Research Article
Volume 10 Issue 6 - 2021
A New Oral Supplementation Based on the Ataxia-Telangiectasia-Mutated Repair Pathway Enhancement Reduces Sperm DNA Damage and Improves Semen Parameters
Sandra Lara-Cerrillo1, Josu Franco Iriarte2, María Velasco Álvarez3, Estefanía Rodríguez Ferradas3, Álvaro Vives Suñé4, Marta Ballester Ferrer4, Tania Lacruz-Ruiz1, Candela Rosado-Iglesias1, Ana Raquel Jiménez Macedo5, Joan Carrera Rotllan5 and Agustín García-Peiró1*
1CIMAB, Barcelona Male Infertility Centre, Research and Development Department, Sant Quirze del Vallès, Barcelona, Spain
2Hospital Ruber Internacional, Assisted Reproduction Department, Madrid, Spain
3Instituto Vasco de Fertilidad, Grupo IVF Life San Sebastián, Assisted Reproduction Department, Donostia, Guipuzkoa, Spain
4Hospital Universitario Dexeus, Department of Obstetrics, Gynaecology and Reproduction, Reproductive Medicine Unit, Barcelona, Spain
5Unitat d’Endocrinologia Ginecològica, Assisted Reproduction Department, Vic, Barcelona, Spain
*Corresponding Author: Agustín García Peiró, CIMAB, Barcelona Male Infertility Centre, Research and Development Department, Sant Quirze del Vallès, Barcelona, Spain.
Received: April 30, 2021; Published: May 08, 2021




Abstract

Background: A male factor is present in half of infertile couples and the presence of sperm DNA damage has been shown to be a key factor in these cases. The presence of Double-Strand Breaks (DSB) could cause alternations in embryos’ development and recurrent miscarriage. DSB are naturally produced during male meiosis and the Ataxia-Telangiectasia-Mutated (ATM) repair pathway plays an essential role in its repair. However, defects in DNA repair or an excessive production of DNA breaks may lead to high DSB values in the ejaculate. Single-Strand Breaks (SSB) have an oxidative origin, therefore, antioxidant supplements showed to reduce its values. Unfortunately, there are no specific approaches to reduce DSB. Here it is proposed that the enhancement of DSB-repair-pathways could be an efficient strategy to improve sperm quality. For this purpose, new oral supplements containing Curcumin and Gingko Biloba extract have been tested based on their antioxidant properties and their ability to enhance the ATM repair pathway.

Objective: To test three different oral supplements regarding their capacity to reduce DSB values in affected males. SSB and semen parameters were also studied.

Methodology: This prospective and multicentric study tested two formulas containing different doses of Curcumin and Gingko Biloba extract that were administered to infertile males presenting high DSB values. A commercialized antioxidant not containing these substances was also tested. Sperm DNA damage and semen parameters were evaluated in 71 patients at baseline and after 13 weeks. DSB and SSB were measured using the CometAssay.

Results: The greatest reduction of DSB values was observed in Formula 1 containing the highest dose of Curcumin and Gingko Biloba extract (27.1%). Formula 2 and the Control group reduced 19.9% and 15.8%, respectively. Formula 1 also reduced SSB (19.5%). Regarding semen parameters, Formulas 1 and 2 increased sperm concentration, sperm count and progressive motility. The control group reduced these values.

Conclusion: Formula 1 is a new oral supplement designed to enhance the ATM repair pathway which significantly reduced DSB and SSB, and improved semen parameters. The prevention of sperm DNA damage through effective oral supplementation focused on improving DNA damage repair could be of great interest in patients affected by DSB.

Keywords: Double-Strand Breaks; Curcumin; Gingko Biloba Extract; Antioxidants; Sperm DNA Damage; Male Fertility

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Citation: Agustín García-Peiró., et al. “A New Oral Supplementation Based on the Ataxia-Telangiectasia-Mutated Repair Pathway Enhancement Reduces Sperm DNA Damage and Improves Semen Parameters”. EC Gynaecology 10.6 (2020): 27-39.

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