Research Article
Volume 9 Issue 3 - 2020
Prevalence and Characterization of High-Risk Human Papillomavirus Genotypes among a Group of Sex Workers in Ouagadougou, Burkina Faso
Régine Ilboudo1,3,4, Esther MA Traoré1,2, Théodora Mahoukèdè Zohoncon1,2,5, Rogomenoma Alice Ouédraogo1,2, Ina Marie Angèle Traore1,2, Prosper Bado1,2,Florencia Wendkuuni Djigma1,2, Dorcas Obiri-Yeboah6, Jean Sakandé4, Jacques Simpore1,2,5 and Charlemagne Ouedraogo1,3,4*
1Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, Burkina Faso
2Pietro Annigoni Biomolecular Research Centre (CERBA), Burkina Faso
3Obstetrician-gynecologist, UFR/SDS, University of Ouagadougou, Burkina Faso
4Yalgado Ouedraogo University Hospital (CHU/YO), Burkina Faso
5Faculty of Medicine, University Saint Thomas d’Aquin, Burkina Faso
6Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Ghana
*Corresponding Author: Charlemagne Ouedraogo, Laboratory of Molecular Biology and Genetics (LABIOGENE), University of Ouagadougou, Burkina Faso.
Received: December 05, 2019; Published: February 06, 2020


Background: Human papillomavirus (HPV) is the most common cause of cervical cancer worldwide. The aim of this study was to determine the prevalence and to characterize circulating high-risk HPV genotypes among female sex workers in Ouagadougou.

Patients and Methods: A total of 200 sex workers voluntarily agreed to take part in this study and swabs were taken from the endocervical canal for HPV detection. Real-time PCR was used for HPV genotype identification.

Results: Overall HPV carriage infection was 53%. One hundred six (106) sex workers were positive for at least one high-risk HPV genotype. Fourteen genotypes corresponding to 225 infections, were characterized: HPV 68 (14.6%), HPV 31 (12%), HPV 52 (9.3%), HPV 51 (8.9%), HPV 56 (7, 6%), HPV 66 (7.6%), HPV 58 (7.1%), HPV 35 (7.1%), HPV 39 (6.2%), HPV 18 (6.2%), HPV 45 (5.8%), HPV 59 (3.1%), HPV 16 (2.7%), HPV 33 (1.8%). Multiple infection (2 - 9 genotypes) was detected in 53.8% of infected sex workers. The number of sexual partners was statistically associated with carriage of HPV (p ≤ 0.01, OR = 2.000, 95% CI = 0.56 - 7.14), early intercourse was not.

Conclusion: The prevalence of HPV carriage in this study was high and associated with having a high number of multiple infections. Primary preventions such as sex education and vaccine use could significantly decrease the incidence of HPV infections.

