Research Protocol
Volume 16 Issue 07 - 2020
Biological and Genetic Characteristics of the Cholera Bacteriophage Rostov 7
NE Gaevskaya*, MP Pogozhova, AS Vodopyanov, RV Pisanov, LV Romanova, AO Anoprienko, SN Golovin and AV Tyurina
Rostov-on-Don Plague Control Research Institute of Rospotrebnadzor, Rostov-on-Don, Russian Federation
*Corresponding Author: NE Gaevskaya, Rostov-on-Don Plague Control Research Institute of Rospotrebnadzor, Rostov-on-Don, Russian Federation.
Received: February 20, 2020; Published: June 03, 2020


Currently а variety of genes encoding integrases, antibiotic resistance, and toxins had been identified which may be inserted into the phage genome. The study of isolated phages for the presence of these genes are highly relevant. The most informative method for identifying genetic determinants of pathogenicity factors and integrase is genome sequencing. The aim of our study was to study not only the biology of the cholera bacteriophage Vibrio phage Rostov 7, but also to conduct whole genome sequencing with a further assessment of the prospects for its practical use. The presence or absence of genetic determinants of resistance factors, toxins and integrase was checked with the help of the database created by us and the software developed, named “Phage Analyzer”. The morphology of the cholera bacteriophage Rostov 7 corpuscles was determined by means of electron microscopy, and his specificity was confirmed. On the lawn of the indicator culture, the bacteriophage Rostov 7 forms transparent negative colonies with a diameter of 1.0 - 1.5 mm. Analysis of the nucleotide sequences revealed that its genome size is 45903 bp with the total number of ORF 35, genes characteristic of caudate phages were also found. The bacteriophage Rostov 7 was shown to have a separate position and to be unique. Two integrases were found in the genome structure (YP_009043902.1 and YP_009043892.1), therefore, the use of phage in prophylactic or therapeutic preparations is excluded, since it is moderate. Genetic determinants of resistance factors and toxins were not detected. At the moment the bacteriophage is actively used in the experimental work of the laboratory. Vibrio phage Rostov 7 can be successfully used to construct diagnostic products. The complete genomic sequence of Vibrio phage Rostov 7 is deposited in the international database NCBI GenBank under the accession number MK575466.1.

Keywords: Vibrio Phage Rostov 7; Myoviridae; Cholera; Sequencing; Bacteriophage


  1. Ilyina TS. “The mechanism of horizontal gene transfer: the role of bacteriophages and integrons in the evolution of pathogenic bacteria”. Molecular Genetics, Microbiology and Virology 4 (2003): 3-10. 
  2. Katter E and Sulakvelidze A. “Bakteriofagi: biologiya i prakticheskoe primenenie [Bacteriophages: biology and practical application]”. A.V. Letarov (edition). Moscow: "Scientific World" Publication (2012): 640. 
  3. Shlegel G. “Obshchaya mikrobiologiya [General Microbiology]”. Moscow (1987): 142.
  4. Groth АС and Calos МР. “Phage Integrases: Biology and Applications”. Journal of Molecular Biology 335.3 (2004): 667-678. 
  5. Adams MH. “Bacteriophages”. New York, N.Y.: Inter science Publishers (1959): 620.
  6. Gabrilovich IM. “Prakticheskoe posobie po bakteriofagii [Practical guide to bacteriophage]”. Minsk, (1968): 10. 
  7. Maniatis T., et al. “Metody geneticheskoi inzhenerii: Molekulyarnoe klonirovanie [Genetic engineering techniques: Molecular cloning]”. Moscow (1984): 458. 
  8. Yamamoto KR., et al. “Rapid bacterio-phage sedimentation in the presence of polyethylene glycol and its application tolarge-scale virus purification”. Virology 40.3 (1970): 734-744. 
  9. Andrews S. “FastQC: a quality control tool for high throughput sequence data” (2010). 
  10. Bolger AM., et al. “Trimmomatic: A flexible trimmer for illumina sequence data”. Bioinformatics 30.15 (2014): 2114-2120. 
  11. Song L., et al. “Lighter: fast and memory-efficient sequencing error correction without counting”. Genome Biology 15.11 (2014): 509.
  12. Bankevich A., et al. “SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing”. Journal of Computational Biology 19.5 (2012): 455-477. 
Citation: NE Gaevskaya., et al. “Biological and Genetic Characteristics of the Cholera Bacteriophage Rostov 7”. EC Microbiology 16.7 (2020): 01-05.

