Research Article
Volume 12 Issue 7 - 2020
Malted Barley Ameliorated Spinal Cord Injuries of Offspring of Hypercholesterolemic Mother Rats
Zahraa A Greash1, Osama A Abbas1 and Hassan I El-Sayyad2*
1Zoology Department, Faculty of Science, Port Said University, Egypt
2Zoology Department, Faculty of Science, Mansoura University, Egypt
*Corresponding Author: Hassan I El-Sayyad, Zoology Department, Faculty of Science, Mansoura University, Egypt.
Received: May 08, 2020; Published: June 29, 2020


The prevalence of obesity and neurodegenerative diseases is high due to the consumption of diet rich in cholesterol. However, there was no data available about the injuries of spinal cord of breast feeding young. The current research work has demonstrated the ameliorative effect of malted barley feeding on the injuries of spinal cord of offspring maternally ingested on a high cholesterol diet.

Sixty virgin female and twenty adult male albino rats (Rattus norvegicus) weighing 100 gm body weight. A diet consisting of 3 percent cholesterol was consumed by virgin females for 4 months before conception and during gestation and lactation period. Virgin rats were made pregnant by mating with healthy males and onset of gestation was determined by observing sperm within the vaginal smears in the next morning. The pregnant were divided into 4 groups (n = 15); control (C), hypercholesterolemic (H) (3%) with or without malted barley (20%) feeding (H+B) group. Offsprings of the studied groups were sacrificed at 21 days-old. Their cervical spinal cord was removed and investigated histologically, immunohistochemically of glial fibrillary acidic protein (GFAP) and proliferating cell nuclear antigen (PCNA) and transmission electron microscopy. Also, biochemical assessments of glutathione-s-transferase and superoxide dismutase, malondialdehyde, caspase-3, 8-hdG, neurotransmitters (DA, 5-HT and GABA) and cytokines (IL6& 10) were carried out. The present results showed that ingestion of malted barley to mothers fed on a high cholesterol diet restored the ependymal canal epithelium, vesicular structure of neuronal cells and myelination of the nerve axons. They also, observed increased immunohistochemistry of PCNA and decreased immunostaining of GFAP. The assayed antioxidant enzymes and neurotransmitters contents of the spinal cord were increased. However, the apoptic markers caspase-3, 8-hdG and cytokines expressing inflammation were decreased.

The author concluded that barley supplementation in combination with high cholesterol diet ameliorated the spinal injuries due to its high contents of antioxidants which scavenges free radicals.

Keywords: Spinal Cord; Histology; Transmission Electron Microscopy; Antioxidants; Neurotransmitters


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Citation: Hassan I El-Sayyad., et al. “Malted Barley Ameliorated Spinal Cord Injuries of Offspring of Hypercholesterolemic Mother Rats”. EC Neurology 12.7 (2020): 92-104.

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