Research Article
Volume 12 Issue 10 - 2020
Modeling of Post Traumatic Glial Scar after Controlled Cryodestruction of Rat Spinal Cord
Georgy Telegin1, Alexey Minakov1, Aleksandr Chernov1, Vasiliy Manskikh2, Stanislav Kaprovoy2, Anton Alekhin3 and Aldo Spallone4,5*
1Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Prospect Nauki, Pushchino, Russian Federation
2M. V. Lomonosov Moscow State University, Leninskie Gory, Moscow, Russian Federation
3Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, GSP-7, Ulitsa Miklukho-Maklaya, Moscow, Russian Federation
4Department of Nervous Diseases, RUDN Russian People’s Friendship University, Ulitsa Miklukho-Maklaya, Moscow, Russian Federation
5NCL-Neuromed, Institute of Neurosciences, Via Patrica, Rome, Italy
*Corresponding Author: Aldo Spallone, Professor, Department of Nervous Diseases, RUDN Russian People’s Friendship University, Ulitsa Miklukho-Maklaya, Moscow, Russian Federation.
Received: July 29, 2020; Published: September 30, 2020


Spinal cord injury (SCI) represents a relevant problem under both clinical e social points of view. Post traumatic glial scarring is recognized as one of the main factors which would affect negatively axons regrowth following SCI. As in the situation of SCI occurring in humans, presently available animal experimental model of SCI provoke a wide range of glial scarring reaction as a result of trauma, thus investigating the specific issue of post traumatic glial scarring would require an extremely high number of animal due to significant inter animals variability. We recently introduced a new model of experimental SCI in rats where a controlled unilateral cryo-induced lesion was created. Sprague-Dawley rats of adequate size were used, microsurgical unilateral exposure of lower thoracic spinal cord dura was performed, and cryolesion was induced via a controlled exposure to -20° temperature for one minute using liquid azote. The animals were sacrificed following 30 days of close observation during which they developed a consistent unilateral lower limb deficit. Microscopical examination of the injury site demonstrated spinal cord tissue vacuolization of rather consistent sites and surrounded by glial scarring very similar in extension in different animals. Preliminary results indicate that either produced lesions either glial scarring are rather consistent. This model may be of value in investigating the possible therapeutic role of pharmacological protocols for SCI.

Keywords: Spinal Cord; Experimental Model; Cryoinjury; Glial Scar; Controlled Spinal Lesion


  1. Nas K., et al. “Rehabilitation of spinal cord injuries”. World Journal of Orthopedics 1 (2015): 8-16.
  2. Wang H., et al. “Portrait of glial scar in neurological diseases”. International Journal of Immunopathology and Pharmacology 31 (2018): 2058738418801406.
  3. King VR., et al. “The use of injectable forms of fibrin and fibronectin to support axonal ingrowth after spinal cord injury”. Biomaterials 31 (2010): 4447-4456.
  4. Wei Y., et al. “Hyaluronic acid hydrogel modified with Nogo-66 receptor antibody and poly-L-lysine to promote axon regrowth after spinal cord injury”. Journal of Biomedical Materials Research Part B: Applied Biomaterials 95 (2010): 110-117.
  5. Minakov AN., et al. “Experimental models of spinal cord injury in laboratory rats”. Acta Naturae3 (2018): 4-10.
  6. Alizadeh A., et al. “Traumatic Spinal Cord Injury: an overview of Pathophysiology, models and acute injury mechanisms”. Frontiers in Neurology 10 (2019): 282.
  7. Minakov A., et al. “Surgical model of spinal cord injury in rats”. Abstract of 14th Felasa Congress 2019. Laboratory Animals 1 (2019): 130.
  8. Vasiliev SA., et al. “Brain cryodestruction in mammals In vivo”. Khirurgiia (Monsk) 10 (2010): 62-68.
  9. Vasiliev SA., et al. “Cryodestruction of brain tumors: cryodestruction technique and technology”. Clinical Experiment Sur Petrovsky Journal 1 (2015): 15-21.
  10. Ilfeld BM., et al. “Ultrasound-guided percutaneous cryoneurolysis for treatment of acute pain: coned cryoanalgesia replace continuous peripheral nerve blocks?” British Journal of Anaesthesia 4 (2017): 703-706.
  11. De Leo JA., et al. “Characterization of a neuropathia pain model: sciatic cryoneurolysis in the rat”. Pain1 (1994): 9-16.
  12. Kerns JM., et al. “A comparison of cryoprobe and crush lesions in the rat sciatic nerve”. Pain1 (1991): 31-39.
  13. Burnett MG and Zager EL. “Pathophysiology of peripheral nerve injury: a brief review”. Neurosurgical Focus 5 (2004): 1-7.
  14. Feng Y Yan T., et al. “Wld (S), Nmnats and exam degeneration-progress in the past two decades”. Protein Cell3 (2010): 237-245.
  15. Hsu M and Stevenson FF. “Reduction unmuscular mobility by selective focused cold therapy: a preclinical study”. Journal of Neural Transmission1 (2014): 15-20.
  16. Gaudet AD., et al. “Wallerian degeneration: gaining perspective on inflammatory events after peripheral nerve injury”. Journal of Neuroinflammation 8 (2011): 110.
  17. Scheib J and Höke A. “Advances in peripheral nerve regeneration”. Nature Reviews Neurology 12 (2013): 668-676.
  18. Suganuma S., et al. “Uncultured adipose-derived regenerative cells promote peripheral nerve regeneration”. The Journal of Orthopaedic Science 1 (2013): 145-151.
  19. Gordon T. “Neurotrophic factor expression in denervated motor and sensory Schwann cells: relevance to specificity of peripheral nerve regeneration”. Experimental Neurology 254 (2014): 99-108.
  20. Aikeremujiang M., et al. “Femoral nerve regeneration and its accuracy under different injury mechanisms”. Neural Regeneration Research 10 (2015): 1669-1673.
  21. Zhou L., et al. “Mechanism research of cryoanalgesia”. Journal of Neurology Research 4 (1995): 307-311.
  22. Guo SZ., et al. “Preparation of the acellular scaffold of the spinal cord and the study at biocompatibility”. Spinal Cord 48 (2010): 576-581.
  23. Barnard D., et al. “Cryoanalgesia in the management of chronic facial pain”. Journal of Oral and Maxillofacial Surgery 2 (1981): 101-102.
  24. Zakrzewska JM., et al. “Cryotherapy in the management of paroxysmal trigeminal neuralgia. Four year follow up of 39 patients”. Journal of Oral and Maxillofacial Surgery 14 (1986): 5-7.
  25. Gage AA. “History of cryosurgery”. Seminars in Surgical Oncology 2 (1998): 99-109.
  26. Collins GH., et al. “The histopathology of freezing injury to the rat spinal cord. A light and electron microscope study. II. Repair and regeneration”. Journal of Neuropathology and Experimental Neurology 6 (1986): 742-757.
  27. Ilfeld BM., et al. “Novel cryoneurolysis device for the treatment of sensory and motor peripheral nerves”. Expert Review of Medical Devices 8 (2016): 713-725.
Citation: Georgy Telegin., et al. “Modeling of Post Traumatic Glial Scar after Controlled Cryodestruction of Rat Spinal Cord”. EC Neurology 12.10 (2020): 117-121.

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