Research Article
Volume 17 Issue 3 - 2021
The In-Silico Study on Structural, Functional and Sub-Cellular Localization of Hypothetical Proteins in the Orf Virus
Hitesh S Thakare1*, Dilip Meshram2 and Arun B Ingle1
1Department of Microbiology, Seth Kesarimal Porwal College, Kamptee, Maharashtra, India
22Department of Zoology, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur, Maharashtra, India
*Corresponding Author: Hitesh S Thakare, Department of Microbiology, Seth Kesarimal Porwal College, Kamptee, Maharashtra, India.
Received: April 13, 2020; Published: February 27, 2021




Abstract

The function, sub-cellular localization and structure of hypothetical proteins in the Orf Virus were explained by this study. The Orf Virus (OFRV) having a total of 130 NCBI genes out of which 42 are predicted for hypothetical proteins and these hypotheticals proteins were unknown for function, sub-cellular localization and structure and which was screened for function, sub-cellular localization and structure. For the determination of functional annotations the bioinformatics online tools as CDD-BLAST, INTERPROSCAN and PFAM were used. Cello v2.5 was used for the prediction of the sub-cellular localization and PS2 Server-Protein Structure Prediction server was used for the prediction of templates for conserved domains. To determine the 3-D structures, E-value and aligned percentage of the hypothetical proteins the PS2 Server-Protein Structure Prediction server also used. The study on this Orf virus may be useful for understanding the functional and structural characteristics of hypothetical protein as well as understanding the genetics at the molecular level to control Orf virus infection through vaccination.

Keywords: ORFV; Contagious Ecthyma; E-Value; Parapoxvirus; Pfam

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Citation: Hitesh S Thakare., et al. “The In-Silico Study on Structural, Functional and Sub-Cellular Localization of Hypothetical Proteins in the Orf Virus". EC Microbiology 17.3 (2021): 10-20.

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