Research Article
Volume 18 Issue 5 - 2022
In vitro Assessment of Antimicrobial Activity of Honey Bees and Nigella sativa Against Selected Clinical Isolates from Shendi City

Ghanem M Mahjaf1, Leila M Abdelgadeir1, Aisha Y Ibrahim2, Nedal A Mohammed2, Sulafa A Mohammed2, Salih O Mohammed3, Mosab Nouraldein Mohammed Hamad4 * and Naser E Bilal2

1Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Shendi University, Sudan

2Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Khartoum University, Sudan

3Medicinal and Aromatic Plants Research Institute and Traditional Medicine, National Center for Research, Khartoum, Sudan

4Department of Parasitology and Medical Entomology, College of Health and Allied Sciences, St. Joseph University, Dar Es Salaam, Tanzania

*Corresponding Author: Mosab Nouraldein Mohammed Hamad, 4Department of Parasitology and Medical Entomology, College of Health and Allied Sciences, St. Joseph University, Dar Es Salaam, Tanzania.
Received: March 16, 2022; Published: April 29, 2022


Background: An alarming rise in bacterial strains resistant to existing antimicrobial agents requires a renewed effort to seek agents effective against pathogenic bacteria resistant to antimicrobials. The new antimicrobial agents are will overcome this problem.

Objective: This study aimed to study the in vitro antimicrobial activity of different concentrations of Honey, petroleum ether extracts of medicinal plant Nigella sativa (seeds), and the mixture using the cup-plate agar diffusion method.

Materials and Methods: This cross-sectional observational study was carried out among One hundred samples were collected from urine, wound, ear, and eye swab, 95/100 (95%) showed bacterial growth, from which six types of pathogenic bacteria at Shendi city hospitals during March to June 2018.

Results: Of the total 95 clinical and standard specimens were confirmed as Staphylococcus aureus 20 (21%), Escherichia coli 20 (21%), Pseudomonas aeruginosa 15 (16%), and Klebsiella pneumoneae 30 (31%). In addition to Candida albicans 10 (11%). These results showed the activity of antimicrobial Petroleum ether extract of the Nigella sativa, and honey pronounced dose-dependent on standard strains and clinical isolates, while methanolic extract of Nigella sativa explained no activity.

Conclusion: The activity of Nigella sativa, honey, and the mixture exhibited high antimicrobial activity against all types of tested organisms both clinical and standard organisms. Therefore Nigella sativa, honey, and the mixture can be regarded as a broad-spectrum antimicrobial agent.


Keywords: Antimicrobial Activity; Honey Bees; Nigella sativa; Petroleum Ether Extracts; Methanolic Extract


  1. WHO (World health organization)., Fact sheet no. 134, Traditional medicine (2016).
  2. Cassir Rolain JM and Drouqui P. “A new strategy to fight antimicrobial resistance: the revival of old antibiotics”. Frontiers in Microbiology 5 (2014): 551-555.
  3. Gupta C., et al. “Antimicrobial activity of some herbal oils against common food-borne pathogens”. African Journal of Microbiology Research 2 (2008): 258-261.
  4. Shelef LA. “Antimicrobial effects of spices”. Journal of Food Safety1 (1984): 29-44.
  5. Zohary D and Hopf M. “Domestication of plants in the old world: the origin and spread of cultivated plants in West Asia”. Europe, and the Nile Valley Oxford University Press, New York. 3rd edition (2000): 206.
  6. Hannan A., et al. “Antibacterial activity of Nigella sativa against clinical isolates of methicillin resistant staphylococcus aureus”. Journal of Ayub Medical College Abbottabad3 (2008): 7274.
  7. Alandejani T., et al. “Effectiveness of honey on Staphylococcus aureus and Pseudomonas aeruginosa biofilms”. Otolaryngology-Head and Neck Surgery1 (2009): 114-118.
  8. Alqurashi AM., et al. “Antibacterial activity of Saudi honey against Gram negative bacteria”. Journal of Microbiology and Antimicrobials1 (2013): 1-5.
  9. Srinivasan D., et al. “Antimicrobial activity of certain Indian medicinal plants used in folkoric medicine”. Journal of Ethnopharmacology 74 (2001): 217-220.
  10. Ibrahim TA., et al. “Antibacterial activity of herbal extracts against multidrug resistant of bacteria from clinical origin”. Journal of life Science Leaf Lets 15 (2011): 490-498.
  11. Kakil ES. “Antibacterial Activity of Zingiber officinal, Matricaria chamomilla and Nigella sativa Extracts on Growth of pathogenic Bacteria Isolated from Different Clinical specimens”. Sc. Thesis, Sudan University of Science and Technology (2013).
  12. Toama MA., et al. “Antimicrobial activity of the volatile oil of Nigella sativa Linneaus seeds”. Antimicrobial Agents and Chemotherapy2 (1974): 225-226.
  13. Islam SK., et al. “Antifungal activities of the oils of Nigella sativa seeds”. Pakistan Journal of Pharmaceutical Sciences1 (1989): 25-28.
  14. Bilal NE., et al. “Antimicrobial effect of N. sativa on selected Microorganism”. The Journal of Hepatology Gastroenterology Infect Diseases JHGID4 (1996): 105-111.
  15. Ara N., et al. “In vitro antimicrobial activity of the volatile oil of Nigella Sativa linn seeds”. TAJ: Journal of Teachers Association2 (2005): 109-112.
  16. Zuridah H., et al. “In vitro antibacterial activity of Nigella sativa against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli and Bacillus cereus”. Asian Journal of Plant Sciences3 (2008): 331-333.
  17. Salman MT., et al. “Antimicrobial activity of seed oil and extract against multidrug resistant clinical strains of Pseudomonas aeruginosa”. Hippocatic Journal of Unani Medicine4 (2009): 95-104.
  18. Hyungjaer Lee., et al. “The antimicrobial activity of honey from different floral sources on bacterial isolates”. International Journal of Food Microbiology1-2 (2007): 249-244.
  19. Nur Azid., et al. “The bacterial activity of different honey against pathogenic bacteria”. Archives of Medical Research5 (2010): 464-467.
Citation: Mosab Nouraldein Mohammed Hamad., et al.In vitro Assessment of Antimicrobial Activity of Honey Bees and Nigella sativa Against Selected Clinical Isolates from Shendi City”. EC Microbiology 18.5 (2022): 72-81.

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