Research Article
Volume 18 Issue 5 - 2022
In vitro Assessment of Antimicrobial Activity of Honey Bees and Nigella sativa Against Selected Clinical Isolates from Shendi City

Ghanem M Mahjaf1, Leila M Abdelgadeir1, Aisha Y Ibrahim2, Nedal A Mohammed2, Sulafa A Mohammed2, Salih O Mohammed3, Mosab Nouraldein Mohammed Hamad4 * and Naser E Bilal2

1Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Shendi University, Sudan

2Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, Khartoum University, Sudan

3Medicinal and Aromatic Plants Research Institute and Traditional Medicine, National Center for Research, Khartoum, Sudan

4Department of Parasitology and Medical Entomology, College of Health and Allied Sciences, St. Joseph University, Dar Es Salaam, Tanzania

*Corresponding Author: Mosab Nouraldein Mohammed Hamad, 4Department of Parasitology and Medical Entomology, College of Health and Allied Sciences, St. Joseph University, Dar Es Salaam, Tanzania.
Received: March 16, 2022; Published: April 29, 2022




Abstract

Background: An alarming rise in bacterial strains resistant to existing antimicrobial agents requires a renewed effort to seek agents effective against pathogenic bacteria resistant to antimicrobials. The new antimicrobial agents are will overcome this problem.

Objective: This study aimed to study the in vitro antimicrobial activity of different concentrations of Honey, petroleum ether extracts of medicinal plant Nigella sativa (seeds), and the mixture using the cup-plate agar diffusion method.

Materials and Methods: This cross-sectional observational study was carried out among One hundred samples were collected from urine, wound, ear, and eye swab, 95/100 (95%) showed bacterial growth, from which six types of pathogenic bacteria at Shendi city hospitals during March to June 2018.

Results: Of the total 95 clinical and standard specimens were confirmed as Staphylococcus aureus 20 (21%), Escherichia coli 20 (21%), Pseudomonas aeruginosa 15 (16%), and Klebsiella pneumoneae 30 (31%). In addition to Candida albicans 10 (11%). These results showed the activity of antimicrobial Petroleum ether extract of the Nigella sativa, and honey pronounced dose-dependent on standard strains and clinical isolates, while methanolic extract of Nigella sativa explained no activity.

Conclusion: The activity of Nigella sativa, honey, and the mixture exhibited high antimicrobial activity against all types of tested organisms both clinical and standard organisms. Therefore Nigella sativa, honey, and the mixture can be regarded as a broad-spectrum antimicrobial agent.

 

Keywords: Antimicrobial Activity; Honey Bees; Nigella sativa; Petroleum Ether Extracts; Methanolic Extract

References

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Citation: Mosab Nouraldein Mohammed Hamad., et al.In vitro Assessment of Antimicrobial Activity of Honey Bees and Nigella sativa Against Selected Clinical Isolates from Shendi City”. EC Microbiology 18.5 (2022): 72-81.

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