Research Article
Volume 18 Issue 7 - 2022
Test for the Cholinesterase Activity in Diatoms Ulnaria ulna Cells
VV Roshchina*
Institute of Cell Biophysics, Federal Research Centre, Pushchino Scientific Centre for Biological Research, Russian Academy of Sciences, Pushchino, Moscow, Russia
*Corresponding Author: VV Roshchina, Institute of Cell Biophysics, Federal Research Centre, Pushchino Scientific Centre for Biological Research, Russian Academy of Sciences, Pushchino, Moscow, Russia.
Received: May 26, 2022; Published: June 23, 2022




Abstract

Testing of cholinesterase, the enzyme that hydrolyzed acetylcholine, in diatoms Ulnaria ulna has been carried out by the histochemical methods with Ellman reagent, Fast Red TR, and azo analogue of Ellman reagent dithio-bis-(p-phenyleneazo)-bis-(1-oxy-8-chloro-3,6) sodium disulfate. Last dye test was better observed visually and under microscope. Inhibitors of cholinesterase neostigmine, and physostigmine decreased the hydrolysis of acetylthiocholine by the cells.

 

Keywords: Acetylthiocholine; Ellman Reagent; Fast Red TR Salt; Red Analogue of Ellman Reagent; Neostigmine; Physostigmine

References

  1. Fluck RA and Jaffe MJ. “Cholinesterases from plant tissues. VI. Distribution and subcellular localization in Phaseolus aureus Roxb”. Plant Physiology 53 (1974): 752-758.
  2. Maheshwari SC., et al. “Cholinesterases in plants”. In: Recent Developments in Plant Sciences: S.M Sircar Memorial Volume. Edition. Sen S.P. New Delhi: Today and Tomorrows printers and publishers. New Delhi (1982): 145-160.
  3. Yamamoto K and Momonoki YS. “Plant Acetylcholinesterase plays an important role in the response to environmental stimuli”. In: Neurotransmitters in Plants: Perspectives and Applications. Eds. A. Ramakrishna and V.V. Roshchina. CRC Press, Boca Raton (2018): 9-33.
  4. Yamamoto K., et al. “Altered expression of acetylcholinesterase gene in rice results in enhancement or suppression of shoot gravitropism”. Plant Signaling and Behavior4 (2016).
  5. Gupta A and Gupta R. “A survey of plants for presence of cholinesterase activity”. Phytochemistry 46 (1997): 827-831.
  6. Podunai Yu A., et al. “Mating System And Two Types Of Gametogenesis In The Fresh Water Diatom Ulnaria ulna (Bacillariophyta)”. Algology 1 (2014): 3-19.
  7. Davidovich NA., et al. “Reproductive properties of diatoms significant for their cultivation and biotechnology”. Russian Journal of Plant Physiology 62/2 (2015): 153-160.
  8. Gorun V., et al. “Modified Ellman procedure for assay of cholinesterase in crude enzymatic preparations”. Analytical Biochemistry 86 (1978): 324-326.
  9. Kakariari E., et al. “Purification and characterization of an intracellular esterase from Propionibacterium freudenreichii ssp. freudenreichii ITG 14”. Le Lait 5 (2000): 491-501.
  10. Roshchina VV. “Tools for microanalysis of the neurotransmitter location in plant cell. In: Neurotransmitters in Plants: Perspectives and Applications”. Editions. A. Ramakrishna and V.V. Roshchina. CRC Press, Boca Raton (2018): 135-146.
  11. Budantsev AYu and Roshchina VV. “Enzymatic Tissue Biotests (MAO and AChE biotests) and Bioindicators”. In: Macro, Micro, and Nano-Biosensors. Potential Applications and Possible Limitations. Editors: Rai, M., Reshetilov, A., Plekhanova, Y., Ingle, A.P. (Editions.), Springer. Springer International Publishing AG (Cham, Switzerland) (2020): 37-55.
  12. Roshchina VV. “Neurotransmitters in plant life”. Enfield, Plymouth: Science Publ 283 (2001).
  13. Roshchina VV. “New tendency and perspectives in evolutionary considerations of neurotransmitters in microbial, plant, and animal cells”. In: Advances in Experimental Medicine and Biology, Edition. Lyte. M. Ed. New York, Heidelberg: Springer. V 874 (2016): 25-77.
Citation: VV Roshchina. “Test for the Cholinesterase Activity in Diatoms Ulnaria ulna Cells”. EC Microbiology 18.7 (2022): 34-38.

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