Research Article
Volume 4 Issue 2 - 2020
Global No-Flow Ischemia and Chemical Stress Condition Effects on D-3-hydroxybutyrate and Glucose Utilization in the Isolated Perfused Heart
Abdurazzaq Mohammed Sultan1*, Qusai A Sultan2, Abdulwahab H Bin Melaih2, Tareq H Al Kaabi2 and Belal S Barogaan2
1Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Saudi Arabia
2Faculty of Medicine, Umm Al-Qura University, Saudi Arabia
*Corresponding Author: Abdurazzaq Mohammed Sultan, Biochemistry Department, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia.
Received: January 09, 2020; Published: January 21, 2020




Abstract

The heart utilizes ketone bodies in preference to fatty acids and glucose under normal condition. This study was carried out to investigate the effect of global no-flow ischemia and chemical stress condition on the utilization of D-3-hydroxybutyrate (D-3-HB) and glucose with reference to the AMP-activated protein kinase (AMPK) and p38 mitogen activated protein kinase (p38 MAPK) signaling cascades in the isolated perfused heart. 2,4-dinitrophenol (DNP) and anisomycin (Aniso) are used to mimic hypoxia and ischemia respectively. DNP stimulated D-3-HB and glucose utilization. D-3-HB decreased the DNP-and Insulin-stimulated glucose utilization. The stimulatory effects of Insulin and DNP on glucose utilization were additive. Insulin and DNP enhanced D-3-HB utilization and the stimulatory effects of Insulin and DNP were not additive. Global no-flow ischemia-reperfusion, ionomycin (Iono) an activator of Ca2+-calmodulin-dependent protein kinase kinase (CaMKK) and anisomycin an activator of p38 MAPK stimulate the utilization of D-3-HB. STO-609 and PD-169316 are a selective inhibitor of CaMKK and p38 MAPK respectively. STO-609 and PD-169316 abolished the increase in D-3-HB utilization in response to ionomycin, anisomycin, and global no-flow ischemia-reperfusion. We conclude that these results indicate the involvement of AMPK and p38 MAPK in the regulation of D-3-HB and glucose utilization during stress, and global no-flow ischemia. Stimulatory effect on D-3-HB utilization could be mediated via the AMPK and p38 MAPK signaling cascades.

Keywords: D-3-hydroxybutyrate; Glucose; AMPK; p38 MAPK; Cardiac Metabolism; Ischemia

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Citation: Abdurazzaq Mohammed Sultan., et al. “Global No-Flow Ischemia and Chemical Stress Condition Effects on D-3-hydroxybutyrate and Glucose Utilization in the Isolated Perfused Heart”. EC Emergency Medicine and Critical Care 4.2 (2020): 01-15.

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