Research Article
Volume 17 Issue 2 - 2021
Adaptation of Whole Cell Kinetic Model Template, UniKin1, to Escherichia coli Whole Cell Kinetic Model, ecoJC20
Jeremy Leejun Cho1,2 and Maurice HT Ling1,2,3,4*
1Department of Applied Sciences, Northumbria University, United Kingdom
2School of Life Sciences, Management Development Institute of Singapore, Singapore
3HOHY PTE LTD, Singapore
4School of Data Sciences, Perdana University, Malaysia
*Corresponding Author: Maurice HT Ling, School of Life Sciences, Management Development Institute of Singapore, Singapore.
Received: December 14, 2020; Published: January 30, 2021




Abstract

Mathematical modelling can be used to study metabolism involving thousands of biochemical reactions and kinetic models (KMs) of metabolism enable the time-course analysis of metabolic changes. Recently, a universal whole cell KM of central metabolism, UniKin1, has been presented. Here, we adapt UniKin1, into an Escherichia coli specific model by modifying the initial concentrations for 48.7% (n = 149) of the metabolites and 25.2% (n = 78) of the enzymes into E. coli specific concentrations, and term our model as ecoJC20. Our simulation results suggest that ecoJC20 is substantially different from UniKin1. We also demonstrate the potential of ecoJC20 to evaluate the effects of transgenic nitrogen fixation pathways on the central metabolism; thus, underpinning the potential applications of kinetic models as an experimental design tool.

 

Keywords: Whole Cell Kinetic Model; Simulation; Escherichia coli; Computer-Aided Design (CAD)

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Citation: Jeremy Leejun Cho and Maurice HT Ling. “Adaptation of Whole Cell Kinetic Model Template, UniKin1, to Escherichia coli Whole Cell Kinetic Model, ecoJC20”. EC Microbiology 17.2 (2021): 254-260.

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