Research Article
Volume 18 Issue 6 - 2022
Galactooligosaccharides Production by Transgalactosylation of Lactose using Yeast β-Galactosidases Isolated from Milk Kefir Grains
Camargo Vânia Eliza1, Ishimoto Caroline Kie1, Araújo Ticiana Vasques1*, Nurmberguer Jussimara Monteiro2, Ayub Marco Antonio Zachia3 and Esposito Elisa1
1Departamento de Ciência e Tecnologia, Universidade Federal de São Paulo, São José dos Campos Brazil
2Associação Fundo de Incentivo a Pesquisa, AFIP, Departamento de Pesquisa e Desenvolvimento de Medicina Diagnostica, São Paulo, Brazil
3Instituto de Ciência e Tecnologia dos Alimentos, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil
*Corresponding Author: Araújo Ticiana Vasques, Departamento de Ciência e Tecnologia, Universidade Federal de São Paulo, São José dos Campos Brazil.
Received: May 14, 2021; Published: May 24, 2022




Abstract

Kefir grains from two different origins were cultivated in different media containing lactose as a carbon source. The isolates were grown in liquid medium with 20% lactose to access their ability to produce β-galactosidase, which was measured by permeabilizing cells and measuring the enzymatic activity. The microorganisms showing the highest enzymatic activities were submitted to a high lactose concentration solution in order to induce the transgalactosylation reaction and form galactooligosaccharides (GOS) molecules. A total of twenty-six isolates were obtained, of which twenty produced β-galactosidase activity and seven strains were further used for GOS production. The isolate SP - J, which showed the highest enzymatic activity, was identified by the MALDI-TOF/ MS technique as being the yeast Kluyveromyces marxianus (99.99 % confidence). Incubations of this yeast in 200 mg/mL of lactose solutions at pH 6.5 and 40 °C produced the highest concentration of GOS (39.34 mg/ml) using a small amount of β-galactosidase (0.51 U/mL). Kefir grains proved to be a source of efficient microorganisms, in addition to low cost to produce β-galactosidase by lactose transgalactosylation of the prebiotic GOS.

Keywords: Galactooligosaccharides; Β-Galactosidase; Kefir Grains; Kluyveromyces Marxianus; Lactose; Transgalactosylation

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Citation: Araújo Ticiana Vasques., et al. “Galactooligosaccharides Production by Transgalactosylation of Lactose using Yeast β-Galactosidases Isolated from Milk Kefir Grains”. EC Microbiology 18.6 (2022): 10-22.

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