Volume 17 Issue 9 - 2021
Rapid Antigen Testing for Detection of SARS-CoV-2 (COVID-19) Infection
Attapon Cheepsattayakorn1,2*, Ruangrong Cheepsattayakorn3 and Porntep Siriwanarangsun1
1Faculty of Medicine, Western University, Pathumtani Province, Thailand
210th Zonal Tuberculosis and Chest Disease Center, Chiang Mai, Thailand
3Department of Pathology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand
*Corresponding Author: Attapon Cheepsattayakorn, 10th Zonal Tuberculosis and Chest Disease Center, Chiang Mai, Thailand.
Received: August 07, 2021; Published: August 30, 2021

N and S proteins of SARS-CoV-2 (COVID-19) are the main immunogens among the four structural proteins (E, M, N, and S) [1]. The N protein-IgG ELISA provides a sensitivity of 94.7% that is significant higher than that of the S protein-IgG ELISA [2]. Antibodies against N proteins are longer-lived and have greater volume, in comparison to E, M, and S proteins [2]. Approximately, 100 companies are manufacturing or developing rapid antigen tests (RATs), one of the four types (virus isolation with cell cultures, serological testing, RATs, and molecular techniques) [2] for SARS-CoV-2 (COVID-19) detection [3]. Most RATs for SARS-CoV-2 (COVID-19) detection use a simple-to-use lateral flow test format that commonly use for influenza, malaria, and HIV testing as a sandwich immunodetection [4]. The testing results are interpreted by the operator within 10 to 30 minutes after collecting the respiratory specimen and applying it to the test strip [5]. In comparison to the nucleic acid amplification tests (NAATs), a decreasing sensitivity is found in the trade-off for simplicity of RATs for SARS-CoV-2 (COVID-19) operation [4]. As of September 11, 2020, only three companies submitted documents towards WHO’s Emergency Use Listing (EUL) procedure, two tests have been approved by Japan’s Pharmaceutical and Medical Devices Agency, and only four tests have received United States Food and Drug Administration (FDA) Emergency Use Authorization (EUA) [6,7]. In comparison to NAATs in respiratory specimens (nasal or nasopharyngeal swabs), the specificity is consistently high (> 97%), whereas the sensitivity ranges from 0 to 94% [8-17


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Citation: Attapon Cheepsattayakorn., et al. “Rapid Antigen Testing for Detection of SARS-CoV-2 (COVID-19) Infection”. EC Microbiology 17.9 (2021): 18-20.

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