Research Article
Volume 16 Issue 10 - 2020
Bioelectricity Generation Using Marine Sediment and Cow Dung
BO Uba1*, KN Obidike1, CU Dokubo2 and ID Nnaodi1
1Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria
2Department of Science Laboratory Technology, Delta State Polytechnic Ogwashi - Uku, Delta State, Nigeria
*Corresponding Author: BO Uba, Lecturer, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.
Received: June 18, 2020; Published: September 10, 2020


In this study, the aim is to determine the application of marine waste and sediment mixed with cow dung as the substrate for generation of electricity by means of microbial fuel cell technology. The waste water sample and sediments were collected from Abonema water site at Port Harcourt Rivers State. The method employed for isolation, characterization and identification of electrogenic microbes involves the isolation on Nutrient agar, microscopy, biochemical tests (Analytical Profile Index (API) kit) and comparison with Bergey’ Manual of Determinative Bacteriology. The electrogenic organisms isolated include: Pseudomonas aeruginosa, Corynebacterium sp.,and Lactobacillus casei. Three (3) MFCs were fabricated with 1L transparent plastic for the cathode and anode chambers containing graphite electrode and PVC pipe salt bridge. The experiment was performed at room temperature with continuous monitoring and readings were taken after 24h of inoculation. The method employed for the multimetric measurement and physicochemical parameters involves voltage, current, power density, temperature, pH, conductivity; total dissolved solid (TDS) and total organic carbon (TOC). The method involved for biofilm formation and development involve coverslip overlay microscopic techniques. The result showed that MFC setup 1 generated the highest voltage and current of 391 mV and 126 mA. The pH and temperature of the MFC setups revealed slight acidicity to neutrality as well as moderate temperatures. There was biofilm development from 24h to 120h. Statistically, there is significant difference (P < 0.05) between the test sample and the control in all the parameters measured. This study revealed that the microbial fuel cell fabricated with the substrates above has a higher voltage and current productivity than the positive and negative control.

Keywords: Bacteria; Bioelectricity; Marine Ecosystem; Microbial Fuel Cell


  1. Pant D., et al. “A review of the substrates used in microbial fuel cells (MFCs) for sustainable energy production”. Bioresource Technology6 (2010): 1533-1543.
  2. Moon SJ., et al. “Microbial Fuel cell a promising technology for electricity production from a variety of materials”. Current Biology18 (2006): 1812-1817.
  3. Chang IS., et al. “Improvement of a microbial fuel cell performance as a BOD sensor using respiratory inhibitors”. Journal on Biosensbioelectron 20 (2005): 1856-1859.
  4. Kim IS., et al. “Evaluation of procedures to acclimate a microbial fuel cell for electricity production”. Applied Microbiology and Biotechnology 68 (2005): 23-30.
  5. Liu H., et al. “Production of electricity from acetate or butyrate using a single-chamber microbial fuel cell”. Environmental Science and Technology 39 (2005): 658-662.
  6. Ragauskas AJ., et al. “The path forward for biofuels and biomaterials”. Journal on Sciences 311 (2006): 484-489.
  7. Song C. “Fuel processing for low-temperature and high-temperature fuel cells: Challenges opportunities for sustainable development in the 21st century”. Catalogue Today 77 (2008): 17-49.
  8. He Z., et al. “Electricity generation from artificial waste water using an upflow microbial fuel cell”. Environmental Science and Technology 39 (2005): 5262-5267.
  9. You S., et al. “A microbial fuel cell using permanganate as a cathodic electron acceptor”. Journal on Power Sources 162 (2006): 1409-1415.
  10. Gil GC., et al. “Operational parameters affecting the performance of a mediator-less microbial fuel cell”. Biosens Bioelectron 18 (2003): 327-334.
  11. Kim JR., et al. “Sustainable wastewater treatment: how might microbial fuel cells contribute”. Biotechnology Advances 286 (2010): 871-881.
  12. Du Z., et al. “A state of the art review on microbial fuel cells: A promising technology for waste water treatment and bioenergy”. Biotechnology Advance 25 (2007): 464-482.
  13. Zhao F., et al. “Application of pyrolyzed iron (II) phthalocyanine and CoTMPP based oxygen reduction catalysts as cathode materials in microbial fuel cells”. Biotechnology Letters 30 (2006): 1771-1776.
  14. Denmark D. “Cow dung a source of green energy”. Journal of Sciences 25 (2015): 11-13.
  15. Adeleye SA and Okorondu SI. “Bioelectricity from students’ hostel waste water using microbial fuel cell”. International Journal of Biological and Chemical Sciences2 (2015): 1038-1049.
  16. Singh D., et al. “Microbial fuel cell: A green technology for power generation”. Annals of Biological Research3 (2010): 128-138.
  17. Association of Official Analytical Chemists (AOAC). “Official method of analysis”. Association of Official Analytical Chemists, Washington DC, USA. 19th edition (2012): 121-130.
  18. Nelson DW and Sommers LE. “A rapid and accurate method for estimating organic carbon in soil”. Proceedings of Seminar on the Petroleum Industry and the Nigerian Environment. NNPC/FMW and H, Warri, Nigeria (1975): 164.
  19. Logroño W., et al. “Bioelectricity generation from vegetables and fruits wastes by using single chamber microbial fuel cells with high Andean soils”. Energy Procedia 75 (2015): 2009-2014.
  20. Willey JM., et al. “Prescott, Harley and Kleins Microbiology”. McGraw-Hill companies, New York, 7th edition (2008): 537-813.
  21. Cheesbrough M. “District laboratory practice in tropical countries”. Cambridge University Press, New York USA. Part 2, 2nd edition (2006): 38-70.
  22. Dasgupta D., et al. “Biofilm-mediated enhanced crude oil degradation by newly isolated Pseudomonas species”. ISRN Biotechnology 250749 (2013): 1-13.
  23. Jannelli N., et al. “Low pH, high salinity: Too much for microbial fuel cells”? Applied Energy 192 (2016): 543-550.
  24. Nastro RA., et al. “Microbial fuel cells in solid waste valorization: trends and applications”. Singapore: Springer (2017): 159-171.
  25. Wang Z., et al. “Spontaneous thallium (I) oxidation with electricity generation in singlechamber microbial fuel cells”. Applied Energy 209 (2018): 33-42.
  26. Wang H., et al. “Substrate removal and electricity generation in a membrane-less microbial fuel cell for biological treatment of wastewater”. Bioresource Technology 138 (2013): 109-116.
  27. Zhi W., et al. “Methods for understanding microbial community structures and functions in microbial fuel cells: A review”. Bioresource Technology 171 (2014): 461-468.
Citation: BO Uba., et al. “Bioelectricity Generation Using Marine Sediment and Cow Dung”. EC Microbiology 16.10 (2020): 01-12.

