.3. Despite the fact that the destruction of the Staphylococcus spp. and Enterococcus spp. was slow, we found that the number of bacteria gradually decreased in the extract during the 120-hour incubation. This fact was confirmed by the decrease of the colonies number on the Mueller-Hinton agar after daily sowing from the extract. Prolonged incubation of Staphylococci and Enterococci in the extract led to complete destruction of the bacteria.

Conclusion: The biodegradation products of ML-10 magnesium alloy exhibit high antimicrobial activity against antibiotic resistant strains of Staphylococci and Enterococci. These results are making possible to use this metal in medicine as implants for prevention nosocomial infection.

Keywords: Products of Magnesium Alloy Biodegradation; Antibacterial Properties; Antibiotic Resistant Strains; Staphylococci; Enterococci


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Citation: Polishchuk Natalia Mykolayivna., et al. “Sensitivity of Antibiotic-Resistant Clinical Strains of Staphylococci and Enterococci to Biodegradation Products of Ml-10 Magnesium Alloy”.EC Orthopaedics 12.2 (2021): 41-47.

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