PubMed Indexed Article

EC Pharmacology and Toxicology
LC-UV-MS and MS/MS Characterize Glutathione Reactivity with Different Isomers (2,2' and 2,4' vs. 4,4') of Methylene Diphenyl-Diisocyanate.

PMID: 31143884 [PubMed]

PMCID: PMC6536005

EC Pharmacology and Toxicology
Alzheimer's Pathogenesis, Metal-Mediated Redox Stress, and Potential Nanotheranostics.

PMID: 31565701 [PubMed]

PMCID: PMC6764777

EC Neurology
Differences in Rate of Cognitive Decline and Caregiver Burden between Alzheimer's Disease and Vascular Dementia: a Retrospective Study.

PMID: 27747317 [PubMed]

PMCID: PMC5065347

EC Pharmacology and Toxicology
Will Blockchain Technology Transform Healthcare and Biomedical Sciences?

PMID: 31460519 [PubMed]

PMCID: PMC6711478

EC Pharmacology and Toxicology
Is it a Prime Time for AI-powered Virtual Drug Screening?

PMID: 30215059 [PubMed]

PMCID: PMC6133253

EC Psychology and Psychiatry
Analysis of Evidence for the Combination of Pro-dopamine Regulator (KB220PAM) and Naltrexone to Prevent Opioid Use Disorder Relapse.

PMID: 30417173 [PubMed]

PMCID: PMC6226033

EC Anaesthesia
Arrest Under Anesthesia - What was the Culprit? A Case Report.

PMID: 30264037 [PubMed]

PMCID: PMC6155992

EC Orthopaedics
Distraction Implantation. A New Technique in Total Joint Arthroplasty and Direct Skeletal Attachment.

PMID: 30198026 [PubMed]

PMCID: PMC6124505

EC Pulmonology and Respiratory Medicine
Prevalence and factors associated with self-reported chronic obstructive pulmonary disease among adults aged 40-79: the National Health and Nutrition Examination Survey (NHANES) 2007-2012.

PMID: 30294723 [PubMed]

PMCID: PMC6169793

EC Dental Science
Important Dental Fiber-Reinforced Composite Molding Compound Breakthroughs

PMID: 29285526 [PubMed]

PMCID: PMC5743211

EC Microbiology
Prevalence of Intestinal Parasites Among HIV Infected and HIV Uninfected Patients Treated at the 1o De Maio Health Centre in Maputo, Mozambique

PMID: 29911204 [PubMed]

PMCID: PMC5999047

EC Microbiology
Macrophages and the Viral Dissemination Super Highway

PMID: 26949751 [PubMed]

PMCID: PMC4774560

EC Microbiology
The Microbiome, Antibiotics, and Health of the Pediatric Population.

PMID: 27390782 [PubMed]

PMCID: PMC4933318

EC Microbiology
Reactive Oxygen Species in HIV Infection

PMID: 28580453 [PubMed]

PMCID: PMC5450819

EC Microbiology
A Review of the CD4 T Cell Contribution to Lung Infection, Inflammation and Repair with a Focus on Wheeze and Asthma in the Pediatric Population

PMID: 26280024 [PubMed]

PMCID: PMC4533840

EC Neurology
Identifying Key Symptoms Differentiating Myalgic Encephalomyelitis and Chronic Fatigue Syndrome from Multiple Sclerosis

PMID: 28066845 [PubMed]

PMCID: PMC5214344

EC Pharmacology and Toxicology
Paradigm Shift is the Normal State of Pharmacology

PMID: 28936490 [PubMed]

PMCID: PMC5604476

EC Neurology
Examining those Meeting IOM Criteria Versus IOM Plus Fibromyalgia

PMID: 28713879 [PubMed]

PMCID: PMC5510658

EC Neurology
Unilateral Frontosphenoid Craniosynostosis: Case Report and a Review of the Literature

PMID: 28133641 [PubMed]

PMCID: PMC5267489

EC Ophthalmology
OCT-Angiography for Non-Invasive Monitoring of Neuronal and Vascular Structure in Mouse Retina: Implication for Characterization of Retinal Neurovascular Coupling

PMID: 29333536 [PubMed]

PMCID: PMC5766278

EC Neurology
Longer Duration of Downslope Treadmill Walking Induces Depression of H-Reflexes Measured during Standing and Walking.

