Research Article
Volume 18 Issue 7 - 2022
Test for the Cholinesterase Activity in Diatoms Ulnaria ulna Cells
VV Roshchina*
Institute of Cell Biophysics, Federal Research Centre, Pushchino Scientific Centre for Biological Research, Russian Academy of Sciences, Pushchino, Moscow, Russia
*Corresponding Author: VV Roshchina, Institute of Cell Biophysics, Federal Research Centre, Pushchino Scientific Centre for Biological Research, Russian Academy of Sciences, Pushchino, Moscow, Russia.
Received: May 26, 2022; Published: June 23, 2022


Testing of cholinesterase, the enzyme that hydrolyzed acetylcholine, in diatoms Ulnaria ulna has been carried out by the histochemical methods with Ellman reagent, Fast Red TR, and azo analogue of Ellman reagent dithio-bis-(p-phenyleneazo)-bis-(1-oxy-8-chloro-3,6) sodium disulfate. Last dye test was better observed visually and under microscope. Inhibitors of cholinesterase neostigmine, and physostigmine decreased the hydrolysis of acetylthiocholine by the cells.


Keywords: Acetylthiocholine; Ellman Reagent; Fast Red TR Salt; Red Analogue of Ellman Reagent; Neostigmine; Physostigmine


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Citation: VV Roshchina. “Test for the Cholinesterase Activity in Diatoms Ulnaria ulna Cells”. EC Microbiology 18.7 (2022): 34-38.

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