PubMed Indexed Article

EC Pharmacology and Toxicology
LC-UV-MS and MS/MS Characterize Glutathione Reactivity with Different Isomers (2,2' and 2,4' vs. 4,4') of Methylene Diphenyl-Diisocyanate.

PMID: 31143884 [PubMed]

PMCID: PMC6536005

EC Pharmacology and Toxicology
Alzheimer's Pathogenesis, Metal-Mediated Redox Stress, and Potential Nanotheranostics.

PMID: 31565701 [PubMed]

PMCID: PMC6764777

EC Neurology
Differences in Rate of Cognitive Decline and Caregiver Burden between Alzheimer's Disease and Vascular Dementia: a Retrospective Study.

PMID: 27747317 [PubMed]

PMCID: PMC5065347

EC Pharmacology and Toxicology
Will Blockchain Technology Transform Healthcare and Biomedical Sciences?

PMID: 31460519 [PubMed]

PMCID: PMC6711478

EC Pharmacology and Toxicology
Is it a Prime Time for AI-powered Virtual Drug Screening?

PMID: 30215059 [PubMed]

PMCID: PMC6133253

EC Psychology and Psychiatry
Analysis of Evidence for the Combination of Pro-dopamine Regulator (KB220PAM) and Naltrexone to Prevent Opioid Use Disorder Relapse.

PMID: 30417173 [PubMed]

PMCID: PMC6226033

EC Anaesthesia
Arrest Under Anesthesia - What was the Culprit? A Case Report.

PMID: 30264037 [PubMed]

PMCID: PMC6155992

EC Orthopaedics
Distraction Implantation. A New Technique in Total Joint Arthroplasty and Direct Skeletal Attachment.

PMID: 30198026 [PubMed]

PMCID: PMC6124505

EC Pulmonology and Respiratory Medicine
Prevalence and factors associated with self-reported chronic obstructive pulmonary disease among adults aged 40-79: the National Health and Nutrition Examination Survey (NHANES) 2007-2012.

PMID: 30294723 [PubMed]

PMCID: PMC6169793

EC Dental Science
Important Dental Fiber-Reinforced Composite Molding Compound Breakthroughs

PMID: 29285526 [PubMed]

PMCID: PMC5743211

EC Microbiology
Prevalence of Intestinal Parasites Among HIV Infected and HIV Uninfected Patients Treated at the 1o De Maio Health Centre in Maputo, Mozambique

PMID: 29911204 [PubMed]

PMCID: PMC5999047

EC Microbiology
Macrophages and the Viral Dissemination Super Highway

PMID: 26949751 [PubMed]

PMCID: PMC4774560

EC Microbiology
The Microbiome, Antibiotics, and Health of the Pediatric Population.

