Research Article
Volume 12 Issue 7 - 2020
Mobilization of Intracellular Calcium by Ryanodine in Cultured, Identified Molluscan Neurons is Cell Specific
IA Ahmed1, PM Hopkins2,3 and W Winlow4,5*
1Faculty of Medicine, University of Garden City, Khartoum, Sudan
2Leeds Institute of Medical Research at St James’s, University of Leeds, Leeds, United Kingdom
3Malignant Hyperthermia Investigation Unit, St. James’s University Hospital, Leeds, United Kingdom
4Department of Biology, University of Naples Federico II, Via Cintia, Naples, Italy
5Institute of Ageing and Chronic Diseases, University of Liverpool, Liverpool, United Kingdom
*Corresponding Author: W Winlow, Department of Biology, University of Naples Federico II, Via Cintia, Naples, Italy and Institute of Ageing and Chronic Diseases, University of Liverpool, Liverpool, United Kingdom.
Received: June 11, 2020; Published: June 30, 2020




Abstract

  • Previously we showed that the calcium mobilizing agent, ryanodine, altered action potential shape in some identified neurons of the mollusc Lymnaea stagnalis.
  • Here we investigate the actions of ryanodine (1 µM and 5 µM) on intracellular calcium concentration [Ca2+]i in the same identified neurons in single cell culture.
  • The free [Ca2+]i levels in these neurons were determined using the cell permeable Ca2+ indicator Fura-2AM, both in the presence and absence of extracellular calcium (0-Ca2+/EGTA).
  • In the presence of extracellular calcium, both concentrations of ryanodine produced a substantial increase in [Ca2+]i in three neuron types (VV1or 2, RPD1 and RPD2), whereas, in the fourth neuron type (RPeD1) there was a decrease below resting level.
  • The free [Ca2+]i remained elevated even after washing with normal saline, except in one case (VV1or 2) where it was found to be reversible. However, there was a general decline in the level of [Ca2+]i after addition of ryanodine in the absence of Ca2+ in the external medium.
  • Therefore, extracellular Ca2+ is a prerequisite for any appreciable rise in [Ca2+]i due to ryanodine.

  Keywords: Ryanodine; Intracellular Calcium Concentration; Lymnaea Neurons; Neuron Specificity

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Citation: W Winlow., et al. “Mobilization of Intracellular Calcium by Ryanodine in Cultured, Identified Molluscan Neurons is Cell Specific”. EC Neurology 12.7 (2020): 67-75.

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