Research Article
Volume 9 Issue 9 - 2020
Regional Variations in Placental Protein Expression of Matrix Metalloproteinase-9 in Term Uncomplicated Pregnancy
Jayasri Basu*, Sara Oraee, Yingyi Wu, Diana M Encalada, Aruna Mishra and Magdy Mikhail
BronxCare Health System, Department of Obstetrics and Gynecology, Bronx, New York, USA
*Corresponding Author: Jayasri Basu, Director- Graduate Medical Education Department of OB/GYN, BronxCare Health System, Clinical Assistant Professor of Obstetrics and Gynecology and Reproductive Science Icahn, School of Medicine of Mount Sinai, Bronx, New York, USA.
Received:June 18, 2020; Published: August 05, 2020




Abstract

Objective: The present study explored whether placental expression of matrix metalloproteinase-9 (MMP-9) would vary between regions of the same term delivered placentas that were obtained from women with uncomplicated pregnancy.

Methods: A total of 27 term delivered placentas were collected from healthy women without any medical or pregnancy related complications. Each placenta was divided into four quadrants and MMP-9 protein expression was compared between the quadrants of each placenta. Direct Sandwich Enzyme-linked immunoassay (ELISA) method was used to determine chorionic villi MMP-9 protein expression of the tissue sections from each quadrant. Commercially available assay kits from R&D Systems, Minneapolis, MN; were used that used monoclonal antibody to human MMP-9 protein as the capture antibody.

Results: MMP-9 protein expressions of 108 chorionic villi samples obtained from four different quadrants of each of 27 normal placentas was comparable between the four quadrants. Chorionic villi MMP-9 protein expression did not differ between C-section and vaginal delivery groups. The protein expression was comparable between the studied ethnic groups. Maternal age was significantly but negatively correlated with gestational age in days (r2 = -0.201, p = 0.037).

Conclusion: The study underscores that even though each placenta may differ significantly in its structure and vasculature, MMP-9 protein expression at term is expressed equally throughout the placenta in normal healthy women.

Keywords: MMP-9; Term Placenta; Normal Human Pregnancy; Placental Quadrants; ELISA

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Citation: Jayasri Basu., et al. “Regional Variations in Placental Protein Expression of Matrix Metalloproteinase-9 in Term Uncomplicated Pregnancy”. EC Gynaecology 9.9 (2020): 26-33.

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