Research Article
Volume 12 Issue 10 - 2020
NO and H2O2 Modulate Ca2+, Ca2+ Dependent K+ Currents and Intracellular Calcium Concentration of Cultured, Identified Lymnaea Neurons
Hadi Fathi-Moghadam1 and William Winlow2,3*
1Physology Research Centre, Department of Physiology, School of Medicine, Ahwaz Jundishapur University of Medical Sciences, Ahwaz, Iran
2Dipartimento di Biologia, Università Degli Studi di Napoli Federico II, Napoli, Italy
3Institute of Ageing and Chronic Diseases, The APEX building, University of Liverpool, UK
*Corresponding Author: William Winlow, Dipartimento di Biologia, Università Degli Studi di Napoli Federico II, Napoli, Italy and Institute of Ageing and Chronic Diseases, The APEX building, University of Liverpool, UK.
Received: September 01, 2020; Published: September 30, 2020




Abstract

The actions of NO and H2O2 on gross K+ and Ca2+ currents were investigated on cultured identified neurons of the mollusc Lymnaea stagnalis (L).

Pedal I Cluster (RPeI) neurons were isolated and cultured for 1 - 5 days and K+ and Ca2+ currents were measured in the whole cell patch clamp configuration.

Both H2O2 (3 µM to 300 µM) and NO (0.1 mM to 1 mM) substantially decreased L-type Ca2+ and K+ currents in a concentration-dependent manner. These effects were partially reversible.

Both free radicals also increased intracellular calcium concentration ([Ca2+]i) in these neurons, clarifying an earlier study.

Keywords: Nitric Oxide; Hydrogen Peroxide; Ca2+ Dependent K+ Current; Ca2+ Current; [Ca2+]i, Identified Neurons; Cell Culture; Lymnaea stagnalis

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Citation: Hadi Fathi-Moghadam and William Winlow. “NO and H2O2 Modulate Ca2+, Ca2+ Dependent K+ Currents and Intracellular Calcium Concentration of Cultured, Identified Lymnaea Neurons”. EC Neurology 12.10 (2020): 38-49.

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