Keywords: Sex-Workers; HPV; Genotypes; PCR


  1. Duport N. “Donnée épidemiologiques sur le cancer du col de l'utérus - Etat de connaissances – Actualisation”. Institut de Veille Sanitaire (2008): 30. 
  2. OMS OMdls. “La lutte contre le cancer du col de l'utérus”. Guide des pratiques essentielles-2nd édition. (Genève).
  3. Collins S. “High incidence of cervical human papillomavirus infection in women during their first sexual relationship”. British Journal of Obstetrics and Gynaecology 109.1 (2002): 96-98.
  4. Ly A. “Le cancer du col utérin: nouveaux vaccins, nouvelles perspectives?” African Journal of Cancer 1 (2009): 65-67.
  5. Schiffman M and Kjaer SK. “Chapter 2: Natural history of anogenital human papillomavirus infection and neoplasia”. Journal of the National Cancer Institute Monographs 31 (2003): 14-19.
  6. Munoz N., et al. “Epidemiologic classification of human papillomavirus types associated with cervical cancer”. The New England Journal of Medicine 348.6 (2003): 518-527.
  7. Ouedraogo CM., et al. “Epidemiology, characterization of genotypes of human papillomavirus in a population of women in Ouagadougou”. Journal of Gynecology Obstetrics and Reproduction Biology 40.7 (2011): 633-638.
  8. Kjaer SK., et al. “Human papillomavirus infection in Danish female sex workers. Decreasing prevalence with age despite continuously high sexual activity”. Sexually Transmitted Diseases 27.8 (2000): 438-445.
  9. Djigma FW., et al. “Prevalence and genotype characterization of human papillomaviruses among HIV-seropositive in Ouagadougou, Burkina Faso”. Acta Tropica 117.3 (2011): 202-206.
  10. Didelot-Rousseau MN., et al. “Human papillomavirus genotype distribution and cervical squamous intraepithelial lesions among high-risk women with and without HIV-1 infection in Burkina Faso”. British Journal of Cancer 95.3 (2006): 355-362.
  11. Marek E., et al. “Human papillomavirus infections among Hungarian female sex workers”. European Journal of Cancer Care 23.1 (2014): 65-75.
  12. Ouedraogo CM., et al. “Epidemiology and characterization of high-risk genotypes of human Papillomavirus in a population of sexually active adolescents in Ouagadougou”. Journal of Gynecology Obstetrics and Reproduction Biology 44.8 (2015): 715-722.
  13. Tse C and Capeau J. “Real time PCR methodology for quantification of nucleic acids”. Annales de biologie Clinique 61.3 (2003): 279-293.
  14. Latora V., et al. “Network of sexual contacts and sexually transmitted HIV infection in Burkina Faso”. Journal of Medical Virology 78.6 (2006): 724-729.
  15. Traore IM., et al. “Molecular Characterization of High-Risk Human Papillomavirus in Women in Bobo-Dioulasso, Burkina Faso”. BioMed Research International (2016): 7092583.
  16. Panatto D., et al. “Prevalence of human papillomavirus in young Italian women with normal cytology: how should we adapt the national vaccination policy?”. BMC Infectious Diseases 13 (2013): 575.
  17. Ramanakumar AV., et al. “Human papillomavirus (HPV) types 16, 18, 31, 45 DNA loads and HPV-16 integration in persistent and transient infections in young women”. BMC Infectious Diseases 10 (2010): 326.
  18. Rousseau MC., et al. “Predictors of cervical coinfection with multiple human papillomavirus types”. Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 12.10 (2003): 1029-1037.
  19. Clifford GM., et al. “Worldwide distribution of human papillomavirus types in cytologically normal women in the International Agency for Research on Cancer HPV prevalence surveys: a pooled analysis”. Lancet 366.9490 (2005): 991-998.
  20. Zohoncon TM., et al. “Prevalence of HPV High-Risk Genotypes in Three Cohorts of Women in Ouagadougou (Burkina Faso)”. Mediterranean Journal of Hematology and Infectious Diseases 5.1 (2013): e2013059.
  21. Chaturvedi AK., et al. “Human papillomavirus infection with multiple types: pattern of coinfection and risk of cervical disease”. The Journal of Infectious Diseases 203.7 (2011): 910-920.
  22. Winer RL., et al. “Genital human papillomavirus infection: incidence and risk factors in a cohort of female university students”. American Journal of Epidemiology 157 (2003): 218-226.
  23. Winer RL., et al. “Condom use and the risk of genital human papillomavirus infection in young women”. The New England Journal of Medicine 354 (2006): 2645-2654.
  24. Obiri-Yeboah D., et al. “Epidemiology of cervical human papillomavirus (HPV) infection and squamous intraepithelial lesions (SIL) a cohort of HIV-infected and uninfected Ghanaian women”. BMC Cancer 17.1 (2017): 688.
Citation: Charlemagne Ouedraogo., et al. “Prevalence and Characterization of High-Risk Human Papillomavirus Genotypes among a Group of Sex Workers in Ouagadougou, Burkina Faso”. EC Gynaecology 9.3 (2020): 01-09.

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