Research Article
Volume 13 Issue 8 - 2018
Dose-Dependent Effect of Cyanocobalamin on the Prevention of Colon Cancer: An In Vivo Study
Smitha Padmanabhan1, Mostafa I Waly1,2*, Nejib Guizani1, Amanat Ali1, Mohammad S Rahman1, Zaher Al-Attabi1, Raya N Al-Malki1, and Varna Taranikanti3
1Department of Food Science and Nutrition, College of Agricultural and Marine Sciences, Sultan Qaboos University, Muscat, Oman
2Department of Nutrition, High Institute of Public Health, Alexandria University, Egypt
3Department of Human and Clinical Anatomy, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman
*Corresponding Author: Mostafa I Waly, Associate Professor, Food Science and Nutrition Department, Sultan Qaboos University, Oman.
Received: June 06, 2018; Published: July 30, 2018
Citation: Smitha Padmanabhan., et al .“Dose-Dependent Effect of Cyanocobalamin on the Prevention of Colon Cancer: An In Vivo Study”. EC Nutrition 13.8 (2018): 563-571.
Abstract
Azoxymethane (AOM) is an oxidizing agent that is commonly used to induce cancer in rat colon. Experimental studies strongly suggest an association between glutathione (GSH) depletion and colon cancer. GSH is an essential intracellular antioxidant, where its de novo synthesis requires cyanocobalamin, vitamin B12, yet its role on the prevention of colon cancer pathogenesis was not well studied. This study was undertaken to investigate the dose-dependent effect of cyanocobalamin supplementation in relation to GSH biosynthesis as well as its protective effect against AOM-induced oxidative stress in rat colon. Twenty-eight adult Sprague-Dawley rats were randomly divided into four groups (7 rats/group): Control group that fed standard diet with no AOM injection; and AOMtreated groups which received AOM injection and were fed one of three different diets supplemented with cyanocobalamin as follow: 25 μg, 125 μg, or 250 μg cyanocobalamin/kg of the standard diet. All rats were continuously fed with diet and watered ad libitum for 16 weeks, then they were sacrificed, and the colon tissues were examined microscopically for histological changes and homogenized for biochemical measurements of cellular oxidative stress markers (glutathione, and total antioxidant capacity). The results showed that AOM caused pathological changes in the colonic mucosal tissues and increased oxidative stress markers in rat colonic tissues. Cyanocobalamin, in a dose-dependent manner, has significantly ameliorated the AOM-mediated insults in rat colon. The results of this study provide in vivo evidence that cyanocobalamin supplementation protects against AOM-induced colonic cytotoxicity via abolishment of its colonic oxidative damage.
Keywords: Azoxymethane; Colon Cancer; Cyanocobalamin; Oxidative Stress
Copyright: © 2018 Mostafa I Waly., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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