Research Article
Volume 18 Issue 5 - 2022
Expression of TLR2 and TLR4 Receptors on Lymphoid Cells as a Marker of Infectious Lesions of Periodontal Tissues, Associated with the Dominance of Porphyromonas gingivalis and Filifactor alocis in Periodontal Biofilm

Tsarev Victor Nikolaevich1*, EN Nikolaeva1, IP Balmasova1,2, EV Ippolitov1, MS Podporin1 and EM Gretsov3

1Scientific Research Medical and Dental Institute, Moscow State University of Medicine and Dentistry, Moscow, Russia

2Department of Allergology and Immunology, Russian University of Friendship of Peoples of the World, Moscow, Russia

3Hematological Research Center of the Ministry of Public Health of Russia, Moscow, Russia

*Corresponding Author: Tsarev Victor Nikolaevich, Scientific Research Medical and Dental Institute, Moscow State University of Medicine and Dentistry, Moscow, Russia.
Received: September 08, 2021; Published: April 21, 2022




Abstract

A research was carried out of the Toll-like receptors TLR2 and TLR4 expression on non-stimulated peripheral blood cells (neutrophils, monocytes and lymphocytes) and gingival fluid cells in people with healthy periodontal disease and in patients with chronic periodontitis associated with osteopenia and osteoporosis. P. gingivalis and F. alocis, asaccharolytic anaerobic bacteria, are emerging markers of periodontitis. It was established that the expression of TLR2 and TLR4 on peripheral blood leukocytes in different people, despite of their study group, varied significantly. Multidirectional expression of TLR2 and TLR4 on peripheral blood cells was found (both upward and downward) in patients with chronic periodontitis associated with P. gingivalis, F. alocis.

 

Keywords: TLR2; TLR4; Receptors; P. gingivalis; F. alocis; Periodontitis; Osteoporosis; Osteopenia

References

  1. Flemmig TF and Beikler T. “Control of oral biofilms”. Periodontology 20001 (2011): 9-15.
  2. Murakami T. “Understanding and treatment strategy of the pathogenesis of periodontal disease based on chronic inflammation”. Clinical Calcium's5 (2016): 766-772.
  3. Bayrakova AL., et al. “The role and biological significance of Toll-like receptors in immunity”. Vestnik RAMN 1 (2008): 45-54.
  4. Papadopoulos G., et al. “Immunologic environment influences macrophage response to Porphyromonas gingivalis”. Molecular Oral Microbiology3 (2017): 250-261.
  5. Wilensky A., et al. “The role of natural killer cells in periodontitis”. Periodontology 20001 (2015): 128-141.
  6. Kwon Y., et al. “Regulation of Bone Cell Differentiation and Activation by Microbe-Associated Molecular Patterns. Review”. International Journal of Molecular Sciences11 (2021): 5805.
  7. Krauss JL., et al. “Complementary Tolls in the periodontium: How periodontal bacteria modify complement and Toll-like receptor responses to prevail in the host”. Periodontology 2000 52 (2010): 141-162.
  8. Moutsopoulos NM and Konkel JE. “Tissue specific immunity at the oral mucosal barrier”. Trends in Immunology4 (2018): 276-287.
  9. Trejo-de la OA., et al. “Relevance of single-nucleotide polymorphisms in human TLR genes to infectious and inflammatory diseases and cancer”. Genes and Immunity4 (2014): 199-209.
  10. Mukherjee S., et al. “Toll-like receptor polymorphism in host immune response to infectious diseases: A review”. The Scandinavian Journal of Immunology1 (2019): e12771.
  11. Koren O., et al. “Human oral, gut, and plaque microbiota in patients with atherosclerosis”. Proceedings of the National Academy of Sciences of the United States of America1 (2011): 4592-4598.
  12. Nichols FC., et al. “Unique lipids from a common human bacterium represent a new class of Tool-like receptor 2 ligands capable of enhancing autoimmunity”. The American Journal of Pathology6 (2009): 2430-2438.
  13. Kim HY., et al. “Characterization and immunostimulatory activity of extracellular vesicles from Filifactor alocis”. Molecular Oral Microbiology1 (2020): 1-9.
  14. Song MK., et al. “Filifactor alocis - derived extracellular vesicles inhibit osteogenesis through TLR2 signaling”. Molecular Oral Microbiology5 (2020): 202-210.
  15. Khaitov RM., et al. “Manual of clinical immunology diagnosis of diseases of the immune system”. Moscow: GEOTAR-Media (2009): 345.
  16. Burns E., et al. “Cutting Edge: TLR2 is required for the innate response to Porphyromonas gingivalis: Activation leads to bacterial persistence and TLR2 deficiency attenuates induced alveolar bone resorption”. Journal of Immunology 177 (2006): 8296-8300.
  17. Wang YH., et al. “Porphyromonas gingivalis lipids inhibit osteoblastic differentiation and function”. Infection and Immunity9 (2010): 3726-3735.
  18. Zhang YKJ., et al. “TLR-2 – depended modulation of osteoclastogenesis Porphyromonas gingivalis through differential induction of NFAtc1 and NF-(kappa)B”. Journal of Biological Chemistry 27 (2011): 24159-24169.
  19. Li Y., et al. “MicroRNA-15b shuttled by bone marrow mesenchymal stem cell-derived extracellular vesicles binds to WWP1 and promotes osteogenic differentiation”. Arthritis Research and Therapy 1 (2020): 269.
  20. Jia L., et al. “Pathogenesis of Important Virulence Factors of Porphyromonas gingivalis via Toll-Like Receptors”. Frontiers in Cellular and Infection Microbiology 9 (2019): 262.
  21. Olsen I and Nichols FC. “Are sphingolipids and serine dipeptide lipids underestimated virulence factors of Porphyromonas gingivalis”. Infection and Immunity Journal 7 (2018): e00035-18.
  22. Nichols FC., et al. “Phosphorilated dihydroceramides from common human bacteria are recovered in human tissues”. PLoS One 2 (2011): e16771.
  23. Zhou Q., et al. “The use of TLR2 modified BMSCs for enhanced bone regeneration in the inflammatory micro-environment”. Artificial Cells, Nanomedicine, and Biotechnology 1 (2019): 3329-3337.
  24. Miralda I., et al. “Whole Transcriptome Analysis Reveals That Filifactor alocis Modulates TNFα-Stimulated MAPK Activation in Human Neutrophils”. Frontiers in Immunology 11 (2020): 497.
  25. Mathews JB., et al. “Hyperactivity and reactivity of peripheral blood neutrophils in chronic periodontitis”. Clinical and Experimental Immunology2 (2007): 255-264.
Citation: Tsarev Victor Nikolaevich., et al. “Expression of TLR2 and TLR4 Receptors on Lymphoid Cells as a Marker of Infectious Lesions of Periodontal Tissues, Associated with the Dominance of Porphyromonas gingivalis and Filifactor alocis in Periodontal Biofilm”. EC Microbiology 18.5 (2022): 33-42.

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