Research Article
Volume 4 Issue 12 - 2020
Assessment of Altered Expression of CD81 by Dendritic Cells in HCV-Infected Patients Under Interferon/Ribavirin Therapy
Rania M Saleh*
Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
*Corresponding Author: Rania M Saleh, Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
Received: December 24, 2019; Published: November 28, 2020


Background: Hepatitis C virus (HCV) is a serious global health issue. It is estimated that 130 - 200 million people are chronically infected with HCV worldwide which accounts for 3% of the global population. Combination therapy with Pegylated Interferon and Ribavirin (PEG-IFN/RBV), has shown a low rate of sustained response. HCV genotype 4 (HCV genotype in about 90% of Egyptian patients) shows unfavorable response to combination therapy. Searching for causes of treatment failure has been the focus of many previous studies. CD81 is the most extensively characterized putative HCV receptor and the first HCV host factor to be identified by its ability to interact with a soluble form of HCV-E2 (sE2). The interaction between HCV-E2 and CD81 in vitro causes inhibition of the function of dendritic cells (DC), which affects the immune response to the virus. 

Aim: To study the potential effect of HCV/CD81 interaction on the response to therapy in HCV patients (as a potential mechanism of treatment failure). 

Methods: An analytical cross-sectional study was carried out on 20 healthy controls and 80 Egyptian chronic HCV-patients. Patients were divided into 4 groups according to their response to combination therapy: treatment naïve, EVR, NR and SVR. The results of the following lab tests were obtained from the patients’ records: AST, ALT and HCV-RNA. Assessment of CD81 expression on DC by flow cytometry was done for all groups. 

Results: CD81 expression on DC (CD123+) cells was significantly lower in naive patients compared to control. The non-response to treatment was unrelated to the expression of CD81 on the tested cell; DC (CD11c+ or CD123+). In the meantime, the good response to treatment was not associated with any changes in CD81 expression on DC (CD123+). However, a significant rise in CD81 expression on DC (CD11c+) among responders was found. 

Conclusion: The failure to respond to combination therapy in HCV patients is not related to an altered expression of CD81 on both DC subsets. On the contrary, the alterations in CD81 expression on CD11c+ DC in those patients could be relevant for favorable control of HCV infection and favorable treatment response.

Keywords: CD81; Dendritic Cells; PBMC


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Citation: Rania M Saleh. “Assessment of Altered Expression of CD81 by Dendritic Cells in HCV-Infected Patients Under Interferon/Ribavirin Therapy”. EC Emergency Medicine and Critical Care 4.12 (2020): 01-13.

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