Research Article
Volume 12 Issue 4 - 2020
Some Aspects of the Systemic Mechanism of Brain Malignant Gliomas Progression and Methodological Approaches to its Correction
N Ya Gridina1*, AN Morozov1, VD Rozumenko1, YuV Ushenin2, LP Shvachko3, VN Klyuchka1 and AD Beloysova1
1Institute of Neurosurgery, A.P. Romodanova, Kiev, Ukraine
2Institute of Semiconductor Physics, V. E. Lashkaryova, Kiev, Ukraine
3Institude of Molecular Biology and Genetic, Kiev, Ukraine
*Corresponding Author:N Ya Gridina, Institute of Neurosurgery, A. P. Romodanova, Kiev, Ukraine.
Received: February 17, 2020; Published: March 27, 2020




Abstract

The growth of malignant gliomas was considered at a systemic level, which allows us to understand the basic shifts in the protective-compensatory reactions of the body, leading to the progression of brain tumors. It was shown that activation of NMDA-receptors affects the transmembrane potential, the indices of which are mediated through the level of aggregation of blood cells of patients with malignant gliomas. An increase in the level of aggregation of blood cells corresponds to stage II of the in vivo inflammatory process, which is a protective-compensatory reaction of the body. Verapamil is an NMDA- receptor dependent blocker of slow calcium L- channels, because they localized in the structure of NMDA- receptors. Using a highly sensitive Plasmon biosensor, the level of aggregation of blood cells was studied before surgery and on the 7th day after surgery. The effect of low concentrations of verapamil-hydrochloride on the indicators of blood cell aggregation in in vitro experiments made it possible to determine the differences between non-tumor inflammation and tumor-associated inflammation in glioblastomas. Tumor-associated inflammation is aseptic microinflammation, which is rarely detected by traditional laboratory tests and requires the use of biosensor equipment. Further, the use of verapamil-hydrochloride in dilutions with distillate water 10,000 times allowed to reveal the mechanism of “non-healing wounds” in malignant tumors. The oxidation enzymes of the polyamines DAO and PAO, which carry out repair processes in the III stage of inflammation, at low concentrations of verapamil- hydrochloride were characterized by a sharp decrease in activity in glioblastomas compared with identical indices in spinal hernias. These results are one of the experimental confirmations of the incompleteness of stage III of inflammation in malignant gliomas of the brain. This leads to the transition of reparative processes during inflammation to the processes of regeneration by mesenchymal stem cells through the epithelial - mesenchymal transition (EMT) and the subsequent progression of the tumor. It was shown that verapamil - hydrochloride in low concentrations reduces the expression of Snail, one of the main EMT genes, by 50%. The existing relationship between the development of glioma progression and the activity of ionotropic receptors that affect the transmembrane potential, defines a new methodology for therapeutic measures in the postoperative period. It consists in the suppression of tumor-associated inflammation by inhibitors of ionotropic receptors and ion channel blockers, stabilization of cell membranes, and inhibition of EMT. These therapeutic effects should be carried out after completing courses of any types of traditional therapy for malignant tumors.

Keywords: Glioblastoma; Systemic Mechanism of Progression; Tumor-Associated Inflammation; Blood Cells Aggregation; Surface Plasmon Resonance; Low Concentrations of Verapamil

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Citation: N Ya Gridina., et al. “Some Aspects of the Systemic Mechanism of Brain Malignant Gliomas Progression and Methodological Approaches to its Correction”. EC Neurology 12.4 (2020): 80-90.

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