Research Article
Volume 16 Issue 10 - 2020
Bioelectricity Generation Using Marine Sediment and Cow Dung
BO Uba1*, KN Obidike1, CU Dokubo2 and ID Nnaodi1
1Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria
2Department of Science Laboratory Technology, Delta State Polytechnic Ogwashi - Uku, Delta State, Nigeria
*Corresponding Author: BO Uba, Lecturer, Department of Microbiology, Chukwuemeka Odumegwu Ojukwu University, Anambra State, Nigeria.
Received: June 18, 2020; Published: September 10, 2020




Abstract

In this study, the aim is to determine the application of marine waste and sediment mixed with cow dung as the substrate for generation of electricity by means of microbial fuel cell technology. The waste water sample and sediments were collected from Abonema water site at Port Harcourt Rivers State. The method employed for isolation, characterization and identification of electrogenic microbes involves the isolation on Nutrient agar, microscopy, biochemical tests (Analytical Profile Index (API) kit) and comparison with Bergey’ Manual of Determinative Bacteriology. The electrogenic organisms isolated include: Pseudomonas aeruginosa, Corynebacterium sp.,and Lactobacillus casei. Three (3) MFCs were fabricated with 1L transparent plastic for the cathode and anode chambers containing graphite electrode and PVC pipe salt bridge. The experiment was performed at room temperature with continuous monitoring and readings were taken after 24h of inoculation. The method employed for the multimetric measurement and physicochemical parameters involves voltage, current, power density, temperature, pH, conductivity; total dissolved solid (TDS) and total organic carbon (TOC). The method involved for biofilm formation and development involve coverslip overlay microscopic techniques. The result showed that MFC setup 1 generated the highest voltage and current of 391 mV and 126 mA. The pH and temperature of the MFC setups revealed slight acidicity to neutrality as well as moderate temperatures. There was biofilm development from 24h to 120h. Statistically, there is significant difference (P < 0.05) between the test sample and the control in all the parameters measured. This study revealed that the microbial fuel cell fabricated with the substrates above has a higher voltage and current productivity than the positive and negative control.

Keywords: Bacteria; Bioelectricity; Marine Ecosystem; Microbial Fuel Cell

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Citation: BO Uba., et al. “Bioelectricity Generation Using Marine Sediment and Cow Dung”. EC Microbiology 16.10 (2020): 01-12.

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