PMID: 27390782 [PubMed]

PMCID: PMC4933318

EC Microbiology
Reactive Oxygen Species in HIV Infection

PMID: 28580453 [PubMed]

PMCID: PMC5450819

EC Microbiology
A Review of the CD4 T Cell Contribution to Lung Infection, Inflammation and Repair with a Focus on Wheeze and Asthma in the Pediatric Population

PMID: 26280024 [PubMed]

PMCID: PMC4533840

EC Neurology
Identifying Key Symptoms Differentiating Myalgic Encephalomyelitis and Chronic Fatigue Syndrome from Multiple Sclerosis

PMID: 28066845 [PubMed]

PMCID: PMC5214344

EC Pharmacology and Toxicology
Paradigm Shift is the Normal State of Pharmacology

PMID: 28936490 [PubMed]

PMCID: PMC5604476

EC Neurology
Examining those Meeting IOM Criteria Versus IOM Plus Fibromyalgia

PMID: 28713879 [PubMed]

PMCID: PMC5510658

EC Neurology
Unilateral Frontosphenoid Craniosynostosis: Case Report and a Review of the Literature

PMID: 28133641 [PubMed]

PMCID: PMC5267489

EC Ophthalmology
OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

PMID: 29333536 [PubMed]

PMCID: PMC5766278

EC Neurology
Longer Duration of Downslope Treadmill Walking Induces Depression of H-Reflexes Measured during Standing and Walking.

PMID: 31032493 [PubMed]

PMCID: PMC6483108

EC Microbiology
Onchocerciasis in Mozambique: An Unknown Condition for Health Professionals.

PMID: 30957099 [PubMed]

PMCID: PMC6448571

EC Nutrition
Food Insecurity among Households with and without Podoconiosis in East and West Gojjam, Ethiopia.

PMID: 30101228 [PubMed]

PMCID: PMC6086333

EC Ophthalmology
REVIEW. +2 to +3 D. Reading Glasses to Prevent Myopia.

PMID: 31080964 [PubMed]

PMCID: PMC6508883

EC Gynaecology
Biomechanical Mapping of the Female Pelvic Floor: Uterine Prolapse Versus Normal Conditions.

PMID: 31093608 [PubMed]

PMCID: PMC6513001

EC Dental Science
Fiber-Reinforced Composites: A Breakthrough in Practical Clinical Applications with Advanced Wear Resistance for Dental Materials.

PMID: 31552397 [PubMed]

PMCID: PMC6758937

EC Microbiology
Neurocysticercosis in Child Bearing Women: An Overlooked Condition in Mozambique and a Potentially Missed Diagnosis in Women Presenting with Eclampsia.

PMID: 31681909 [PubMed]

PMCID: PMC6824723

EC Microbiology
Molecular Detection of Leptospira spp. in Rodents Trapped in the Mozambique Island City, Nampula Province, Mozambique.

PMID: 31681910 [PubMed]

PMCID: PMC6824726

EC Neurology
Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.

PMID: 31528859 [PubMed]

PMCID: PMC6746603

EC Psychology and Psychiatry
Can Chronic Consumption of Caffeine by Increasing D2/D3 Receptors Offer Benefit to Carriers of the DRD2 A1 Allele in Cocaine Abuse?

PMID: 31276119 [PubMed]

PMCID: PMC6604646

EC Anaesthesia
Real Time Locating Systems and sustainability of Perioperative Efficiency of Anesthesiologists.

PMID: 31406965 [PubMed]

PMCID: PMC6690616

EC Pharmacology and Toxicology
A Pilot STEM Curriculum Designed to Teach High School Students Concepts in Biochemical Engineering and Pharmacology.

PMID: 31517314 [PubMed]

PMCID: PMC6741290

EC Pharmacology and Toxicology
Toxic Mechanisms Underlying Motor Activity Changes Induced by a Mixture of Lead, Arsenic and Manganese.

PMID: 31633124 [PubMed]

PMCID: PMC6800226

EC Neurology
Research Volunteers' Attitudes Toward Chronic Fatigue Syndrome and Myalgic Encephalomyelitis.

PMID: 29662969 [PubMed]

PMCID: PMC5898812

EC Pharmacology and Toxicology
Hyperbaric Oxygen Therapy for Alzheimer's Disease.

PMID: 30215058 [PubMed]

PMCID: PMC6133268

News and Events

March Issue Release

We always feel pleasure to share our updates with you all. Here, notifying you that we have successfully released the March issue of respective journals and can be viewed in the current issue pages.

Submission Deadline for April Issue

Ecronicon delightfully welcomes all the authors around the globe for effective collaboration with an article submission for the April issue of respective journals. Submissions are accepted on/before March 18, 2021.

Certificate of Publication

Ecronicon honors with a "Publication Certificate" to the corresponding author by including the names of co-authors as a token of appreciation for publishing the work with our respective journals.

Best Article of the Issue

Editors of respective journals will always be very much interested in electing one Best Article after each issue release. The authors of the selected article will be honored with a "Best Article of the Issue" certificate.

Certifying for Review

Ecronicon certifies the Editors for their first review done towards the assigned article of the respective journals.

Latest Articles

The latest articles will be updated immediately on the articles in press page of the respective journals.

Immediate Assistance

The prime motto of this team is to clarify all the queries without any delay or hesitation to avoid the inconvenience. For immediate assistance on your queries please don't hesitate to drop an email to