Research Article
Volume 16 Issue 7 - 2020
Anti-inflammatory Effects of Low Level Laser Therapy and Streptococcus thermophilus on Wound Assessed Histomorphologically: A Comparative Study in Diabetic Rat’s Model
Muhammad Asif1, Devi Kumari2, Muhammad Rafique2, Hina Khan2*, Asad Raza Jiskani3 and Fouzia Kirmani2
1Department of Rehabilitation Sciences, Isra University, Karachi Campus, Pakistan
2Department of Anatomy, Al-Tibri Medical College and Hospital, Isra University, Karachi Campus, Pakistan
3Department of Community Medicine, Al-Tibri Medical College and Hospital, Isra University, Karachi Campus, Pakistan
*Corresponding Author: Hina Khan, Associate Professor of Anatomy, Deputy Director Research and PG Affairs, Al-Tibri Medical College and Hospital, Isra University, Karachi Campus, Pakistan
Received: March 16, 2020; Published: June 30, 2020


Background and Objectives: Worldwide, diabetic wound is a major concern. In diabetic wounds, medical professionals require excellent therapy choice with less devastating consequences. Laser therapy and Streptococcus thermophilus are potent therapeutic agents. The topical effects of laser and Streptococcus thermophilus on the inflammatory phase of diabetic injury are compared in this research.

Material and Methods: The total 18 rats were divided randomly into three groups and six rats were placed into each group. Diabetes mellitus was induced in rats by administration of Alloxan intraperitoneally. After induction of diabetes, wounds were created in all rats. Control group received normal saline, group B irradiated with 10 J/cm2 for 30 sec with Low Level Laser Therapy, group C was given 1 ml Streptococcus thermophilus application containing 1010 to 1011 CFU/ml organisms topically. Neutrophils, lymphocytes and macrophages were counted on the 3rd and 7th day of therapy to determine the inflammatory status of diabetic wound.

Results: In comparison to Streptococcus thermophilus and normal saline with p values < 0.02 the rats were handled with Low level laser therapy showed a significant reduction in the number of neutrophil counts with increased number of macrophages and lymphocytes.

Conclusion: In diabetic wounds, both low level laser therapy and Streptococcus thermophilus exerted their anti-inflammatory effects; however, low level laser therapy showed greater preceding anti-inflammatory impacts as compared with Streptococcus thermophilus.

Keywords: Diabetes Mellitus; Low Level Laser Therapy (LLLT); Streptococcus thermophilus; Neutrophils; Lymphocytes; Macrophages


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Citation: Hina Khan., et al.. “Anti-inflammatory Effects of Low Level Laser Therapy and Streptococcus thermophilus on Wound Assessed Histomorphologically: A Comparative Study in Diabetic Rat’s Model”. EC Microbiology 16.7 (2020): 44-52.

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