Research Article
Volume 12 Issue 10 - 2020
Modeling of Post Traumatic Glial Scar after Controlled Cryodestruction of Rat Spinal Cord
Georgy Telegin1, Alexey Minakov1, Aleksandr Chernov1, Vasiliy Manskikh2, Stanislav Kaprovoy2, Anton Alekhin3 and Aldo Spallone4,5*
1Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Prospect Nauki, Pushchino, Russian Federation
2M. V. Lomonosov Moscow State University, Leninskie Gory, Moscow, Russian Federation
3Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, Moscow, Russian Federation
4Department of Nervous Diseases, RUDN Russian People’s Friendship University, Ulitsa Miklukho-Maklaya, Moscow, Russian Federation
5NCL-Neuromed, Institute of Neurosciences, Via Patrica, Rome, Italy
*Corresponding Author: Aldo Spallone, Professor, Department of Nervous Diseases, RUDN Russian People’s Friendship University, Ulitsa Miklukho-Maklaya, Moscow, Russian Federation.
Received: July 29, 2020; Published: September 30, 2020




Abstract

Spinal cord injury (SCI) represents a relevant problem under both clinical e social points of view. Post traumatic glial scarring is recognized as one of the main factors which would affect negatively axons regrowth following SCI. As in the situation of SCI occurring in humans, presently available animal experimental model of SCI provoke a wide range of glial scarring reaction as a result of trauma, thus investigating the specific issue of post traumatic glial scarring would require an extremely high number of animal due to significant inter animals variability. We recently introduced a new model of experimental SCI in rats where a controlled unilateral cryo-induced lesion was created. Sprague-Dawley rats of adequate size were used, microsurgical unilateral exposure of lower thoracic spinal cord dura was performed, and cryolesion was induced via a controlled exposure to -20° temperature for one minute using liquid azote. The animals were sacrificed following 30 days of close observation during which they developed a consistent unilateral lower limb deficit. Microscopical examination of the injury site demonstrated spinal cord tissue vacuolization of rather consistent sites and surrounded by glial scarring very similar in extension in different animals. Preliminary results indicate that either produced lesions either glial scarring are rather consistent. This model may be of value in investigating the possible therapeutic role of pharmacological protocols for SCI.

Keywords: Spinal Cord; Experimental Model; Cryoinjury; Glial Scar; Controlled Spinal Lesion

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Citation: Georgy Telegin., et al. “Modeling of Post Traumatic Glial Scar after Controlled Cryodestruction of Rat Spinal Cord”. EC Neurology 12.10 (2020): 117-121.

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