Research Article
Volume 12 Issue 2 - 2020
New Pathogenetic Approaches to Inhibit the Growth of Glioblastoma Relapses
N Ya Gridina1*, AN Morozov1, VD Rozumenko1, Yu V Ushenin2, AA Shmeleva1, NG Draguntsova1 and OI Veselova1
1Institute of Neurosurgery n. A. P. Romodanova, Kiev, Ukraine
2Institute of Semiconductor Physics n. V. E. Lashkaryova, Kiev, Ukraine
*Corresponding Author: N Ya Gridina, Institute of Neurosurgery n. A. P. Romodanova, Kiev, Ukraine.
Received: November 27, 2019; Published: January 11, 2020




Abstract

Currently, many researchers support the view, that if tumor microenvironment is normalized by suppressing tumor-associated inflammation (TAI), tumor stem cells, which only 3 - 5% contains in the tissues of primary glioblastomas, can lose their malignant properties. In the work we showed the possibility of TAI II stage normalizing by blocking the activity of NMDA - dependent calcium channels with low concentrations of verapamil - hydrochloride, that, as a result, led to a significant increase in the life expectancy of patients with glioblastomas. The patients were investigated in the distant postoperative period and treated with such concentrations of verapamil - hydrochloride, that minimize the level of blood cells aggregation in II stage of the inflammatory process. 12 patients received treatment (I group), of these 9 patients died, and 3 patients continued treatment with verapamil. 37 patients did not received treatment (II group) with calcium blocker using verapamil - hydrochloride examples in pills. For the objectification of TAI, that present’s in the patient’s body, the definition of blood cells aggregation was examined on the “Plasmon” sensor. Peripheral blood cells were collected from patients to determine the indicators of blood cells aggregation with the addition of various aqueous dilutions of verapamil - hydrochloride (from 1:10 to 1:100,000 times). Patients took drugs at the lowest level of blood cells aggregation doses. Drug dose was performed by decreasing its concentration by a factor of 10, 000. From the 49 patients, admitted to the clinic, only 3 patients did not undergo chemotherapy at will. After the surgical removal of glioblastoma and the postoperative irradiation course, they only took verapamil - hydrochloride at low concentration daily without interruption for life). All 3 patients continue to live for 20, 26 and 29 months, respectively. In the I group of 9 patients, undergoing combined treatment courses and taking verapamil - hydrochloride, the average life expectancy was 20.4 ± 6.8 months and 9.11 ± 1.5 months in the group of 37 patients (Cox’s F - test p = 0,00063). The paper presents for the first time the results of highly malignant glioblastoma treatment with low concentrations of the NMDA-dependent calcium blocker verapamil - hydrochloride. The results indicate a high antitumor activity of the drug, both when used together with traditional methods (9 patients) of treatment or separately (3 patients).

Keywords: Glioblastoma Relapses; Tumor-Associated Inflammation; Blood Cells Aggregation, Surface Plasmon Resonance, Low Concentrations of Verapamil

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Citation: N Ya Gridina., et al. “New Pathogenetic Approaches to Inhibit the Growth of Glioblastoma Relapses”. EC Neurology 12.2 (2020): 01-09.

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