Research Article
Volume 11 Issue 3 - 2020
TNFA Genetic Polymorphism is Associated with Risk for Developing Hip but not Knee Osteoarthritis in Croatian Population
Randi Krog Eftedal1, Zdravko Jotanovic2, Sanja Balen3 and Zlatko Dembic1*
1Molecular Genetics Laboratory, Department of Oral Biology, University of Oslo, Norway
2University Hospital for Orthopaedics and Traumatology Lovran, School of Medicine, University of Rijeka, Croatia
3Clinical Institute for Transfusion Medicine, Universal Hospital Center Rijeka, School of Medicine, University of Rijeka, Croatia
*Corresponding Author: Zlatko Dembic, Molecular Genetics Laboratory, Department of Oral Biology, University of Oslo, Norway.
Received: January 20, 2020; Published: February 21, 2020




Abstract

Aim: We studied genetic epidemiology of primary large-joint (hip and knee) osteoarthritis (OA), in order to find disease risk factors by a candidate-gene approach. We used TNFA gene SNP rs1800629 in a case-control study in the Croatian Caucasian population. 

Method: We analyzed 225 hip and 205 knee OA patients (both with total joint replacements), and 554 healthy individuals, majority being blood donors. We genotyped for TNFA SNP rs1800629 (+308, C>T). Allelic and genotypic frequencies were compared between patients and controls.

Results: The minor allele (T) of rs1800629 significantly conferred susceptibility only to hip OA (p < 5*10-5, OR = 1.81, CI 95% = 1.34 - 2.44). The major (C) allele was significantly associated with the protection to hip (p < 5*10-5, OR = 0.63, CI 95% = 0.41 - 0.74), but not knee OA. Patients with the homozygous genotype C/C had significantly lower risk of developing hip OA (< 3*10-4, OR = 0.53, CI 95% = 0.38 - 0.76), but had no association with knee OA. The homozygosity of the minor allele (T/T) has been significantly associated with susceptibility to only hip OA (< 2*10-2, OR = 2.56, CI 95% = 1.09 - 5.57). 

Conclusion. Our findings demonstrate that major and minor alleles of the rs1800629 are associated with lower or higher risk, respectively, to developing hip, but not knee OA in the Croatian population. The data suggest a difference in the etiology of hip OA from that of the knee, perhaps due to an unknown dissimilarity in vulnerability of these joints to the actions of TNFA. Alternatively, other genetic factors including long non-protein coding LOC100287329 and/or miR6832 in vicinity of rs1800629 might be involved in the observed risk.

Keywords: Genetic Risk; TNFA; Osteoarthritis; Hip; SNP

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Citation: Randi Krog Eftedal., et al. “TNFA Genetic Polymorphism is Associated with Risk for Developing Hip but not Knee Osteoarthritis in Croatian Population”. EC Orthopaedics 11.3 (2020): 01-11.

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