Research Article
Volume 12 Issue 5 - 2020
LPS-Induced Inflammation Alters Cytokines in Amniotic Fluid and Serum of Pregnant Wistar Rats and their Offspring
Ana Claudia Januario1*, Alexandre Ademar Hoeller1, Joana Margarida Gaspar2,3, Roberta de Paula Martins2,4, Tuany Eichwald2, Monique Michels5, Felipe Dal-Pizzol5, João Carlos Xikota1,6,7 and Maria Marlene de Souza Pires1,6,7
11Programa de Pós Graduação em Ciências Médicas, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil
22Laboratório de Bioenergética e Estresse Oxidativo (LABOX), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil
33Programa de Pós Graduação em Bioquímica, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil
44Departamento de Ciências da Saúde, Universidade Federal de Santa Catarina (UFSC), Araranguá, SC, Brazil
45Laboratório de Fisiopatologia Experimental, Universidade do Extremo Sul Catarinense (UNESC), Criciúma, Brazil
46Laboratório de Nutrologia e Metabologia (MENULAB), Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil
47Departamento de Pediatria, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil
*Corresponding Author: Ana Claudia Januario, Programa de Pós Graduação em Ciências Médicas, Universidade Federal de Santa Catarina (UFSC), Florianópolis, SC, Brazil.
Received: April 03, 2020; Published: April 20, 2020


Intrauterine inflammation is a major cause of early pregnancy loss and impaired fetal growth, besides associated with the biogenesis of neuropsychiatric disorders such as autism spectrum and schizophrenia. Animal models of maternal immunity activation aim to elucidate the inflammatory mechanisms and their relationship with neurodevelopmental disorders. Thus, we investigated the effects of LPS-induced inflammation at different doses in female rats and their relation with the duration of the inflammatory event and maternal changes. Pregnant Wistar rats were grouped and treated once (on E17) or 3 times (on E15-E17) with LPS (15 or 150 μg/kg, IP). LPS at a concentration of 150 μg/kg, both in single and repeated doses, impaired gestational weight gain, in addition to cause death in 23.07% of the rats and significantly decreasing the number of live-born pups. LPS injection increased the level of TNF-α in the amniotic fluid which decreased 72 hours after injection, with no changes in these cytokines in the maternal serum. IL1-β was increased in amniotic fluid after a single dose of LPS (150 μg/kg) but decreased after the application of 3 doses, with a difference between the duration of treatments at this dose. Also, was increased in the offspring serum following 3 repeated doses of LPS 150 μg/kg. IL-6 was increased in amniotic fluid at a single dose of 150 μg/kg and decreased after three days of daily LPS injections. There was an increase in maternal serum IL-6 levels at doses of 15 μg/kg applied once and 150 μg/kg after 3 consecutive injections. IL-10 was increased in amniotic fluid after both doses of LPS applied once but decreased 72 h after application of the highest dose. In the maternal serum, only the highest dose increased IL-10 after 72 hours of LPS. These findings demonstrate that even a single dose of low LPS concentration is sufficient to activate the maternal immune system and may have a direct implication on gestational progress that could affect fetal development.

Keywords: Gestation; Inflammation; LPS; Cytokines; Autism


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Citation: Ana Claudia Januario., et al. “LPS-Induced Inflammation Alters Cytokines in Amniotic Fluid and Serum of Pregnant Wistar Rats and their Offspring”. EC Neurology 12.5 (2020): 42-50.

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