Research Article
Volume 16 Issue 3 - 2020
Serum Interferon-γ and Interleukin-12 in Relation to IFNγ, IL12A and IL12B Gene Polymorphisms in BCG Osteitis Survivors
Matti Korppi1*, Johanna Teräsjärvi2, Eero Lauhkonen1, Heini Huhtala3 and Qiushui He2,4
1Center for Child Health Research, Faculty of Medicine and Biotechnology, University of Tampere and University Hospital, Tampere, Finland
2Institute of Biomedicine, University of Turku, Turku, Finland
3Faculty of Social Sciences, University of Tampere, Tampere, Finland
4Department of Medical Microbiology, Capital Medical University, Beijing, China
*Corresponding Author: Matti Korppi, Professor, Center for Child Health Research, Faculty of Medicine and Biotechnology, University of Tampere and University Hospital, Tampere, Finland.
Received: November 21, 2019; Published: February 27, 2020


Background: Interferon-γ (IFN-γ) and interleukin-12 (IL-12) are involved in responses to mycobacteria including BCG strains in the BCG vaccine. The aim of the study was to evaluate IFN-γ and IL-12 concentrations in serum in relation to two IFNγ, two IL12A and one IL12B polymorphisms, and to compare serum IFN-γ and IL-12 concentrations with ex vivo IFN-γ or IL-12 production in BCG-stimulated cell cultures.

Methods: Serum samples were obtained from 132 adults with BCG osteitis in infancy and good-quality samples were available for IFN-γ (N = 128) and IL-12p70 (N = 130) measurements using the Bio‐Plex Pro human IL 10 immunoassay kit with the Bio‐Plex 200 System. Serum IFN-γ and IL-12p70 concentrations were compared in relation to the IFNγ rs2430561, IFNγ rs35314021, IL12A rs568408, IL12A rs2243115 and IL12B rs3212227 genotypes, as well as in relation to ex vivo IFN-γ and IL-12p70 concentrations in BCG-stimulated cell cultures.

Results: There was no significant association between the five included IFNγ, IL12A or IL12B genotypes and serum IFN-γ or IL-12p70 concentrations. The result remained negative, when serum IFN-γ and IL-12p70 concentrations were compared between ten ex vivo low producers and those 104 who were not, respectively.

Discussion: The IFNγ rs2430561, IFNγ rs35314021, IL12A rs568408, IL12A rs2243115 and IL12B rs3212227 polymorphisms had no impact on IFN-γ and IL-12 production. Since IFN-γ and IL-12 are no doubt involved in responses to mycobacteria BCG included, other IFNγ and IL12 polymorphisms need to be included in future studies.

Keywords: Bacillus Calmette-Guerin; BCG osteitis; Gene Polymorphism; Interferon-γ; Interleukin-12; IFNγ Gene; IL12A Gene; IL12B Gene


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  4. Abebe F. “Is interferon-gamma the right marker for bacille Calmette-Guérin-induced immune protection? The missing link in our understanding of tuberculosis immunology”. Clinical Experimental Immunology 169.3 (2012): 213-219.
  5. Korppi M., et al. “Interferon-γ and interleukin-12 production in relation to gene polymorphisms in BCG osteitis”. Pediatrics International 61.10 (2019): 982-987.
  6. Auton A., et al. “The 1000 Genomes Project Consortium. A global reference for human genetic variation”. Nature 526.7571 (2015): 68-74.
  7. Korppi M., et al. “Toll-like receptor 1, 2 and 6 polymorphisms: no association with 11 serum cytokine concentrations”. Acta Paediatrica 107.12 (2018): 2217-2218.
  8. Korppi M., et al. “TOLLIP rs5743854 and rs1169387 gene polymorphisms in Bacillus Calmette-Guerin osteitis”. EC Microbiology 15.5 (2019): 377-381.
  9. Lin WL., et al. “Management of Bacillus Calmette-Guerin osteomyelitis/osteitis in immunocompetent children - A systematic review”. Vaccine 33.8 (2015): 4391-4397.
Citation: Matti Korppi., et al. “Serum Interferon-γ and Interleukin-12 in Relation to IFNγ, IL12A and IL12B Gene Polymorphisms in BCG Osteitis Survivors”. EC Microbiology 16.3 (2020): 01-05.

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