Research Article
Volume 3 Issue 4 - 2021
Changes in Immune Phenotypes of Peripheral Blood Lymphocytes (PBLs) among Occupationally Cytostatic Exposed Hospital Employees
Anna Tompa1*, Miklós Zrínyi2 and Anna Biro3
1Semmelweis University, Institute of Public Health, Budapest, Hungary
2National Public Health Center, Toxicology Laboratory Department, Budapest, Hungary
3National Public Health Center, Toxicology Laboratory Department, Budapest, Hungary
*Corresponding Author: Anna Tompa, Semmelweis University, Institute of Public Health, Budapest, Hungary.
Received: January 27, 2021; Published: March 31, 2021




Abstract

Health professionals who had been working in different health care units and who were chronically exposed to cytostatic drugs were examined during the last 25 years. In their working area of the oncology outpatient and hospital units cytostatic drug exposure regularly exceeded safety limits due to the lack of proper safety devices. Their health condition was annually investigated by clinics, and it was found that they developed more hematological, immunological and reproductive alterations. In the present study we assessed changes in immune phenotypes based on the CD4/CD8 T-cells ratio, B and NK cell percentages measured by FACS methodology. The investigations were carried out in 550 subjects exposed to different cytostatic agents in oncology departments. Data were compared to age matched 83 healthy and non-exposed female controls. Biomarkers were measured by routine clinical laboratory tests, completed with immune phenotyping and measured in peripheral blood lymphocytes (PBL). Health personnel exposed to different agents showed increased ratio of CD4/CD8 T-cell and a decrease in activated T-cells. Beside these parameters we found an elevation of B-cells and a decrease in NK-cells. Within the exposed groups smokers showed even stronger decreased NK-cell counts compared to non-smokers. These changes in immune-phenotypes may give us a new biomarker immune suppression of PBL-cells among nurses after toxic exposures in their working environment.

Keywords: Occupational Exposure of Cytostatic Drugs; Immune Depression; CD4/CD8 Ratio; NK-Cells; B-Cells

References

    1. Aw D and Silva AB. “Immune senescence emerging challenges for an ageing population”. Immunology 120 (2007): 435-446.
    2. Biro A., et al. “Immunotoxicity Monitoring of Hospital Staff Occupationally Exposed to Cytostatic Drugs”. Pathology Oncology Research 17 (2011): 301-308.
    3. Biro A and Tompa A. “Life Style Factors Influencing Immunotoxic Effects Detected on Peripheral Blood Lymphocytes of Health Personnel Exposed to Anesthetic Gas (2013)”. Clinical and Experimental Pharmacology S4 (2013): 2161-1459.
    4. Carrano AV and Natarajan AT. “Considerations on population monitoring using cytogenetic techniques. ICPEMC Publ.No.14”. Mutation Research 204 (1988): 379-406.
    5. Condore G., et al. “Biology of longevity: role of the innate immune system”. Rejuvenation Research 1 (2006): 143-148.
    6. Epel ES., et al. “Accelerated telomere shortening in response to life stress”. Proceedings of the National Academy of Sciences of the United States of America 49 (2004): 17312-1715.
    7. Hakin FT and Gress RE. “Immune senescence: deficit in adaptive immunity in elderly”. Tissue Antigens3 (2007): 179-189.
    8. Hayflick L and Moorhead P. “The serial cultivation of human diploid cell strains”. Experimental Cell Research3 (1961): 585-621.
    9. Kiecolt-Glaser JK., et al. “Chronic stress and age – related increases in the proinflammatory cytokine IL-6”. Proceedings of the National Academy of Sciences of the United States of America 15 (2003): 9090-9095.
    10. Koch S., et al. “Multiparameter flow cytometric analysis of CD4 and CD8-T cell subsets in young and old people”. Immunity and Ageing 5 (2008): 6.
    11. Lewis DE., et al. “Tumor necrosis factor alpha and CD80 modulate CD28 expression through a similar mechanism of T-cell receptor-independent inhibition of transcription”. Journal of Biological Chemistry 28 (2004): 29130-29138.
    12. Phelouzat MA., et al. “Excessive apoptosis of mature T lymphocytes is a characteristic feature of human immune senescence”. Science Direct1-2 (1996): 25-38.
    13. Pole A., et al. “Oxidative stress, cellular senescence and ageing”. AIMS Molecular Science 3 (2016): 300-324.
    14. Tannenbaum TN and Goldberg RJ. “Exposure to anesthetic gases and reproductive outcome: a review of the epidemiologic literature”. Journal of Occupational and Environmental Medicine 27 (1985): 659-668.
    15. Tompa A., et al. “Chemical Safety and Health Conditions among Hungarian Hospital Nurses”. Annals New York Academy of Acience 1076 (2006): 635-648.
    16. Tompa A. “Hospital workplaces: risks of exposure to carcinogens”. Hospital Pharmacy Europe 36 (2008): 29-31.
    17. Tompa A., et al. “Genotoxic Monitoring of Nurses Handling Cytotoxic Drugs”. Asia-Pacific Journal of Oncology Nursing4 (2016): 365-369.
    18. Van de Veen W., et al. “Role of regulatory B cells in immune tolerance to allergens and beyond”. The Journal of Allergy and Clinical Immunology 3 (2016): 654-665.
    19. Klinker MW and Lundy SK. “Multiple mechanisms of immune suppression by B lymphocytes”. Molecular Medicine 18 (2012): 123-137.
    20. Elshaer NS. “Adverse health effects among nurses and clinical pharmacists handling antineoplastic drugs: Adherence to exposure control methods” (2017).
    21. Gajski G., et al. “Genotoxicity assessment of selected cytostatic drug mixture in human lymphocytes: A study based on concentration relevant for occupational exposure”. Environmental Research 161 (2018): 26-34.
Citation: Anna Tompa., et al. “Changes in Immune Phenotypes of Peripheral Blood Lymphocytes (PBLs) among Occupationally Cytostatic Exposed Hospital Employees”. EC Nursing and Healthcare 3.4 (2021): 245-250.

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