Research Article
Volume 17 Issue 3 - 2021
HDAC6 Inhibition Aggravates the Lyme Disease-Associated Inflammation
Meerambika Mishra, Lindsey Powers, Crystal L Vanhorn and Bikash Sahay*
Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA
*Corresponding Author: Bikash Sahay, Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida, Gainesville, FL, USA.
Received: July 13, 2020; Published: February 27, 2021




Abstract

Histones are basic proteins that wrap around DNA for conditional accessibility of DNA to their transcription; however, HDAC6 is a unique histone deacetylase capable of deacetylating both histone and non-histone proteins. Recently HDAC6 has been shown to play an inflammatory role by limiting p38-MAPK activation in an LPS sepsis model. Here, we tested the ability of HDAC6 inhibition to control inflammation initiated by B. burgdorferi both in vitro and in vivo. The data indicate inhibition of HDAC6 leads to an increase in pro-inflammatory cytokines in macrophages and mice. HDAC6 deficient macrophages revealed similar results when co-incubated with B. burgdorferi. Inhibition of HDAC6 also resulted in diverse responses when exposed to different TLR ligands, suggesting a more complex role of HDAC6 in the inflammatory process. Additionally, inhibition of HDAC6 led to an increased acetylation of tubulin; however, there was minimal impact on p38-MAPK phosphorylation.

Keywords: HDAC6; Tubastatin A; Borrelia; Inflammation; TLR

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Citation: Bikash Sahay., et al. “HDAC6 Inhibition Aggravates the Lyme Disease-Associated Inflammation”. EC Microbiology 17.3 (2021): 77-86.

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