Research Article
Volume 16 Issue 6 - 2020
Antibiotic Sensitivity Test for Environmental Bacteria (Brevibacillus brevis, Bacillus coagulans, Enterococcus raffinosus, and Macrococcus caseolyticus)
Dareen El Shareef Ahmed1*, Hussein Yassine2, Hana Gadalla3 and Ana Janic4
1M.S. in Microbiology, Faculty of Pharmacy, Benghazi University, Benghazi, Libya
2Dental Student, School of Dentistry, University of Detroit Mercy, Detroit, Michigan, USA
3Private Practice Periodontist Metro Detroit Area, USA
4Assistant Professor, Division of Clinical Dentistry, School of Dentistry, University of Detroit Mercy, Detroit, Michigan, USA
*Corresponding Author: Dareen El Shareef Ahmed, M.S. in Microbiology, Faculty of Pharmacy, Benghazi University, Benghazi, Libya.
Received: February 12, 2020; Published: May 21, 2020


Four gram positive bacteria were isolated from the aerosol of Al Marj City from December 2012 through March 2013, because there was no research related to this subject previously completed. The four bacteria evaluated were as follows: Brevibacillus brevis, Bacillus coagulans, Enterococcus raffinosus and Macrococcus caseolyticus. They were screened for sensitivity to twelve antibiotics by the Bauer disc diffusion method. The highest sensitivity was recorded to Chloramphenicol, Ciprofloxacin, and Amikacin. High resistance was observed for Macrococcus caseolyticus in Colistin sulphate, Ampicillin, Oxacillin, Cephalexin, Cefoxitin and Augmentin. Highest sensitivity recorded for Macrococcus caseolyticus with Chloramphenicol, Ciprofloxacin, Erythromycin, Gentamycin, Tobramycin and Amikacin. High resistance was observed in Colistin sulphate, Erythromycin, Oxacillin, Cephalexin and Cefoxitin and higher sensitivity performed for Brevibacillus brevis by Chloramphenicol, Ciprofloxacin, Augmentin and Amikacin. High resistance was observed in Oxacillin, Cephalexin and Cefoxitin and higher sensitivity performed for Bacillus coagulans by Chloramphenicol, Ciprofloxacin, Augmentin, Erythromycin and Amikacin. Of these, the highest sensitivity was recorded with Augmentin. High resistance was recorded for Colistin sulphate. By means of antibiotic sensitivity test, highest resistance was recorded to Brevibacillus brevis and the least resistance was recorded to Macrococcus caseolyticus. The combination of antibiotic Amoxicillin/Clavulanic acid (Augmentin) was found to be the most effective antibiotic with highest sensitivity recorded for three environmental bacteria, which are Brevibacillus brevis, Bacillus coagulans and Enterococcus raffinosus.

Keywords: Brevibacillus brevis; Bacillus coagulans; Enterococcus raffinosus; Macrococcus caseolyticus


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Citation: Dareen El Shareef Ahmed.,et al. “Antibiotic Sensitivity Test for Environmental Bacteria (Brevibacillus brevis, Bacillus coagulans, Enterococcus raffinosus, and Macrococcus caseolyticus)”. EC Microbiology 16.6 (2020): 72-103.

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