Research Article
Volume 5 Issue 3 - 2020
Isolation, Identification and Antibiotic Sensitivity Test of Bacteria in Raw Cow Milk Obtained from Different Sources
Mariam Jamila1, Md Zulfekar Ali2, Shaharin Sultana3, Md Ariful Islam1 and Mst Minara Khatun1*
1Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh
2Animal Health Research Division, Bangladesh Livestock Research Institute (BLRI), Bangladesh
3Department of Livestock Services (DLS), Ministry of Fisheries and Livestock, Bangladesh
*Corresponding Author: Mst Minara Khatun, Department of Microbiology and Hygiene, Bangladesh Agricultural University, Mymensingh, Bangladesh.
Received: February 04, 2020; Published: February 29, 2020




Abstract

The study was conducted to isolate, identify the bacteria, to know the sources of contamination of milk and antibiotic sensitivity of bacteria obtained from various sources. A total of 50 samples were collected from different sources such as hand wash of milkers’, udder wash, utensils wash, raw cow milk and environment (air). All these samples were analyzed by culturing in different media such as Salmonella-Shigella agar, Eosin Methylene Blue agar, Mannitol Salt Agar, Nutrient agar, Blood Agar, MacConkey agar. Biochemical tests were performed to identify the organism. Among 50 samples, 12 (24%) were E. coli. Similarly, 13 (26%) and 36 (72%) were found positive for Salmonella spp. and Staphylococcus spp. respectively. Results of antibiotic sensitivity test represent that, out of ten antibiotics Staphylococcus sp. were sensitive against 9 antibiotics such as Chloramphenicol, Gentamicin, Kanamycin, Sulphamethoxazole, Ampicillin, Amoxicillin, Ciprofloxacin, Tetracycline, Erythromycin and resistant against Nalidixic acid. Salmonella spwas sensitive to 6 antibiotics such as Chloramphenicol, Gentamicin, Kanamycin, Sulphamethoxazole, Ampicillin, Amoxicillin but resistant against Ciprofloxacin, Tetracycline, Erythromycin and Nalidixic acid. Whereas, E. coli were sensitive against 7 antibiotics such as Chloramphenicol, Gentamicin, Kanamycin, Sulphamethoxazole, Ciprofloxacin, Tetracycline and Nalidixic acid but resistant against Ampicillin, Erythromycin and intermediate to Ampicillin. In this study Chloramphenicol, Gentamicin, Kanamycin and Sulphamethoxazole were sensitive against the three isolated bacteria. Among all isolates Salmonella showing resistance to at least more than three antibiotic classes and E. coli were showing multidrug resistant. Data of this study suggested that raw milk contaminated with multidrug resistant bacteria which may cause public health hazard.

Keywords: Prevalence; Raw Milk; Bacteria; Antibiotic Sensitivity

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Citation: Mst Minara Khatun., et al. “Isolation, Identification and Antibiotic Sensitivity Test of Bacteria in Raw Cow Milk Obtained from Different Sources”. EC Microbiology 16.4 (2020): 32-41.

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