Research Article
Volume 16 Issue 7 - 2020
Anti-inflammatory Effects of Low Level Laser Therapy and Streptococcus thermophilus on Wound Assessed Histomorphologically: A Comparative Study in Diabetic Rat’s Model
Muhammad Asif1, Devi Kumari2, Muhammad Rafique2, Hina Khan2*, Asad Raza Jiskani3 and Fouzia Kirmani2
1Department of Rehabilitation Sciences, Isra University, Karachi Campus, Pakistan
2Department of Anatomy, Al-Tibri Medical College and Hospital, Isra University, Karachi Campus, Pakistan
3Department of Community Medicine, Al-Tibri Medical College and Hospital, Isra University, Karachi Campus, Pakistan
*Corresponding Author: Hina Khan, Associate Professor of Anatomy, Deputy Director Research and PG Affairs, Al-Tibri Medical College and Hospital, Isra University, Karachi Campus, Pakistan
Received: March 16, 2020; Published: June 30, 2020




Abstract

Background and Objectives: Worldwide, diabetic wound is a major concern. In diabetic wounds, medical professionals require excellent therapy choice with less devastating consequences. Laser therapy and Streptococcus thermophilus are potent therapeutic agents. The topical effects of laser and Streptococcus thermophilus on the inflammatory phase of diabetic injury are compared in this research.

Material and Methods: The total 18 rats were divided randomly into three groups and six rats were placed into each group. Diabetes mellitus was induced in rats by administration of Alloxan intraperitoneally. After induction of diabetes, wounds were created in all rats. Control group received normal saline, group B irradiated with 10 J/cm2 for 30 sec with Low Level Laser Therapy, group C was given 1 ml Streptococcus thermophilus application containing 1010 to 1011 CFU/ml organisms topically. Neutrophils, lymphocytes and macrophages were counted on the 3rd and 7th day of therapy to determine the inflammatory status of diabetic wound.

Results: In comparison to Streptococcus thermophilus and normal saline with p values < 0.02 the rats were handled with Low level laser therapy showed a significant reduction in the number of neutrophil counts with increased number of macrophages and lymphocytes.

Conclusion: In diabetic wounds, both low level laser therapy and Streptococcus thermophilus exerted their anti-inflammatory effects; however, low level laser therapy showed greater preceding anti-inflammatory impacts as compared with Streptococcus thermophilus.

Keywords: Diabetes Mellitus; Low Level Laser Therapy (LLLT); Streptococcus thermophilus; Neutrophils; Lymphocytes; Macrophages

References

  1. Buchleitner AM., et al. “Perioperative glycaemic control for diabetic patients undergoing surgery”. Cochrane Database of Systematic Reviews9 (2012): CD007315.
  2. Oviedo-Socarrás T., et al. “Diabetes alters inflammation, angiogenesis, and fibrogenesis in intraperitoneal implants in rats”. Microvascular Research 93 (2014): 23-29.
  3. Peppa M., et al. “Advanced glycoxidation products and impaired diabetic wound healing”. Wound Repair and Regeneration4 (2009): 461-472.
  4. Wild S., et al. “Global prevalence of diabetes: estimates for the year 2000 and projections for 2030”. Diabetes Care5 (2004): 1047-1053.
  5. França CM., et al. “Effect of laser therapy on skeletal muscle repair process in diabetic rats”. Lasers in Medical Scienc 5 (2013): 1331-1338.
  6. Kajagar BM., et al. “Efficacy of low level laser therapy on wound healing in patients with chronic diabetic foot ulcers-a randomised control trial”. Indian Journal of Surgery5 (2012): 359-363.
  7. Núñez SC., et al. “The influence of red laser irradiation timeline on burn healing in rats”. Lasers in Medical Science2 (2013): 633-641.
  8. Houreld NN. “Shedding light on a new treatment for diabetic wound healing: a review on phototherapy”. The Scientific World Journal (2014): 398412-398412.
  9. Chung H., et al. “The nuts and bolts of low-level laser (light) therapy”. Annals of Biomedical Engineering2 (2012): 516-533.
  10. Alves AC., et al. “ Effect of low-level laser therapy on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation”. Arthritis Research and Therapy5 (2013): 116-127.
  11. Fukuda TY., et al. “Infrared low-level diode laser on inflammatory process modulation in mice: pro-and anti-inflammatory cytokines”. Lasers in Medical Science5 (2013): 1305-1313.
  12. Junker JP., et al. “Assessing quality of healing in skin: review of available methods and devices”. Wound Repair and Regeneration 22 (2014): 2-10.
  13. Nteleki B and Houreld NN. “The use of phototherapy in the treatment of diabetic ulcers”. Journal of Endocrinology, Metabolism and Diabetes of South Africa 3 (2012): 128-132.
  14. Mizutani Y., et al. “Ceramide biosynthesis in keratinocyte and its role in skin function”. Biochimie 6 (2009): 784-790.
  15. Di Marzio L., et al. “Effect of the Lactic Acid Bacterium Streptococcus thermophilus on Ceramide Levels in Human Keratinocytes In Vitro and Stratum Corneum In Vivo”. Journal of Investigative Dermatology1 (1999): 98-106.
  16. Di Marzio L., et al. “Effect of the lactic acid bacterium Streptococcus thermophilus on stratum corneum ceramide levels and signs and symptoms of atopic dermatitis patients”. Experimental Dermatology5 (2003): 615-620.
  17. Arany PR. “Craniofacial wound healing with photobiomodulation therapy: new insights and current challenges”. Journal of Dental Research9 (2016): 977-984.
  18. Piva JA., et al. “Effect of low-level laser therapy on the initial stages of tissue repair: basic principles”. Anais Brasileiros De Dermatologia5 (2011): 947-954.
  19. Ma W., et al. “Complicated Effect of He-Ne Laser Therapy on Pro-/Anti-Inflammatory Cytokines from Serum in Rats”. Animal and Veterinary Sciences 5 (2018): 88-94.
  20. Albertini R., et al. “Cytokine mRNA expression is decreased in the subplantar muscle of rat paw subjected to carrageenan-induced inflammation after low-level laser therapy”. Photomedicine and Laser Surgery1 (2008): 19-24.
  21. Alves AN., et al. “Effects of low-level laser therapy on skeletal muscle repair: a systematic review”. American Journal of Physical Medicine and Rehabilitation12 (2014): 1073-1085.
  22. Del Carmen S., et al. “Evaluation of a Streptococcus thermophilus strain with innate anti-inflammatory properties as a vehicle for IL-10 cDNA delivery in an acute colitis model”. Cytokine 2 (2015): 177-183.
  23. Del Carmen S., et al. “Genetically engineered immunomodulatory Streptococcus thermophilus strains producing antioxidant enzymes exhibit enhanced anti-inflammatory activities”. Applied and Environmental Microbiology 3 (2014): 869-877.
  24. De Loura Santana C., et al. “Tissue Responses to Postoperative Laser Therapy in Diabetic Rats Submitted to Excisional Wounds”. PLoS ONE4 (2015): 1-13.
Citation: Hina Khan., et al.. “Anti-inflammatory Effects of Low Level Laser Therapy and Streptococcus thermophilus on Wound Assessed Histomorphologically: A Comparative Study in Diabetic Rat’s Model”. EC Microbiology 16.7 (2020): 44-52.

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