PMID: 31032493 [PubMed]

PMCID: PMC6483108

EC Microbiology
Onchocerciasis in Mozambique: An Unknown Condition for Health Professionals.

PMID: 30957099 [PubMed]

PMCID: PMC6448571

EC Nutrition
Food Insecurity among Households with and without Podoconiosis in East and West Gojjam, Ethiopia.

PMID: 30101228 [PubMed]

PMCID: PMC6086333

EC Ophthalmology
REVIEW. +2 to +3 D. Reading Glasses to Prevent Myopia.

PMID: 31080964 [PubMed]

PMCID: PMC6508883

EC Gynaecology
Biomechanical Mapping of the Female Pelvic Floor: Uterine Prolapse Versus Normal Conditions.

PMID: 31093608 [PubMed]

PMCID: PMC6513001

EC Dental Science
Fiber-Reinforced Composites: A Breakthrough in Practical Clinical Applications with Advanced Wear Resistance for Dental Materials.

PMID: 31552397 [PubMed]

PMCID: PMC6758937

EC Microbiology
Neurocysticercosis in Child Bearing Women: An Overlooked Condition in Mozambique and a Potentially Missed Diagnosis in Women Presenting with Eclampsia.

PMID: 31681909 [PubMed]

PMCID: PMC6824723

EC Microbiology
Molecular Detection of Leptospira spp. in Rodents Trapped in the Mozambique Island City, Nampula Province, Mozambique.

PMID: 31681910 [PubMed]

PMCID: PMC6824726

EC Neurology
Endoplasmic Reticulum-Mitochondrial Cross-Talk in Neurodegenerative and Eye Diseases.

PMID: 31528859 [PubMed]

PMCID: PMC6746603

EC Psychology and Psychiatry
Can Chronic Consumption of Caffeine by Increasing D2/D3 Receptors Offer Benefit to Carriers of the DRD2 A1 Allele in Cocaine Abuse?

PMID: 31276119 [PubMed]

PMCID: PMC6604646

EC Anaesthesia
Real Time Locating Systems and sustainability of Perioperative Efficiency of Anesthesiologists.

PMID: 31406965 [PubMed]

PMCID: PMC6690616

EC Pharmacology and Toxicology
A Pilot STEM Curriculum Designed to Teach High School Students Concepts in Biochemical Engineering and Pharmacology.

PMID: 31517314 [PubMed]

PMCID: PMC6741290

EC Pharmacology and Toxicology
Toxic Mechanisms Underlying Motor Activity Changes Induced by a Mixture of Lead, Arsenic and Manganese.

PMID: 31633124 [PubMed]

PMCID: PMC6800226

EC Neurology
Research Volunteers' Attitudes Toward Chronic Fatigue Syndrome and Myalgic Encephalomyelitis.

PMID: 29662969 [PubMed]

PMCID: PMC5898812

EC Pharmacology and Toxicology
Hyperbaric Oxygen Therapy for Alzheimer's Disease.

PMID: 30215058 [PubMed]

PMCID: PMC6133268

News and Events

August Issue Release

We always feel pleasure to share our updates with you all. Here, notifying you that we have successfully released the August issue of respective journals and can be viewed in the current issue pages.

Submission Deadline for September Issue

Ecronicon delightfully welcomes all the authors around the globe for effective collaboration with an article submission for the September issue of respective journals. Submissions are accepted on/before August 15, 2020.

Certificate of Publication

Ecronicon honors with a "Publication Certificate" to the corresponding author by including the names of co-authors as a token of appreciation for publishing the work with our respective journals.

Best Article of the Issue

Editors of respective journals will always be very much interested in electing one Best Article after each issue release. The authors of the selected article will be honored with a "Best Article of the Issue" certificate.

Certifying for Review

Ecronicon certifies the Editors for their first review done towards the assigned article of the respective journals.

Latest Articles

The latest articles will be updated immediately on the articles in press page of the respective journals.

Immediate Assistance

The prime motto of this team is to clarify all the queries without any delay or hesitation to avoid the inconvenience. For immediate assistance on your queries please don't hesitate to drop an email to